Tropical Plant Pathology

, Volume 43, Issue 2, pp 165–169 | Cite as

Turnip leaf curl disease associated with two begomoviruses in south-eastern Iran

  • Jahangir Heydarnejad
  • Mehdi Kamali
  • Vahid Hassanvand
  • Hossain Massumi
  • Marzieh Shamshiri
  • Arvind Varsani
Short Communication


A handful of geminiviruses have been associated with turnip leaf curl disease. As part of our turnip surveys in Kerman province (south-eastern Iran), turnip samples showing mild leaf curl disease were collected and screened for turncurto- and begomoviruses by PCR. In four out of seven samples, we identified co-infection with begomoviruses of the species Tomato yellow leaf curl virus and Watermelon chlorotic stunt virus. Neither of these two begomovirus species have been described to be associated with turnip leaf curl disease in Iran. Using infectious clones and agroinoculation of turnip, tomato and watermelon seedlings, our results indicated that single and/or co-agroinoculation of plants with the two begomovirus constructs result in symptoms with different severity in turnip, tomato and watermelon.


Tomato yellow leaf curl virus Watermelon chlorotic stunt virus Antagonism Rolling circle amplification Synergism Turnip 



This research work was supported by the Iran National Science Foundation (INSF) (Grant No. 92012168).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.


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Copyright information

© Sociedade Brasileira de Fitopatologia 2017

Authors and Affiliations

  1. 1.Department of Plant Protection, College of AgricultureShahid Bahonar University of KermanKermanIran
  2. 2.The Biodesign Center of Fundamental and Applied Microbiomics, School of Life Sciences, Center for Evolution and MedicineArizona State UniversityTempeUSA
  3. 3.Structural Biology Research Unit, Department of Clinical Laboratory SciencesUniversity of Cape TownCape TownSouth Africa

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