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Silk Fibroin Based Core-Shell Nanofibers Loaded with ZnO Nanoparticles: An Ideal Candidate for Designing a Medicated Wound Dressing

Journal of Medical and Biological Engineering (2023)Cite this article

Abstract

Purpose

Efficient addressing of injuries and wounds is one of the major demands of global healthcare systems. In the present study, we optimized multiple factors relevant to the coaxial electrospinning method to fabricate a novel and cost-effective core-shell nanofibrous scaffold consisting of silk fibroin as the shell and sodium alginate loaded with zinc oxide nanoparticles (ZnO-NPs) as the core.

Methods

We confirmed the successful configuration of core-shell nanofibers and even ZnO encapsulation within the core using transmission electron microscopy, energy-dispersive X-ray, and Fourier transform-infrared spectroscopy. Incorporation of ZnO-NPs into the core of electrospun nanofibers enabled sustained drug release and preserved its bioactivity.

Results

Antimicrobial evaluation of core-shell nanofibers showed their appropriate antibacterial activity against the gram-positive (Staphylococcus aureus) and the gram-negative (Escherichia coli) bacteria. However, E. coli was more resistant than S. aureus to ZnO-NPs. Additionally, the cytotoxicity and cell adhesion analyses suggested the appropriate cell attachment, viability, and proliferation on the ZnO-loaded core-shell nanofibers, which are fundamental for wound healing and skin regeneration.

Conclusion

Considering the used materials and our promising results, the synthesized nanofibrous scaffold would be efficient and affordable for mass production and clinical usage for designing a medicated wound dressing.

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Data Availability

The raw/processed data are available on reasonable request.

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Acknowledgements

The authors are grateful for the support from the Tehran University of Medical Sciences (TUMS). Research Council and lab contributions from the Skin and Stem cell Research Center of TUMS.

Funding

This work was partially supported by Skin and Stem cell Research Center, Tehran University of Medical Sciences (Grant No. 97-03-150-39275).

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Authors and Affiliations

  1. Skin and Stem cell Research Center (SSRC), Tehran University of Medical Sciences (TUMS), Tehran, Iran

    Sohrab Moradi & Mohammad Ali Nilforoushzadeh

  2. Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran

    Sohrab Moradi, Shahram Nazarian & Jamil Zargan

  3. Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran

    Peiman Brouki Milan

Authors
  1. Sohrab Moradi
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  2. Shahram Nazarian
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  3. Peiman Brouki Milan
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  4. Mohammad Ali Nilforoushzadeh
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  5. Jamil Zargan
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, analysis, and manuscript writing were performed by S.M. Sh.N, P.BM, and MA.N supervised the project and edited the manuscript.

Corresponding authors

Correspondence to Shahram Nazarian or Peiman Brouki Milan.

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The authors have no relevant financial or non-financial interests to disclose.

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This is an observational study. The Tehran University of Medical Sciences Research Ethics Committee has confirmed that no ethical approval is required.

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Moradi, S., Nazarian, S., Milan, P.B. et al. Silk Fibroin Based Core-Shell Nanofibers Loaded with ZnO Nanoparticles: An Ideal Candidate for Designing a Medicated Wound Dressing. J. Med. Biol. Eng. (2023). https://doi.org/10.1007/s40846-023-00821-z

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  • DOI: https://doi.org/10.1007/s40846-023-00821-z

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