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Current species of oomycetes associated with foot rot disease of black pepper in Vietnam

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Abstract

Foot rot disease caused by Phytophthora capsici is one of the most destructive diseases of black pepper in Vietnam and worldwide. However, other oomycete species such as P. tropicalis and Pythium deliense reported as serious threats to black pepper in India have also been recorded on this plant. The population of oomycetes occurring in black pepper plantations in Vietnam and their pathogenicity have not been investigated in the last decade. To this end, two hundred fifteen oomycete isolates were collected from the root rots and rhizospheric soil of black pepper in the Central Highlands and the Southeast region of Vietnam. Of these, 23 isolates were representatively chosen based on their origin and morphology for DNA sequence analysis of the internal transcribed spacer region, then 11 isolates were further selected for the translation elongation factor 1-alpha and the beta-tubulin gene analyses. Morphology and molecular analyses indicated that P. capsici, P. cinnamomi, P. heveae, P. nicotianae, P. parvispora, P. tropicalis, Phytopythium vexans, and a new species candidate Phytopythium sp. were identified among oomycete isolates. Of these, P. capsici and P. tropicalis could be the prevalent species in black pepper plantations in studied areas. The inoculation tests demonstrated that P. capsici, P. nicotianae and P. tropicalis were pathogenic on both leaves and roots of black pepper. Phytopythium vexans was pathogenic on root only. Meanwhile, P. cinnamomi, P. heveae, P. parvispora and Phytopythium sp. were non-pathogenic.

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Data availability

The sequence data obtained in this study were deposited in GenBank with accession numbers listed in Table 1.

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Acknowledgements

The authors would like to thank the Australian Centre for International Agricultural Research, Australia for financial support (Project no. AGB/2018/175).

Funding

Australian Centre for International Agricultural Research, AGB/2018/175, Le Dinh Thao.

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Authors and Affiliations

  1. Plant Protection Research Institute (PPRI), Academy of Agricultural Sciences (VAAS), Duc Thang, Bac Tu Liem, Hanoi, Vietnam

    Le Dinh Thao, Tran Ngoc Khanh, Nguyen Van Liem, Le Thu Hien, Ha Minh Thanh, Vu Thi Phuong Binh, Thieu Thi Thu Trang, Pham Thi Anh, Nguyen Van Chung & Pham Hong Hien

  2. Research and Development Pepper Centre, The Western Highland Agriculture and Forestry Science Institute, Pleiku City, Vietnam

    Nguyen Van Long

  3. School of Life and Environmental Sciences, Faculty of Science, Engineering and Built Environment, Deakin University, Geelong, Victoria, Australia

    Nguyen Van Long, Nguyen Quang Duy, Didier Lesueur, Laetitia Herrmann & Lambert Brau

  4. Alliance of Bioversity International and International Center for Tropical Agriculture (CIAT), Common Microbial Biotechnology Platform (CMBP), Asia hub, Hanoi, Vietnam

    Nguyen Van Long, Nguyen Quang Duy, Didier Lesueur & Laetitia Herrmann

  5. Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR Eco&Sols, Hanoi, Vietnam

    Didier Lesueur

  6. l’ Alimentation et l’Environnement (INRAE), Institut de Recherche pour le Développement (IRD), Eco&Sols, Université de Montpellier (UMR), CIRAD, Institut National de Recherche pour l’Agriculture, 34060, Montpellier, France, Montpellier SupAgro

    Didier Lesueur

  7. Chinese Academy of Tropical Agricultural Sciences, Rubber Research Institute, Haikou, China

    Didier Lesueur

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  1. Le Dinh Thao
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  2. Tran Ngoc Khanh
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Contributions

Sample collection and isolation: Le Dinh Thao, Tran Ngoc Khanh, Nguyen Van Liem, Le Thu Hien, Ha Minh Thanh, Vu Thi Phuong Binh, Thieu Thi Thu Trang, Pham Thi Anh, Nguyen Van Chung, Pham Hong Hien, Nguyen Van Long, Nguyen Quang Duy. Data analysis: Le Dinh Thao, Thieu Thi Thu Trang. Writing of draft manuscript: Le Dinh Thao. Review and editing: Didier Lesueur, Laetitia Herrmann, Lambert Brau. All authors read and approved the final manuscript.

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Thao, L.D., Khanh, T.N., Van Liem, N. et al. Current species of oomycetes associated with foot rot disease of black pepper in Vietnam. Trop. plant pathol. 49, 633–648 (2024). https://doi.org/10.1007/s40858-024-00662-4

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