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Morphological Post-implantation Features of Aortic Conduits After Long-term wet Storage



Improved methods for obtaining connective tissue structures in cardiac surgery are actively developed over the world. An important aspect of this research pertains to long-term wet storage of cardiovascular biomaterial that optimally preserves the initial morphological characteristics.


Native and decellularized porcine aortas were stored for 50 days in several biocidal solutions (complex alcohol solution, a mixture of ethanol and glycerol, a mixture of antibiotics), and subsequently implanted in animals and studied histologically. The aortic specimens were implanted subcutaneously into 15 adult WAG rats. The samples were retrieved after 3 months and evaluated for calcium content using a quantitative spectroanalytical procedure. Aortic specimens were also orthotopically implanted into the vascular bed of 3 minipigs, harvested after 6 months, and histologically evaluated.


Atomic absorption spectroscopy showed that decellularized materials accumulated 4.8–9.2 times less calcium, depending on the storage solution used. Morphological and morphometrical analysis of orthotopically implanted aortic fragments in minipigs showed better preservation of decellularized material that had been stored in a complex alcohol solution and mixture of antibiotics.


Functional and histological evidence was obtained demonstrating long-term storage without freezing of o aortic specimens without alteration of their biophysical properties and structure. The developed technique will be used in the future for the fabrication of vascular prostheses and heart valves.

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This work was carried out within the state assignment of Ministry of Health of Russian Federation (theme # 121031300224-1).

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Authors and Affiliations



All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by DS, MV and EK. MZ, YR. and EC. conducted animal experiments. DS analysed data and wrote the manuscript. IZ helped material preparation and analysis with constructive discussions All authors read and approved the final manuscript.

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Correspondence to David Sergeevichev.

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The authors declare no competing interests regarding this manuscript.

Ethical Approval

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the local ethical committee of NMRC named after academician E.N. Meshalkin of the Ministry of Health of the Russian Federation (approval date 26 Dec 2014, protocol 45).

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Sergeevichev, D., Vasiliyeva, M., Kuznetsova, E. et al. Morphological Post-implantation Features of Aortic Conduits After Long-term wet Storage. J. Med. Biol. Eng. (2023).

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  • Tissue mineralization
  • Xenograft
  • Cardiac prosthesis
  • Decellularization
  • Antimicrobial solution
  • Connective tissue