Abstract
Enzyme-induced calcite precipitation (EICP) is a biocementation technique that has the potential to improve the engineering properties of sand. The effectiveness of the EICP treatment was evaluated based on the unconfined compressive strength (UCS) tests at various concentrations of cementation reagent (CCR) and curing periods. The treated sand was analysed for its calcium carbonate content and microstructural analysis using FESEM-EDX. The results showed an increase in unconfined compressive strength and calcium carbonate content at a higher concentration of cementation reagent. The UCS value and CaCO3 content of the treated samples are 161–552 kPa and 0.92–5.73%, respectively. There is a linear relationship between the UCS at various cementation reagent concentrations and the average calcium carbonate content.
Similar content being viewed by others
References
Akbarimehr D, Aflaki E, Eslami A (2019) Experimental investigation of the densification properties of clay soil mixes with tire waste. Civ Eng J 5:363–372. https://doi.org/10.28991/cej-2019-03091251
Çelik S, Majedi P, Akbulut S (2019) Granular soil improvement by using polyester grouts. Iran J Sci Technol Trans Civ Eng 43:599–606. https://doi.org/10.1007/s40996-018-0203-3
Karol RH, Reuben H et al (2003) Chemical grouting and soil stabilization. CRC Press, New York
Mahawish A, Bouazza A, Gates WP (2018) Improvement of coarse sand engineering properties by microbially induced calcite precipitation. Geomicrobiol J 35:887–897. https://doi.org/10.1080/01490451.2018.1488019
Dejong JT, Kavazanjian E (2019) Bio-mediated and bio-inspired geotechnics. Lu N, Mitchell J (Eds.) In: Geotechnical fundamentals for addressing new world challenges. Springer, pp 193–207
Wen K, Li Y, Amini F, Li L (2020) Impact of bacteria and urease concentration on precipitation kinetics and crystal morphology of calcium carbonate. Acta Geotech 15:17–27. https://doi.org/10.1007/s11440-019-00899-3
Kong HTP, Kassim KA, Umar M (2019) Microbially induced carbonate precipitations to improve residual soil at various temperatures. Bull Geol Soc Malaysia 67:75–81. https://doi.org/10.7186/bgsm67201909
Osinubi KJ, Eberemu AO, Ijimdiya TS (2020) Review of the use of microorganisms in geotechnical engineering applications. SN Appl Sci 2:1–19. https://doi.org/10.1007/s42452-020-1974-2
Dilrukshi RAN, Kawasaki S (2019) Effect of plant-derived urease-induced carbonate formation on the strength enhancement of sandy soil. In: Achal V, Abhijit M (eds) Ecological wisdom inspired restoration engineering. Springer, Singapore, p 93–108
Javadi N, Khodadadi H, Hamdan N, Kavazanjian E Jr (2018) EICP treatment of soil by using urease enzyme extracted from watermelon seeds. In: IFCEE 2018 GSP 296, pp 115–124
Putra H, Yasuhara H, Kinoshita N (2017) Optimum Condition for the application of enzyme-mediated calcite precipitation technique as soil improvement method. Int J Adv Sci Eng Inf Technol 7:2145–2151
Almajed A, Tirkolaei HK, Edward KJ, Hamdan N (2019) Enzyme induced biocementated sand with high strength at low carbonate content. Sci Rep. https://doi.org/10.1038/s41598-018-38361-1
Nemati M, Voordouw G (2003) Modification of porous media permeability, using calcium carbonate produced enzymatically in situ. Enzyme Microb Technol 33:635–642. https://doi.org/10.1016/S0141-0229(03)00191-1
Muhammed SA, Kassim KA, Ahmad K, Zango MU, Chong CS, Makinda J (2021) Influence of multiple treatment cycles on the strength and microstructure of biocemented sandy soil. Int J Environ Sci Technol 1–10. https://doi.org/10.1007/s13762-020-03073-5
Park SS, Choi SG, Nam IH (2014) Effect of plant-induced calcite precipitation on the strength of sand. J Mater Civ Eng 26:1–5. https://doi.org/10.1061/(ASCE)MT.1943-5533.0001029
Krajewska B (2018) Urease-aided calcium carbonate mineralization for engineering applications: a review. J Adv Res 13:59–67. https://doi.org/10.1016/j.jare.2017.10.009
Sun X, Miao L, Wu L, Sun X (2020) Applicability and theoretical calculation of enzymatic calcium carbonate precipitation for sand improvement. Geomicrobiol J 37:389–399. https://doi.org/10.1080/01490451.2019.1710625
Carmona JPSF, Oliveira PJV (2017) Improvement of a sandy soil by enzymatic calcium carbonate precipitation. Geotech Eng 171:3–15
Neupane D, Yasuhara H, Kinoshita N, Unno T (2013) Applicability of enzymatic calcium carbonate precipitation as a soil-strengthening technique. J Geotech Geoenviron Eng 139:2201–2212. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000959
Yasuhara H, Neupane D, Hayashi K, Okamura M (2012) Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation. Soils Found 52:539–549. https://doi.org/10.1016/j.sandf.2012.05.011
Kavazanjian E, Hamdan N (2015) Enzyme induced carbonate precipitation (EICP) columns for ground improvement. In: IFCEE 2015—geotechnical special publication, pp 2252–2261
Almajed A, Hamed KT, Edward KJ (2018) Baseline investigation on enzyme-induced calcium carbonate precipitation. J Geotech Geoenviron Eng 144:1–11. https://doi.org/10.1061/(ASCE)GT.1943-5606.0001973
Arab M, Omar M, Aljassmi R (2019) EICP cemented sand modified with biopolymer. In: International congress and exhibition" sustainable civil infrastructures, pp 74–85
British Standards Institution (2010) BS 1377-2:1990 methods of test for soils for civil engineering purposes. Part 2: classification tests. Br St and 68
Ghasemi P, Zamani A, Montoya B (2019) The effect of chemical concentration on the strength and erodibility of MICP treated sands. Geo-Congress 2019:143–151
Omoregie AI, Palombo EA, Ong DELL, Nissom PM (2019) Biocementation of sand by Sporosarcina pasteurii strain and technical-grade cementation reagents through surface percolation treatment method. Constr Build Mater 228:116828. https://doi.org/10.1016/j.conbuildmat.2019.116828
Montoya BM, DeJong JT (2015) Stress-strain behavior of sands cemented by microbially induced calcite precipitation. J Geotech Geoenviron Eng 141:1–10. https://doi.org/10.1061/(ASCE)GT.1943-5606
Rohy H, Arab M, Zeiada W (2019) One phase soil bio-cementation with EICP-soil mixing. In: Proceedings of the 4th world congress on civil, structural, and environmental engineering (CSEE’19), pp 1–8
Kawasaki S, Akiyama M (2013) Enhancement of unconfined compressive strength of sand test pieces cemented with calcium phosphate compound by addition of various powders. Soils Found 53:966–976. https://doi.org/10.1016/j.sandf.2013.10.013
Van Der RM, Van der Zon W (2009) Biological in situ reinforcement of sand in near-shore areas. Proc Inst Civ Eng Eng 162:81–83. https://doi.org/10.1680/geng.2009.162.1.81
Gowthaman S, Mitsuyama S, Nakashima K (2019) Microbial induced slope surface stabilization using industrial-grade chemicals: a preliminary. Int J Geomate 17:110–116. https://doi.org/10.21660/2019.60.8150
Dhami NK, Reddy MS, Mukherjee A (2013) Biomineralization of calcium carbonates and their engineered applications: a review. Front Microbiol 4:1–13
Zhao Q, Li L, Li C (2014) Factors affecting improvement of engineering properties of MICP-treated soil catalyzed by bacteria and urease. J Mater Civ Eng 26:1–10. https://doi.org/10.1061/(ASCE)MT.1943-5533
Nafisi A, Safavizadeh S, Montoya BM (2019) Influence of microbe and enzyme-induced treatments on cemented sand shear response. J Geotech Geoenviron Eng 145:1–8. https://doi.org/10.1061/(ASCE)GT.1943-5606.0002111
Li D, Tian K, Zhang H (2018) Experimental investigation of solidifying desert aeolian sand using microbially induced calcite precipitation. Constr Build Mater 172:251–262. https://doi.org/10.1016/j.conbuildmat.2018.03.255
Dadda A, Geindreau C, Emeriault F, Rolland S (2018) Characterization of contact properties in biocemented sand using 3D. Acta Geotech. https://doi.org/10.1007/s11440-018-0744-4
DeJong JT, Mortensen BM, Martinez BC, Nelson DC (2010) Bio-mediated soil improvement. Ecol Eng 36:197–210. https://doi.org/10.1016/j.ecoleng.2008.12.029
MEEI (2014) Energy dispersive X-ray spectroscopy—handbook of analytical methods for materials. Mater EvalEng Inc, Plymouth, MN, USA, 17–18
Harkes MP, Van PLA, Booster JL (2010) Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement. Ecol Eng J 36:112–117. https://doi.org/10.1016/j.ecoleng.2009.01.004
Nam I, Chon C, Jung K, Choi S (2015) Calcite precipitation by ureolytic plant (Canavalia ensiformis) extracts as effective biomaterials. KSCE J Civ Eng 19:1620–1625. https://doi.org/10.1007/s12205-014-0558-3
Funding
The authors would like to acknowledge with appreciation the support by Malaysian Ministry of Higher Education for providing the Fundamental Research Grant Scheme (Grant no. 5F256). Similarity grant from Universiti Teknologi Malaysia (Grant no. 20H21) is greatly acknowledge. The first and third authors also appreciate scholarship granted by TETFUND Nigeria.
Author information
Authors and Affiliations
Contributions
ASM conducted most of the experiment and wrote the manuscript. KAK reviewed and confirmed the final version of the manuscript. MUZ conducted part of the experiment and analysed the results. KA reviewed and edited the manuscript. JM conducted part of the analyses of the results.
Corresponding author
Ethics declarations
Conflict of interest
We declare there is no conflict of interest in connection with this study.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Muhammed, A.S., Kassim, K.A., Zango, M.U. et al. Enhancing the Strength of Sandy Soil Through Enzyme-Induced Calcite Precipitation. Int. J. of Geosynth. and Ground Eng. 7, 45 (2021). https://doi.org/10.1007/s40891-021-00289-4
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s40891-021-00289-4