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Pepper mild mottle virus isolates from Peru induce severe symptoms in susceptible pepper plants and belong to the P1,2 pathotype

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Abstract

Pepper mild mottle virus (PMMoV), a tobamovirus, has been reported worldwide infecting, especially, pepper plants. In this study, seven new PMMoV isolates were identified in symptomatic pepper plants collected in four departments of Peru. The majority of these isolates induced severe symptoms, including mottle, in susceptible pepper plants. Inoculation on pepper plants carrying four known functional L resistance alleles (L1, L2, L3, and L4) revealed that PMMoV isolates from Peru belong to the P1,2 pathotype. Therefore, pepper species or cultivars with L3 and L4 alleles displayed hypersensitive response after mechanical inoculation and were still resistant to PMMoV isolates circulating in Peru. Infection by PMMoV was monitored by serology (DAS-ELISA) and RT-PCR with degenerate primers. Virus genes were used to build a phylogenetic tree that showed diversity accumulation among the PMMoV isolates from Peru.

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Data availability

The datasets generated during and/or analyzed during the current study are available in the NCBI repository [https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode =Info&id=12239].

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Acknowledgments

We would like to thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF), and Secretaria de Educación Superior, Ciencia, Tecnología e Innovación-SENESCYT for financial support.

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Authors and Affiliations

  1. Departamento de Fitopatologia, Universidade de Brasília, Brasília, DF, 70910-900, Brazil

    Jefferson B. Vélez-Olmedo & Fernando L. Melo

  2. Facultad de Ingeniería Agronómica, Departamento de Ciencias Agronómicas, Universidad Técnica de Manabí, 130105, Portoviejo, Ecuador

    Jefferson B. Vélez-Olmedo

  3. Departamento Académico de Fitopatología, Universidad Nacional Agraria la Molina, Apartado 456, Lima, Peru

    Cesar E. Fribourg

  4. Dapartamento de Biologia Celular, Universidade de Brasília, Brasília, DF, 70910-900, Brazil

    Tatsuya Nagata, Athos S. de Oliveira & Renato O. Resende

Authors
  1. Jefferson B. Vélez-Olmedo
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  2. Cesar E. Fribourg
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  3. Fernando L. Melo
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  4. Tatsuya Nagata
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  5. Athos S. de Oliveira
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  6. Renato O. Resende
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Contributions

All authors intellectually contributed to this study. Design of experiments was performed by J.B.V.O., C.E.F., and R.O.R. Collection in the field was done by J.B.V.O. and C.E.F. Experiments were performed by J.B.V.O., C.E.F., and T.N. Data analysis was performed by J.B.V.O., A.S.O., and F.L.M. Writing of original draft was performed by J.B.V.O., A.S.O., and R.O.R. Resources were supplied by R.O.R. All authors read and commented the manuscript versions. All authors read and approved the final manuscript.

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Correspondence to Renato O. Resende.

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Vélez-Olmedo, J.B., Fribourg, C.E., Melo, F.L. et al. Pepper mild mottle virus isolates from Peru induce severe symptoms in susceptible pepper plants and belong to the P1,2 pathotype. Trop. plant pathol. 46, 381–385 (2021). https://doi.org/10.1007/s40858-020-00402-4

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  • DOI: https://doi.org/10.1007/s40858-020-00402-4

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