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Tropical Plant Pathology

, Volume 43, Issue 3, pp 247–253 | Cite as

Identification of Colletotrichum species associated with brown spot of cactus prickly pear in Brazil

  • Lucas F. M. Oliveira
  • Frederico M. Feijó
  • Adso L. S. F. Mendes
  • Jose D. V. Neto
  • Mariote S. B. Netto
  • Iraildes P. Assunção
  • Gaus S. A. Lima
Short Communication

Abstract

Cactus prickly pear (Nopalea cochenillifera) is native to Mexico and is widespread throughout the world. In Brazil, this plant is cultivated exclusively in the Northeast region. Although presenting extreme rusticity and adaptability to the conditions of the Northeast, cactus prickly pear suffers from the attack of pests and diseases that can significantly reduce its productive potential. The objective of this study was to conduct a molecular and morphological characterization of Colletotrichum species associated with brown spot disease of cactus prickly pear. Cladodes showing symptoms of brown spot were collected in producing areas in the states of Alagoas and Pernambuco. The fungal isolates were identified molecularly using the GAPDH, TUB2 and ITS-rDNA genic regions, along with morphological characterization. Several isolates obtained from diseased tissue were identified as Colletotrichum: C. siamense, C. fructicola and C. karstii. To the best of our knowledge, this is the first report of the association of C. siamense, C. fructicola and C. karstii with brown spot in cactus prickly pear worldwide.

Keywords

Anthracnose Nopalea cochenillifera Polyphasic taxonomy 

References

  1. Bragança CAD, Silva LL, Haddad F, Oliveira SAS (2016) First report of Colletotrichum fructicola causing anthracnose in cassava (Manihot esculenta) in Brazil. Plant Disease 100:857CrossRefGoogle Scholar
  2. Capobiango NP, Pinho DB, Zambolim L, Pereira OL, Lopes UP (2015) Anthracnose on strawberry fruits caused by Colletotrichum siamense in Brazil. Plant Disease 100:859CrossRefGoogle Scholar
  3. Costa JFO, Ramos-Sobrinho R, Chaves TP, Silva JRA, Pinho DB, Assunção IP, Lima GSA (2017) First report of Colletotrichum fructicola causing anthracnose on annona leaves in Brazil. Plant Disease 101:386CrossRefGoogle Scholar
  4. Damm U, Cannon PF, Woudenberg JH, Johnston PR, Weir BS, Tan YP, Shivas RG, Crous PW (2012) The Colletotrichum boninense species complex. Studies in Mycology 73:1–36CrossRefPubMedPubMedCentralGoogle Scholar
  5. Damm U, O'Connell RJ, Groenewald JZ, Crous PW (2014) The Colletotrichum destructivum species complex hemibiotrophic pathogens of forage and field crops. Studies in Mycology 79:49–84CrossRefPubMedPubMedCentralGoogle Scholar
  6. Dean R, Van Kan JA, Pretorius ZA, Hammond-Kosack KE, Di Pietro A, Spanu PD, Rudd JJ, Dickman M, Kahmann R, Ellis J, Foster GD (2012) The top 10 fungal pathogens in molecular plant pathology. Molecular Plant Pathology 13:414–430CrossRefPubMedGoogle Scholar
  7. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry 19:11–15Google Scholar
  8. Dubeux Júnior JCB (2013) Potencial da palma forrageira na América do Sul. Cactus net Newsletter, Santiago del Estero 13:29–40Google Scholar
  9. Edgar RC (2004) Muscle: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5:1–19CrossRefGoogle Scholar
  10. Feijó FM, Ramos-Sobrinho R, Silva MJS, Barbosa LF, Pinho DB, Assunção IP, Lima GSA (2016) First report of cladode and foot rots caused by Pythium aphanidermatum on cactus (Nopalea cochenillifera). Plant Disease 100:797CrossRefGoogle Scholar
  11. Flores-Flores R, Valle MGV, León-Rodriguez R, Flores-Moctezuma HE, Hernández-Lauzardo AN (2013) Identification of fungal species associated with cladode spot of prickly pear and their sensitivity to chitosan. Journal of Phytopathology 161:544–552CrossRefGoogle Scholar
  12. Hoffmann W (2001) Etnobotânica. In: Agroecologia, cultivo e usos da palma forrageira. Roma/Brasilia: FAO/SEBRAE–PB. pp. 12–19Google Scholar
  13. Kim YH, Jun OK, Sung MJ, Jeong MI (2000) Occurrence of Colletotrichum stem rot caused by Glomerella cingulata on graft-cactus in Korea. Plant Pathology Journal 16:242–245Google Scholar
  14. Lima NB, Marques MW, Michereff SJ, Morais MA Jr, Barbosa MAG, Câmara MPS (2013) First report of mango anthracnose caused by Colletotrichum karstii in Brazil. Plant Disease 97:1248CrossRefGoogle Scholar
  15. Maia Neto AL (2003) Utilização da palma forrageira para produção de leite no semi-árido nordestino. Bahia Agrícola 5:45–49Google Scholar
  16. Méndez-Gallegos SJ, Talavera-Magaña D, Garcia-Herrera EJ (2008) Identificación y control de las principales enfermedades del nopal. Revista Salud Pública y Nutrición 2:2–13Google Scholar
  17. Menezes M (2006) Aspectos biológicos e taxonômicos de espécies do gênero Colletotrichum. Academia Pernambucana de Ciência Agronômica 3:170–179Google Scholar
  18. Posada D, Buckley TR (2004) Model selection and model averaging in phylogenetics: advantages of Akaike information criterion and Bayesian approaches over likelihood ratio tests. Systematic Biology 53:793–808CrossRefPubMedGoogle Scholar
  19. Prihastuti H, Cai L, Chen H, McKenzie EHC, Hyde KD (2009) Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Diversity 39:89–109Google Scholar
  20. Rannala B, Yang Z (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal of Molecular Evolution 43:304–311CrossRefPubMedGoogle Scholar
  21. Rios JA, Pinho DB, Moreira WR, Pereira OL, Rodrigues FA (2015) First report of Colletotrichum karstii causing anthracnose on blueberry leaves in Brazil. Plant Disease 99:157CrossRefGoogle Scholar
  22. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61:539–542CrossRefPubMedPubMedCentralGoogle Scholar
  23. Rosa RCT, Cavalcanti VALB, Coelho RSB, Guimarães LMP, Serra IMR, Bezerra BCC, Gurgel LMS (2008) Assinalamento de Colletotrichum gloeosporioides em palma forrageira cultivar doce (Nopalea cochenillifera Salm-Dyck). In: XLI Congresso Brasileiro de Fitopatologia, Belo Horizonte, SBFITO, p 220Google Scholar
  24. Santos DC (2006). Manejo e utilização da palma forrageira (Opuntia e Nopalea) em Pernambuco. Recife: IPA (IPA, Documento 30). 48pGoogle Scholar
  25. Sharma G, Pinnaka AK, Shenoy BD (2013) ITS-based diversity of Colletotrichum from India. Current Research in Environmental & Applied Mycology 3:194–220CrossRefGoogle Scholar
  26. Silva DN, Talhinhas P, Várzea V, Cai L, Paulo OS, Batista D (2012) Application of the Apn2/MAT locus to improve the systematics of the Colletotrichum gloeosporioides complex: an example from coffee (Coffea spp.) hosts. Mycologia 104:396–409CrossRefPubMedGoogle Scholar
  27. Silva JRA, Chaves TP, da Silva ARG, Barbosa LF, Costa JFO, Ramos-Sobrinho R, Teixeira RRO, Silva SJCS, Lima GSA, Assunção IP (2017) Molecular and morpho-cultural characterization of Colletotrichum spp. associated with anthracnose on Capsicum spp. in northeastern Brazil. Tropical Plant Pathology 42:315–319CrossRefGoogle Scholar
  28. Souza AEF, Nascimento LC, Araújo E, Lopes EB, Souto FM (2010) Ocorrência e identificação dos agentes etiológicos de doenças em palma forrageira (Opuntia fícus-indica Mill.) no semiárido paraibano. Revista Biotemas 23:11–20Google Scholar
  29. Sutton BC (1992) The genus Glomerella and its anamorph Colletotrichum. In: Bailey JA, Jeger MJ (eds) Colletotrichum: biology, pathology, and control. CAB International, Wallingford, pp 1–26Google Scholar
  30. Takahashi LM, Rosa DD, Basseto BMA, Souza HG, Furtado EL (2008) First report of Colletotrichum gloeosporioides on Hylocereus megalanthus in Brazil. Australasian Plant Disease Notes 3:96–97CrossRefGoogle Scholar
  31. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2013) MEGA 5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28:2731–2739CrossRefGoogle Scholar
  32. Vieira WAS, Michereff SJ, Morais MA Jr, Hyde KD, Câmara MPS (2014) Endophytic species of Colletotrichum associated with mango in northeastern Brazil. Fungal Diversity 67:181–202CrossRefGoogle Scholar
  33. Weir BS, Johnston PR, Damm U (2012) The Colletotrichum gloeosporioides species complex. Studies in Mycology 73:115–180CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Sociedade Brasileira de Fitopatologia 2018

Authors and Affiliations

  • Lucas F. M. Oliveira
    • 1
  • Frederico M. Feijó
    • 1
  • Adso L. S. F. Mendes
    • 1
  • Jose D. V. Neto
    • 1
  • Mariote S. B. Netto
    • 1
  • Iraildes P. Assunção
    • 1
  • Gaus S. A. Lima
    • 1
  1. 1.Setor de Fitossanidade/Centro de Ciências AgráriasUniversidade Federal de AlagoasRio LargoBrazil

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