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Antibacterial Activity of Silver Nanoparticles Derived from Extracellular Extract of Enterococcus aerogenes Against Dental Disease Bacteria Isolated

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Abstract

Purpose

Bacteria are known to have a high ability to manufacture many compounds with biological functions in a short time compared with eukaryotic cells due to the fact that bacterial cells possess efficient metabolic mechanisms for the manufacture of these compounds (intracellular or extracellular). Herein, the goal of this study is to use pathogenic Enterococcus aerogenes bacteria strains, namely, S1, S2, and S3, isolated from the mouths of individuals with dental decay to produce silver nanoparticles in an environmentally friendly and cost-effective manner.

Methods

These nanoparticles have been tested for antibacterial activity against Streptococcus mitis, an MDR bacterium, either alone or in combination with antibiotics. These bacteria were identified using morphological characteristics and biochemical tests, in addition to molecular methods such as PCR and DNA sequences. Besides, their identification was done on the basis of their alignment with the reference strains in the NCBI blast to calculate the degree of similarity among these strains (S1, S2, and S3).

Results

The results of the current study showed a clear synergistic effect in the inhibition of Streptococcus mitis bacteria when mixing silver nanoparticles with some antibiotics, and it was found that there is a synergistic effect when mixing those AgNPs with erythromycin, followed by streptomycin and tetracycline. In contrast, the effect was antagonistic in the case of streptomycin and tetracycline antibiotics.

Conclusion

Enterobacter aerogenes AgNPs demonstrated excellent antibacterial efficacy on Streptococcus mitis isolates. Therefore, AgNPs in the dental care area have a wide range of applications.

Lay Summary

The current study attempted to show how AgNPs have a broad range of uses in the dental care field. Therefore, the study employed AgNPs that were created by Enterobacter aerogenes bacterial strains (S1, S2, and S3) for dental caries patients. AgNPs from Enterobacter aerogenes exhibited strong antibacterial activity against Streptococcus mitis isolates.

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Authors and Affiliations

  1. Department of Biology, College of Science, University of Misan, Misan, Amarah, Iraq

    Mohammed A. Abd Ali

  2. Department of Biology, College of education for pure sciences, University of Basra, Basra, Iraq

    Ali Aboud Shareef

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  1. Mohammed A. Abd Ali
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  2. Ali Aboud Shareef
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Correspondence to Mohammed A. Abd Ali.

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Abd Ali, M.A., Shareef, A.A. Antibacterial Activity of Silver Nanoparticles Derived from Extracellular Extract of Enterococcus aerogenes Against Dental Disease Bacteria Isolated. Regen. Eng. Transl. Med. 10, 68–77 (2024). https://doi.org/10.1007/s40883-023-00304-2

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  • DOI: https://doi.org/10.1007/s40883-023-00304-2

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