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Latency and incubation of ‘Candidatus Liberibacter asiaticus’ in citrus after vector inoculation

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Abstract

Huanglongbing (HLB) is a bacterial disease of citrus, transmitted by the Asian citrus psyllid Diaphorina citri. In Brazil, the disease is managed by insecticide spray targeting vector control and removal of trees when they first show HLB symptoms that aims to reduce ‘Candidatus Liberibacter asiaticus’ (CLas) inoculum within the orchard. Albeit symptoms of HLB in field trees appear only months or years after the initial infection, the shortest incubation period (time between pathogen infection and symptom expression) reported for CLas in citrus was about 4 months after inoculation in studies in which this pathogen was graft-inoculated. In this work, an incubation period of 80 days (2.5 months) was recorded for citrus seedlings and 5 months for citrus nursery plants after inoculation with the psyllid vector itself. Psyllids were capable of acquire CLas from the infected seedlings and nursery plants 2.5 months after inoculation. These results show that the latency of CLas in planta is shorter than the incubation. Assessment of the presence of liberibacter in psyllids could be used as a proxy for the presence of HLB bacterium in presymptomatic stage of the plants.

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Acknowledgements

We thank the National Council for Scientific and Technological Development (CNPq, Brazil), for the scholarship to the second author and Daniela Sansoni for the English review.

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Authors and Affiliations

  1. Escola de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, 13418-900, Brazil

    Maria Cristina Canale, Karen M. A. Komada & João R. S. Lopes

  2. Empresa de Pesquisa Agropecuária e de Extensão Rural de Santa Catarina (EPAGRI), Chapecó, 89809-450, Brazil

    Maria Cristina Canale

Authors
  1. Maria Cristina Canale
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  2. Karen M. A. Komada
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  3. João R. S. Lopes
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Contributions

MCC, project initiation, performance of Experiment 1, data analysis and writing of manuscript; KMAK, maintenance of plants and insects, performance of Experiment 2 and data analysis; JRSL, project management (experimental working) and writing of manuscript.

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Correspondence to Maria Cristina Canale.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Section Editor: Alessandra A. de Souza

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Canale, M.C., Komada, K.M.A. & Lopes, J.R.S. Latency and incubation of ‘Candidatus Liberibacter asiaticus’ in citrus after vector inoculation. Trop. plant pathol. 45, 320–326 (2020). https://doi.org/10.1007/s40858-019-00311-1

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