Abstract
Daphne mucronata (DM) has regenerative features. Alginate hydrogel (Alg), as a natural scaffold, acts as a drug delivery system. This study aimed to assess the probable regenerative effects of DM encapsulated in Alg dermal scaffold on skin wound healing. Seventy-two male mice were grouped as: control (normal skin with no experimental treatments), sham (only punched skin), margarine (only margarine ointment administration), Alg group (Alg hydrogel with no DM), margarine-DM (margarine-based ointment with DM), and Alg-DM (DM encapsulated in Alg scaffold). DPPH assay was applied for phytochemical screening of DM. Cranial and cranial incisions of back were conducted for histological and gene expression evaluations, respectively. Alg scaffold was placed on wound and supported by a dressing pad. Tissue sampling was applied on 3, 7, 12, and 22 days of treatments. Collagen deposition, regeneration rate, angiogenesis and granulation, respectively, were assessed through hydroxyproline assay, caliper instrument, CD31 immunohistochemistry staining, and ImageJ software. Also, gene expression of MMP13, EGF, and bFGF was evaluated by real-time PCR. Data were analyzed using SPSS (V. 16), and graphs were drawn by GraphPad Prism (V.9, 2020) software. Antioxidant agents of DM were approved by the DPPH assay. Besides, it was found that DM restored the damaged dermal tissue and increased the expression of genes involved in wound healing significantly (p < 0.05) in 7 and 12 days of experiment in both groups of margarine-DM and Alg-DM than control. Application of hydroalcoholic extract of DM encapsulated in alginate hydrogel scaffold can accelerate skin tissue regeneration by induction of genes expression and histological tissue indices in dermal injuries.
It is believed that the drug delivery system can increase the effectiveness of a drug. Application of dermal scaffold leads to the early expression of genes involved in wound healing and changes of histopathological factors. This study found that the Daphne mucronata extract encapsulated in alginate gel can accelerate wound healing by modulation of the expression of genes and tissue factors. This technique can be used in other pathologic skin-related conditions like diabetes or other non-skin injuries like musculoskeletal disorders.
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This work was supported by grant No.97151 by Deputy of Research of KUMS, Kermanshah, Iran.
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This study was designed by MR. Gh. as a corresponding author. S. A. K. as leader of research group managed the experimental protocols. A. S., S. N., and M. A. were involved in practical laboratory procedures. The manuscript was prepared by A. A. and confirmed by all authors.
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Karimi, S.A., Abdolmaleki, A., Sadeghi, A. et al. Drug Delivery System Through Alginate Dermal Scaffold Loaded with Hydroalcoholic Extract of Daphne Mucronata Improves Dermal Excisional Wound Healing: An Experimental Research. Regen. Eng. Transl. Med. 8, 523–534 (2022). https://doi.org/10.1007/s40883-022-00252-3
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DOI: https://doi.org/10.1007/s40883-022-00252-3