Skip to main content
Log in

Enhancing the Strength of Sandy Soil Through Enzyme-Induced Calcite Precipitation

  • Technical Note
  • Published:
International Journal of Geosynthetics and Ground Engineering Aims and scope Submit manuscript

Abstract

Enzyme-induced calcite precipitation (EICP) is a biocementation technique that has the potential to improve the engineering properties of sand. The effectiveness of the EICP treatment was evaluated based on the unconfined compressive strength (UCS) tests at various concentrations of cementation reagent (CCR) and curing periods. The treated sand was analysed for its calcium carbonate content and microstructural analysis using FESEM-EDX. The results showed an increase in unconfined compressive strength and calcium carbonate content at a higher concentration of cementation reagent. The UCS value and CaCO3 content of the treated samples are 161–552 kPa and 0.92–5.73%, respectively. There is a linear relationship between the UCS at various cementation reagent concentrations and the average calcium carbonate content.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Akbarimehr D, Aflaki E, Eslami A (2019) Experimental investigation of the densification properties of clay soil mixes with tire waste. Civ Eng J 5:363–372. https://doi.org/10.28991/cej-2019-03091251

    Article  Google Scholar 

  2. Çelik S, Majedi P, Akbulut S (2019) Granular soil improvement by using polyester grouts. Iran J Sci Technol Trans Civ Eng 43:599–606. https://doi.org/10.1007/s40996-018-0203-3

    Article  Google Scholar 

  3. Karol RH, Reuben H et al (2003) Chemical grouting and soil stabilization. CRC Press, New York

  4. Mahawish A, Bouazza A, Gates WP (2018) Improvement of coarse sand engineering properties by microbially induced calcite precipitation. Geomicrobiol J 35:887–897. https://doi.org/10.1080/01490451.2018.1488019

    Article  Google Scholar 

  5. Dejong JT, Kavazanjian E (2019) Bio-mediated and bio-inspired geotechnics. Lu N, Mitchell J (Eds.) In: Geotechnical fundamentals for addressing new world challenges. Springer, pp 193–207

  6. Wen K, Li Y, Amini F, Li L (2020) Impact of bacteria and urease concentration on precipitation kinetics and crystal morphology of calcium carbonate. Acta Geotech 15:17–27. https://doi.org/10.1007/s11440-019-00899-3

    Article  Google Scholar 

  7. Kong HTP, Kassim KA, Umar M (2019) Microbially induced carbonate precipitations to improve residual soil at various temperatures. Bull Geol Soc Malaysia 67:75–81. https://doi.org/10.7186/bgsm67201909

    Article  Google Scholar 

  8. Osinubi KJ, Eberemu AO, Ijimdiya TS (2020) Review of the use of microorganisms in geotechnical engineering applications. SN Appl Sci 2:1–19. https://doi.org/10.1007/s42452-020-1974-2

    Article  Google Scholar 

  9. Dilrukshi RAN, Kawasaki S (2019) Effect of plant-derived urease-induced carbonate formation on the strength enhancement of sandy soil. In: Achal V, Abhijit M (eds) Ecological wisdom inspired restoration engineering. Springer, Singapore, p 93–108

    Chapter  Google Scholar 

  10. Javadi N, Khodadadi H, Hamdan N, Kavazanjian E Jr (2018) EICP treatment of soil by using urease enzyme extracted from watermelon seeds. In: IFCEE 2018 GSP 296, pp 115–124

  11. Putra H, Yasuhara H, Kinoshita N (2017) Optimum Condition for the application of enzyme-mediated calcite precipitation technique as soil improvement method. Int J Adv Sci Eng Inf Technol 7:2145–2151

    Article  Google Scholar 

  12. Almajed A, Tirkolaei HK, Edward KJ, Hamdan N (2019) Enzyme induced biocementated sand with high strength at low carbonate content. Sci Rep. https://doi.org/10.1038/s41598-018-38361-1

    Article  Google Scholar 

  13. Nemati M, Voordouw G (2003) Modification of porous media permeability, using calcium carbonate produced enzymatically in situ. Enzyme Microb Technol 33:635–642. https://doi.org/10.1016/S0141-0229(03)00191-1

    Article  Google Scholar 

  14. Muhammed SA, Kassim KA, Ahmad K, Zango MU, Chong CS, Makinda J (2021) Influence of multiple treatment cycles on the strength and microstructure of biocemented sandy soil. Int J Environ Sci Technol 1–10. https://doi.org/10.1007/s13762-020-03073-5

  15. Park SS, Choi SG, Nam IH (2014) Effect of plant-induced calcite precipitation on the strength of sand. J Mater Civ Eng 26:1–5. https://doi.org/10.1061/(ASCE)MT.1943-5533.0001029

    Article  Google Scholar 

  16. Krajewska B (2018) Urease-aided calcium carbonate mineralization for engineering applications: a review. J Adv Res 13:59–67. https://doi.org/10.1016/j.jare.2017.10.009

    Article  Google Scholar 

  17. Sun X, Miao L, Wu L, Sun X (2020) Applicability and theoretical calculation of enzymatic calcium carbonate precipitation for sand improvement. Geomicrobiol J 37:389–399. https://doi.org/10.1080/01490451.2019.1710625

    Article  Google Scholar 

  18. Carmona JPSF, Oliveira PJV (2017) Improvement of a sandy soil by enzymatic calcium carbonate precipitation. Geotech Eng 171:3–15

    Article  Google Scholar 

  19. Neupane D, Yasuhara H, Kinoshita N, Unno T (2013) Applicability of enzymatic calcium carbonate precipitation as a soil-strengthening technique. J Geotech Geoenviron Eng 139:2201–2212. https://doi.org/10.1061/(ASCE)GT.1943-5606.0000959

    Article  Google Scholar 

  20. Yasuhara H, Neupane D, Hayashi K, Okamura M (2012) Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation. Soils Found 52:539–549. https://doi.org/10.1016/j.sandf.2012.05.011

    Article  Google Scholar 

  21. Kavazanjian E, Hamdan N (2015) Enzyme induced carbonate precipitation (EICP) columns for ground improvement. In: IFCEE 2015—geotechnical special publication, pp 2252–2261

  22. Almajed A, Hamed KT, Edward KJ (2018) Baseline investigation on enzyme-induced calcium carbonate precipitation. J Geotech Geoenviron Eng 144:1–11. https://doi.org/10.1061/(ASCE)GT.1943-5606.0001973

    Article  Google Scholar 

  23. Arab M, Omar M, Aljassmi R (2019) EICP cemented sand modified with biopolymer. In: International congress and exhibition" sustainable civil infrastructures, pp 74–85

  24. British Standards Institution (2010) BS 1377-2:1990 methods of test for soils for civil engineering purposes. Part 2: classification tests. Br St and 68

  25. Ghasemi P, Zamani A, Montoya B (2019) The effect of chemical concentration on the strength and erodibility of MICP treated sands. Geo-Congress 2019:143–151

    Google Scholar 

  26. Omoregie AI, Palombo EA, Ong DELL, Nissom PM (2019) Biocementation of sand by Sporosarcina pasteurii strain and technical-grade cementation reagents through surface percolation treatment method. Constr Build Mater 228:116828. https://doi.org/10.1016/j.conbuildmat.2019.116828

    Article  Google Scholar 

  27. Montoya BM, DeJong JT (2015) Stress-strain behavior of sands cemented by microbially induced calcite precipitation. J Geotech Geoenviron Eng 141:1–10. https://doi.org/10.1061/(ASCE)GT.1943-5606

    Article  Google Scholar 

  28. Rohy H, Arab M, Zeiada W (2019) One phase soil bio-cementation with EICP-soil mixing. In: Proceedings of the 4th world congress on civil, structural, and environmental engineering (CSEE’19), pp 1–8

  29. Kawasaki S, Akiyama M (2013) Enhancement of unconfined compressive strength of sand test pieces cemented with calcium phosphate compound by addition of various powders. Soils Found 53:966–976. https://doi.org/10.1016/j.sandf.2013.10.013

    Article  Google Scholar 

  30. Van Der RM, Van der Zon W (2009) Biological in situ reinforcement of sand in near-shore areas. Proc Inst Civ Eng Eng 162:81–83. https://doi.org/10.1680/geng.2009.162.1.81

    Article  Google Scholar 

  31. Gowthaman S, Mitsuyama S, Nakashima K (2019) Microbial induced slope surface stabilization using industrial-grade chemicals: a preliminary. Int J Geomate 17:110–116. https://doi.org/10.21660/2019.60.8150

    Article  Google Scholar 

  32. Dhami NK, Reddy MS, Mukherjee A (2013) Biomineralization of calcium carbonates and their engineered applications: a review. Front Microbiol 4:1–13

    Article  Google Scholar 

  33. Zhao Q, Li L, Li C (2014) Factors affecting improvement of engineering properties of MICP-treated soil catalyzed by bacteria and urease. J Mater Civ Eng 26:1–10. https://doi.org/10.1061/(ASCE)MT.1943-5533

    Article  Google Scholar 

  34. Nafisi A, Safavizadeh S, Montoya BM (2019) Influence of microbe and enzyme-induced treatments on cemented sand shear response. J Geotech Geoenviron Eng 145:1–8. https://doi.org/10.1061/(ASCE)GT.1943-5606.0002111

    Article  Google Scholar 

  35. Li D, Tian K, Zhang H (2018) Experimental investigation of solidifying desert aeolian sand using microbially induced calcite precipitation. Constr Build Mater 172:251–262. https://doi.org/10.1016/j.conbuildmat.2018.03.255

    Article  Google Scholar 

  36. Dadda A, Geindreau C, Emeriault F, Rolland S (2018) Characterization of contact properties in biocemented sand using 3D. Acta Geotech. https://doi.org/10.1007/s11440-018-0744-4

    Article  Google Scholar 

  37. DeJong JT, Mortensen BM, Martinez BC, Nelson DC (2010) Bio-mediated soil improvement. Ecol Eng 36:197–210. https://doi.org/10.1016/j.ecoleng.2008.12.029

    Article  Google Scholar 

  38. MEEI (2014) Energy dispersive X-ray spectroscopy—handbook of analytical methods for materials. Mater EvalEng Inc, Plymouth, MN, USA, 17–18

  39. Harkes MP, Van PLA, Booster JL (2010) Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement. Ecol Eng J 36:112–117. https://doi.org/10.1016/j.ecoleng.2009.01.004

    Article  Google Scholar 

  40. Nam I, Chon C, Jung K, Choi S (2015) Calcite precipitation by ureolytic plant (Canavalia ensiformis) extracts as effective biomaterials. KSCE J Civ Eng 19:1620–1625. https://doi.org/10.1007/s12205-014-0558-3

    Article  Google Scholar 

Download references

Funding

The authors would like to acknowledge with appreciation the support by Malaysian Ministry of Higher Education for providing the Fundamental Research Grant Scheme (Grant no. 5F256). Similarity grant from Universiti Teknologi Malaysia (Grant no. 20H21) is greatly acknowledge. The first and third authors also appreciate scholarship granted by TETFUND Nigeria.

Author information

Authors and Affiliations

Authors

Contributions

ASM conducted most of the experiment and wrote the manuscript. KAK reviewed and confirmed the final version of the manuscript. MUZ conducted part of the experiment and analysed the results. KA reviewed and edited the manuscript. JM conducted part of the analyses of the results.

Corresponding author

Correspondence to Abubakar Sadiq Muhammed.

Ethics declarations

Conflict of interest

We declare there is no conflict of interest in connection with this study.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Muhammed, A.S., Kassim, K.A., Zango, M.U. et al. Enhancing the Strength of Sandy Soil Through Enzyme-Induced Calcite Precipitation. Int. J. of Geosynth. and Ground Eng. 7, 45 (2021). https://doi.org/10.1007/s40891-021-00289-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s40891-021-00289-4

Keywords

Navigation