Skip to main content
SpringerLink
Log in

Seasonal incidence of ‘Candidatus Liberibacter asiaticus’ (Rhizobiales: Rhizobiaceae) in Diaphorina citri (Hemiptera: Liviidae) in Colima, Mexico

  • Short Communication
  • Published:
Tropical Plant Pathology Aims and scope Submit manuscript

Abstract

By April 2016, 22 out of the 24 citrus-producing states in Mexico had confirmed the presence of ‘Candidatus Liberibacter asiaticus’ (C. Las) in citrus trees and in the vector Diaphorina citri. The dissemination of C. Las in space and time is the result of a complex interaction between the host, the associated pathogen, the vector and the environment. During 22 months (July 2012–April 2014), the seasonal variation of C. Las in D. citri populations was determined by real-time polymerase chain reaction (PCR) in lime orchards at six locations in two municipalities in the state of Colima, Mexico. Results showed that the proportion of positive psyllids was very high throughout the year (overall incidence of 58.2%) with seasonal variability in the proportion of positive adults, whereby winter and spring have the highest levels. These periods mark the co-occurrence of a high density of potentially infective psyllids on a susceptible host with continual generation of new shoots, and optimal environmental conditions in the Pacific region.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Aurambout JP, Finlay KJ, Luck J, Beattie GAC (2009) A concept model to estimate the potential distribution of the Asiatic citrus psyllid (Diaphorina citri Kuwayama) in Australia under climate change - a means for assessing biosecurity risk. Ecol Model 220:2512–2524

    Article  Google Scholar 

  • Bové JM (2006) Huanglongbing: a destructive, newly emerging, century-old disease of citrus. J Plant Pathol 88:7–37

    Google Scholar 

  • Capoor SP, Rao DG, Viswanath SM (1967) Diaphorina citri Kuwayama, a vector of greening disease of citrus in India. Indian J Agric Sci 37:572–576

    Google Scholar 

  • Da Graça JV (1991) Citrus greening disease. Annu Rev Phytopathol 29:109–136

    Article  Google Scholar 

  • Díaz-Padilla G, López-Arroyo JI, Sánchez-Cohen I, Guajardo-Panes RA, Mora-Aguilera G, Quijano-Carranza JA (2014) Áreas de abundancia potencial en México del vector del huanglongbing, Diaphorina citri (Hemiptera: Liviidae). Rev Mex Cienc Agric 5:1137–1153

    Google Scholar 

  • Flores-Sánchez JL, Mora-Aguilera G, Loeza-Kuk E, López-Arroyo JI, Domínguez-Monge S, Acevedo-Sánchez G, Robles-García P (2015) Pérdidas en producción inducidas por 'Candidatus Liberibacter asiaticus' en limón persa en Yucatán, México. Rev Mex Fitopatol 33:195–210

    Google Scholar 

  • Flores-Virgen R, Robles-González MM, Velázquez-Monreal JJ, Manzanilla-Ramírez MA (2010) Situación actual del huanglongbing (HLB) en limón mexicano bajo las condiciones agroecológicas de Colima. In: Primer Simposio Internacional sobre Mejoramiento Genético de Cítricos. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Mexico City. p. 1-20

  • Fung YC, Chen CN (2006) Effects of temperature and host plant on population parameters of the citrus psyllid (Diaphorina citri Kuwayama). Formos Entomol 26:190–123

  • Haapalainen M (2014) Biology and epidemics of 'Candidatus Liberibacter' species, psyllid transmitted plant-pathogenic bacteria. Ann Appl Biol 165:172–198

    Article  CAS  Google Scholar 

  • Hall DG, Richardson ML, Ammar E-D, Halbert SE (2012) Asian citrus psyllid, Diaphorina citri, vector of citrus huanglongbing disease. Entomol Exp Appl 146:207–223

    Article  Google Scholar 

  • Hoffman MT, Doud MS, Williams L, Zhang MQ, Ding F, Stover E, Hall D, Zhang S, Jones L, Gooch M, Fleites L, Dixon W, Gabriel D, Duan YP (2013) Heat treatment eliminates 'Candidatus Liberibacter asiaticus' from infected citrus trees under controlled conditions. Phytopathology 13:15–22

    Article  Google Scholar 

  • Keremane ML, Ramadugu C, Castaneda A, Díaz JE, Peñaranda EA, Chen J, Duan YP, Halbert SE, Lee RF (2015) Report of 'Candidatus Liberibacter caribbeanus', a new citrus- and psyllid-associated Liberibacter from Colombia, South America. (Abstr.) Phytopathology 105:S4.71

  • Li W, Hartung JS, Levy L (2006) Quantitative real-time PCR for detection and identification of 'Candidatus Liberibacter' species associated with citrus huanglongbing. J Microbiol Methods 66:104–115

    Article  CAS  PubMed  Google Scholar 

  • Li W, Duan Y, Brlansky RH, Twieg E, Levy L (2008) Incidence and population of 'Candidatus Liberibacter asiaticus' in Asian citrus psyllids (Diaphorina citri) on citrus plants affected by huanglongbing in Florida. In: International Research Conference on Huanglongbing, Proceedings. IRCHLB, Orlando. p. 261–264

  • Liu YH, Tsai HJ (2000) Effects of temperature on biology and life table parameters of Asian citrus psyllid, Diaphorina citri Kuwayama (Homoptera: Psyllidae). Ann Appl Biol 137:201–206

    Article  Google Scholar 

  • Lopes SA, Frare GF, Bertolini E, Cambra M, Fernandes NG, Ayres AJ, Marin DR, Bové JM (2009) Liberibacters associated with citrus huanglongbing in Brazil: 'Candidatus Liberibacter asiaticus' is heat tolerant, 'Ca. L. americanus' is heat sensitive. Plant Dis 93:257–262

    Article  Google Scholar 

  • Manjunath KL, Halbert SE, Ramadugu C, Webb S, Lee RF (2008) Detection of 'Candidatus Liberibacter asiaticus' in Diaphorina citri and its importance in the management of citrus huanglongbing in Florida. Phytopathology 98:387–396

    Article  CAS  PubMed  Google Scholar 

  • McLean APD, Oberholzer PCJ (1965) Citrus psylla, a vector of the greening disease of sweet orange. S Afr J Agric Sci 8:297–298

    Google Scholar 

  • Mora-Aguilera G, Robles-García P, Lopez-Arroyo JI, Flores-Sánchez J, Acevedo-Sánchez G, Domínguez-Monge S, Gutierrez-Espinosa A, Loeza-Kuk E (2014) Situación actual y perspectias del manejo del HLB de los cítricos. Rev Mex Fitopatol 32:108–119

    Google Scholar 

  • Nakata T (2006) Temperature-dependent development of the citrus psyllid, Diaphorina citri (Homoptera: Psylloidea), and the predicted limit of its spread based on overwintering in the nymphal stage in temperate regions of Japan. Appl Entomol Zool 41:383–387

  • NAPPO (2009) North American protection organization. Phytosanitary alert system: detection of Huanglongbing ('Candidatus Liberibacter asiaticus') in the municipallity of Tizimin, Yucatan, Mexico. Available at: http://www.pestalert.org. Accessed on 9 Dec 2015

  • Nava DE, Torres MLG, Rodrigues MDL, Bento JMS, Parra JRP (2007) Biology of Diaphorina citri (Hem., Psyllidae) on different hosts and at different temperatures. J Appl Entomol 131:709–715

  • Palomares-Pérez M, Córdoba-Urtiz EG, Sánchez-González JA, Medina-García NI, Hernández-Mendoza R, Pérez-Díaz VH, Arredondo-Bernal HC (2015) Aspectos biológicos de Diaphorina citri (Hemiptera: Liviidae) bajo condiciones no controladas en invernadero. Rev Col Entomol 41:228–234

    Google Scholar 

  • Razi MF, Keremane ML, Ramadugu C, Roose M, Khan IA, Lee RF (2014) Detection of citrus huanglongbing-associated ‘Candidatus Liberibacter asiaticus’ in citrus and Diaphorina citri in Pakistan, seasonal variability, and implications for disease management. Phytopathology 104:257–268

    Article  CAS  PubMed  Google Scholar 

  • Robles-González MM, Velázquez-Monreal JJ, Manzanilla-Ramírez MA, Orozco-Santos M, Medina-Urrutia VM, López-Arroyo JI, Flores-Virgen R (2013a) Síntomas del huanglongbing (HLB) en árboles de limón Mexicano [Citrus aurantifolia (Christm.) Swingle] y su dispersión en el estado de Colima, México. Rev Chapingo Ser Hort 19:15–31

    Article  Google Scholar 

  • Robles-González MM, Velázquez-Monreal JJ, Manzanilla-Ramírez MA, Orozco-Santos M, Medina-Urrutia VM (2013b) Experiencias sobre el huanglongbing (HLB) en limón Mexicano de Colima. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Mexico City

  • Sauer AV, Zanutto CA, Nocchi PTR, Machado MA, Bock CH, Nunes WMC (2015) Seasonal variation in populations of 'Candidatus Liberibacter asiaticus' in citrus trees in Paraná state, Brazil. Plant Dis 99:1125–1132

    Article  Google Scholar 

  • SENASICA (2010) Servicio Nacional de Sanidad Inocuidad y Calidad Agroalimentaria. Huanglongbing de los cítricos. Available at: http://publico.senasica.gob.mx/?id=4612. Accessed on 29 Oct 2015

  • SENASICA (2015) Servicio Nacional de Sanidad Inocuidad y Calidad Agroalimentaria Available at: http://publico.senasica.gob.mx/?id=4608. Accessed on 14 Sept 2015

  • Texeira DC, Ayres J, Kitajima EW, Danet L, Jagoueix-Eveillard S, Saillard C, Bové JM (2005) First report of a huanglongbing-like disease of citrus in Sao Paulo state, Brazil and association of a new Liberibacter species, 'Candidatus Liberibacter americanus', with the disease. Plant Dis 89:107–111

    Article  Google Scholar 

  • Velázquez-Monreal JJ, Robles-González MM, Manzanilla-Ramírez MA, Carrillo-Medrano SH, Orozco-Santos M (2011) Dinámica poblacional de Diaphorina citri Kuwayama (Hemiptera: Psillydae) en el estado de Colima. In: Segundo Simposio Nacional sobre investigación para el manejo del psílido asiatico de los cítricos y el huanglongbing en México, Proceedings... Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Mexico City. p. 161–168

  • Yang Y, Huang M, Beattie GA, Xia Y, Ouyang G, Xiong J (2006) Distribution, biology, ecology and control of the psyllid Diaphorina citri Kuwayama, a major pest of citrus: a status report for China. Int J Pest Manag 52:343–352

    Article  Google Scholar 

Download references

Acknowledgements

We thank Cecilia Moreno for her technical support in DNA extraction of a large number of samples.

Author information

Authors and Affiliations

  1. Centro Nacional de Referencia de Control Biológico, SENASICA-DGSV-CNRF 28110 Tecomán, Colima, Mexico

    Alba Suaste-Dzul, Adrien Gallou, Gabriel Moreno-Carrillo, Jorge Sánchez-González, Martín Palomares-Pérez & Hugo Arredondo-Bernal

  2. Facultad de Zootecnia y Ecología, Universidad Autónoma de Chihuahua, 33820, Chihuahua, Chihuahua, Mexico

    Monserrath Félix-Portillo

Authors
  1. Alba Suaste-Dzul
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Adrien Gallou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Monserrath Félix-Portillo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gabriel Moreno-Carrillo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jorge Sánchez-González
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Martín Palomares-Pérez
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hugo Arredondo-Bernal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adrien Gallou.

Additional information

Section Editor: Harald Scherm

Electronic supplementary material

ESM 1

(DOCX 19 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Suaste-Dzul, A., Gallou, A., Félix-Portillo, M. et al. Seasonal incidence of ‘Candidatus Liberibacter asiaticus’ (Rhizobiales: Rhizobiaceae) in Diaphorina citri (Hemiptera: Liviidae) in Colima, Mexico. Trop. plant pathol. 42, 410–415 (2017). https://doi.org/10.1007/s40858-017-0175-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40858-017-0175-z

Keywords

Search

Navigation