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Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection

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Abstract

Tomato chlorosis virus (ToCV) is a whitefly-transmitted crinivirus that causes yield losses, mainly in tomato (Solanum lycopersicum) and potato (S. tuberosum) crops. In this work, a polyclonal antiserum for detecting the virus using a dot-blot immunoassay was developed and the responses of crinivirus-infected potato genotypes were evaluated. The virus was purified using infected tomato leaves and the antiserum was obtained by intramuscular injection of purified viral preparations in rabbit. Tomato and potato tissue samples were used to validate the polyclonal antiserum for detection of ToCV, previously analyzed by RT-PCR. A total of 81 tomato and potato samples were analyzed by RT-PCR and dot-blot immunoassay (DBIA). All samples were positive in RT-PCR, but three of them did not react in DBIA, showing of 96.3% efficiency compared with that in RT-PCR. All potato genotypes inoculated with ToCV by Bemisia tabaci MEAM1 were susceptible to infection. Potato plants of cv. Camila infected by ToCV showed the lowest virus titer and were asymptomatic.

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Acknowledgments

The Soleil Papa Tecnologia donated seed potatoes.

Funding

This study was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), grant number 2012/51771-4. The first and the second author obtained a scholarship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), grant numbers 140708/2014-6 and 153431/2018-0. The sixth author is a fellow of CNPq, grant number 302723/2019-6.

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Authors and Affiliations

  1. Departamento de Fitopatologia e Nematologia, ESALQ, Universidade de São Paulo, Piracicaba, São Paulo, 13418-900, Brazil

    Luiz R. Pinto, Daiana Bampi, Talita N. Z. Silva, Elliot W. Kitajima, Jorge A. M. Rezende & Armando Bergamin Filho

  2. Instituto Nacional de Tecnología Agropecuária, INTA, 7620, Balcarce, Argentina

    Juan P. Edwards Molina

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  1. Luiz R. Pinto
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  2. Daiana Bampi
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  3. Talita N. Z. Silva
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  4. Juan P. Edwards Molina
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  5. Elliot W. Kitajima
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  6. Jorge A. M. Rezende
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  7. Armando Bergamin Filho
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Correspondence to Daiana Bampi or Jorge A. M. Rezende.

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Pinto, L.R., Bampi, D., Silva, T.N.Z. et al. Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection. Trop. plant pathol. 46, 156–162 (2021). https://doi.org/10.1007/s40858-020-00379-0

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