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Bacterial leaf streak: Inoculation methodology determination

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Abstract

Information on inoculation methodologies and the phenological stage at which corn is susceptible to infection by Xanthomonas vasicola pv. vasculorum, the etiological agent of bacterial streak, are scarce. The objectives of the work were to model the calibration curve of the bacteria inoculum that allows the evaluation of the physiological damage in corn and determine its inoculation methodology. The calibration curve was obtained by dish counting the inoculum concentration. For the inoculation methodology, three sequential experiments were performed: inoculation method (absolute control, wind friction, steel wool, multi-needles and no injury, on the adaxial and abaxial sides of corn leaves), inoculum concentration (non-inoculated control, absorbances from 0.2 to 0.8 in a spectrophotometer at 580 nm) and phenological stage (non-inoculated control, inoculations via seed, in V4, in V8, in VT and in R2). For the disease to express itself, around 1 × 108 CFU/mL were necessary. Multi-needle treatments and inoculation without causing injury differed significantly from the others, showing greater severity. Inoculation on the adaxial surface was more severe than on the abaxial surface. The symptoms of the disease started at a concentration of 0.4 and the severity of the bacterial streak was more intense at a concentration of 0.6, not differing from the higher concentration. Regarding the most susceptible phenological phase to the bacteria, the observed incubation period was 72 h and there were significant differences only in relation to the incidence expression. Stage V8 was considered the most susceptible to infection.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank CAPES (Coordination for the Improvement of Higher Education Personnel) for providing a scholarship to the first author and CNPq (National Council for Scientific and Technological Development) for granting scholarships to the fifth and sixth authors.

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Authors and Affiliations

  1. Programa de Pós-Graduação Em Agronomia, Universidade Estadual Do Centro-Oeste, CEP 85040-167, Guarapuava, Paraná, Brasil

    Francine Lautenchleger, Marcos Ventura Faria, Cacilda Marcia Duarte Rios Faria, Emanuel Gava & Elis Marina Müller Silva

  2. Departamento de Biologia, Universidade Estadual de Feira de Santana, CEP 44036-900, Feira de Santana, Bahia, Brasil

    Leandro Alvarenga Santos

  3. Departamento de Agronomia, Universidade Estadual Do Centro-Oeste, CEP 85040-167, Guarapuava, Paraná, Brasil

    Laís Maximo Gasperotto & Pedro Henrique Willemann Andreoli

Authors
  1. Francine Lautenchleger
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  2. Marcos Ventura Faria
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  3. Cacilda Marcia Duarte Rios Faria
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  4. Leandro Alvarenga Santos
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  5. Laís Maximo Gasperotto
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  6. Pedro Henrique Willemann Andreoli
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  7. Emanuel Gava
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  8. Elis Marina Müller Silva
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Cacilda Marcia Duarte Rios Faria, Elis Müller, Emanuel Gava, Francine Lautenchleger, Laís Maximo Gasperotto, Leandro Alvarenga Santos, Marcos Ventura Faria and Pedro Henrique Willemann Andreoli. The first draft of the manuscript was written by Francine Lautenchleger and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Francine Lautenchleger.

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Lautenchleger, F., Faria, M.V., Faria, C.M.D.R. et al. Bacterial leaf streak: Inoculation methodology determination. Trop. plant pathol. 48, 720–727 (2023). https://doi.org/10.1007/s40858-023-00611-7

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  • DOI: https://doi.org/10.1007/s40858-023-00611-7

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