Abstract
The incidence of Groundnut rosette assistor virus (GRAV) in farmers’ fields and sequence diversity of groundnut rosette disease (GRD) agents were assessed in the three northern groundnut production regions of Ghana. GRAV incidence was high (69.5 to 75.0%) but not significantly different between the regions. Nucleotide sequencing of GRAV coat protein (CP) gene revealed 99–100% identity among the Ghanaian isolates and 97–100% similarity to GRAV sequences from Nigeria and Malawi for both nucleotide and predicted amino acids. Nucleotide sequence identities of partial ORF3 and 4 of Groundnut rosette virus (GRV) among the Ghanaian isolates were more variable (89–100%). Ghanaian GRV isolates were more closely related in nucleotide sequence identity to Nigerian isolates (95–98%) than Malawian isolates (88–90%). Similarly, nucleotide identity within Ghanaian GRV-sat RNA’s were close (94–100%), but distinct from Nigerian (82–87%) and Malawian (82–86%) GRV-sat RNAs. Ghanaian isolates of all three agents of GRD showed no obvious isolate diversity patterns based on the regions from where they were collected. We present the first report on the distribution and genetic diversity of GRD agents in Ghana.
Similar content being viewed by others
References
Adu-Dapaah HK, Asibuo JY, Danquah OA, Asumadu H, Haleegoah J, Agyei BA (2004) Farmer participation in groundnut rosette resistant varietal selection in Ghana. In: Ahmed KZ (ed) 8th African Crop Science Society Conference, El-Minia, Egypt, p 1435–1439
Altschul SF, Gish W, Miller W, Myers EW, Lipmon DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Appiah AS, Offei SK, Tegg RS, Wilson CR (2016) Varietal response to groundnut rosette disease and the first report of Groundnut ringspot virus in Ghana. Plant Dis 100:946–952
Blok VC, Ziegler A, Scott K, Dangora DB, Robinson DJ, Murant AF (1995) Detection of groundnut rosette umbravirus infections with radioactive and non-radioactive probes to its satellite RNA. Ann Appl Biol 127:321–328
Bock K, Murant A, Rajeshwari R (1990) The nature of the resistance in groundnut to rosette disease. Ann Appl Biol 117:379–384
Casper R, Meyer S, Lesemann DE, Reddy DVR, Rajeshwari R, Misari SM, Subbarayadu SS (1983) Detection of a luteovirus in groundnut rosette diseased groundnuts (Arachis hypogaea) by enzyme-linked immonosorbent assay and immunoelectron microscopy. Phytopathol Z 108:12–17
Chiyembekeza AJ, Subrahmanyam P, Hildebrand GL (1997) Identification and farm evaluation of rosette-resistant groundnut genotypes in Malawi. In: Reddy DVR, Delfosse P, Lenne JM, Subrahmanyam P (eds) Groundnut virus diseases in Africa: summary and recommendations. Sixth meeting of the international working group, Agricultural Research Council, Plant Protection Research Institute, Pretoria, South Africa. International Crops Research Institute for the Semi-Arid Tropics, Andhra Pradesh, and Belgian Administration for Development Cooperation, Brussells, p 20–21
Deom CM, Naidu RA, Chiyembekeza AJ, Ntare BR, Subrahmanyam P (2000) Sequence diversity within the three agents of groundnut rosette disease. Phytopathology 90:214–219
Gerlach WL, Llewellyn D, Haseloff J (1987) Construction of a plant disease resistance gene from the satellite RNA of tobacco ringspot virus. Nature 328:802–805
Gibbons RW (1977) Groundnut rosette virus. In: Kranz J, Schutter J, Koch W (eds) Diseases of tropical crops. Verlag Paul Parey, Berlin, pp 19–21
Harrison BD, Mayo MA, Baulcombe DC (1987) Virus resistance in transgenic plants that express Cucumber mosaic virus satellite RNA. Nature 328:799–801
Hull R (1964) Spread of Groundnut rosette virus by Aphis craccivora (Koch). Nature 202:213–214
Hull R (2002) Mathew’s plant virology, 4th edn. Elsevier Academic Press, New York
Kawchuk LM, Lynch DR, Martin RR, Kozub GC, Farries B (1997) Field resistance to the Potato leafroll luteovirus in transgenic and somaclone potato plants reduces tuber disease symptoms. Can J Plant Pathol 19:260–266
Kim SJ, Lee SJ, Kim B-D, Paek K-H (1997) Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. Golden Tower). Plant Cell Rep 16:825–830
Lizarraga C, Fernandez-Northcote EN (1989) Detection of potato viruses X and Y in sap extracts by a modified indirect enzyme-linked immunosorbent assay on nitrocellulose membranes (NCM-ELISA). Plant Dis 73:11–14
Lomonossoff GP (1995) Pathogen-derived resistance to plant viruses. Annu Rev Phytopathol 33:323–343
Murant AF (1990) Dependence of groundnut rosette virus on its satellite RNA as well as on groundnut rosette assistor luteovirus for transmission by Aphis craccivora. J Gen Virol 71:2163–2166
Murant AF, Kumar IK (1990) Different variants of the satellite RNA of groundnut rosette virus are responsible for the chlorotic and green forms of groundnut rosette disease. Ann Appl Biol 117:85–92
Murant AF, Rajeshwari R, Robinson DJ, Raschke JH (1988) A satellite RNA of groundnut rosette virus that is largely responsible for symptoms of groundnut rosette disease. J Gen Virol 69:1479–1486
Murant AF, Robinson DJ, Gibbs MJ (1995) Genus Umbravirus. In: Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, Martelli GP, Mayo MA, Summers MD (eds) Virus taxonomy - classification and nomenclature of viruses. Sixth report of the international committee on the taxonomy of viruses. Springer, Vienna, pp 388–391
Naidu RA, Bottenberg H, Subrahmanyam P, Kimmins FM, Robinson DJ, Thresh, JM. (1998) Epidemiology of groundnut rosette virus disease: current status and future research needs. Ann Appl Biol 132:525–548
Naidu RA, Kimmins FM, Deom CM, Subrahmanyam P, Chiyembekeza AJ, van der Merwe PJA (1999a) Groundnut rosette: a virus disease affecting groundnut production in sub-Saharan Africa. Plant Dis 83:700–709
Naidu RA, Kimmins FM, Holt J, Robinson DJ, Deom CM, Subrahmanyam P (1999b) Spatiotemporal separation of groundnut rosette disease agents. Phytopathology 89:934–941
Olorunju PE, Ntare BR, Pande S, Reddy SV (2001) Additional sources of resistance to groundnut rosette disease in groundnut germplasm and breeding lines. Ann Appl Biol 139:259–268
Page RD (1996) TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
Palukaitis P, Roossinck MJ (1996) Spontaneous change of a benign satellite RNA of Cucumber mosaic virus to a pathogenic variant. Nat Biotechnol 14:1264–1268
Qu J, Ye J, Fang R (2007) Artificial microRNA-mediated virus resistance in plants. J Virol 81:6690–6699
Reddy DVR, Murant AF, Duncan GH, Ansa OA, Demski JW, Kuhn CW (1985) Viruses associated with chlorotic rosette and green rosette diseases of groundnut in Nigeria. Ann Appl Biol 107:57–64
Storey HH, Ryland AK (1955) Transmission of Groundnut rosette virus. Ann Appl Biol 43:423–432
Subrahmanyam P, Hildebrand GL, Naidu RA, Reddy LJ, Singh AK (1998) Sources of resistance to groundnut rosette disease in global groundnut germplasm. Ann Appl Biol 132:473–485
Subrahmanyam P, Naidu RA, Reddy LJ, Lava Kumar P, Ferguson ME (2001) Resistance to groundnut rosette disease in wild Arachis species. Ann Appl Biol 139:45–50
Taliansky ME, Robinson DJ (1997) Trans-acting untranslated elements of groundnut rosette virus satellite RNA are involved in symptom production. J Gen Virol 78:1277–1285
Taliansky ME, Robinson DJ, Murant AF (1996) Complete nucleotide sequence and organization of the RNA genome of groundnut rosette umbravirus. J Gen Virol 77:2335–2345
Taliansky M, Roberts IM, Kalanina N, Ryabov EV, Raj SK, Robinson DJ, Oparka KJ (2003) An umbraviral protein involved in long-distance RNA movement binds viral RNA and forms unique, protective ribonucleoprotein complexes. J Virol 77:3031–3040
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position- specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Tien P, Zhang X, Qiu B, Qin B, Wu G (1987) Satellite RNA for the control of plant diseases caused by cucumber mosaic virus. Ann Appl Biol 111:143–152
Tsigbey FK, Brandenburg RL, Clottey VA (2003) Peanut production methods in northern Ghana and some disease perspectives. Available at: http://crsps.net/wpcontent/downloads/Peanut/Inventoried%208.27/7-2003-4-1122.pdf Accessed 25th June 2016
van der Wilk F, Willink DP-L, Huisman MJ, Huttinga H, Goldbach R (1991) Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection. Plant Mol Biol 17:431–439
Waliyar F, Kumar PL, Ntare BR, Monyo E, Nigam SN, Reddy AS, Osiru M, Diallo AT (2007) A century of research on groundnut rosette disease and its management. Information Bulletin no. 75. Patancheru 502 324 Andhra Pradesh, India: International Crops Research Institute for the Semi-Arid Tropics. 40pp
Wangai AW, Pappu SS, Pappu HR, Deom CM, Naidu RA (2001) Distribution and characteristics of groundnut rosette disease in Kenya. Plant Dis 85:470–474
Yayock JY, Rossel HW, Harkness C (1976) A review of the 1975 groundnut rosette epidemic in Nigeria. Inst. Agric. Res. Samaru Conf. Pap. No. 9. Institute of Agricultural Research, Samaru
Zimmermann G (1907) Über eine Krankheit der Erdnüsse (Arachis hypogaea). Der Pflanzer 3:129–133
Acknowledgements
We are grateful to the Tasmanian Institute of Agriculture and Biotechnology and Nuclear Agriculture Research Institute of the Ghana Atomic Energy Commission for the use of their facilities.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Funding information
The study was supported by a scholarship grant from Australian Aid for International Development for African Development to Andrew S. Appiah.
Conflict of interest
All authors declare they have no conflicts of interest.
Ethical approvals
This article does not contain any studies with human participants or animals performed by any of the authors.
Additional information
Section Editor: Michael Goodin
Rights and permissions
About this article
Cite this article
Appiah, A.S., Sossah, F.L., Tegg, R.S. et al. Assessing sequence diversity of Groundnut rosette disease agents and the distribution of Groundnut rosette assistor virus in major groundnut-producing regions of Ghana. Trop. plant pathol. 42, 109–120 (2017). https://doi.org/10.1007/s40858-017-0140-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s40858-017-0140-x