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Metabolites of Scutellariae Radix Inhibit Injury of Endothelial Cells in Hypoxia Device

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Abstract

The effects of the metabolites of Scutellariae Radix (SR), a traditional anti-inflammatory oriental herbal medicine, on hypoxia/reoxygenation (H/R)-induced inflammation and apoptosis of human umbilical vein endothelial cells (HUVECs) were investigated. SR metabolites (SRMs) were prepared from the sulfatase/β-glucuronidase-added serum of SR-decoction-fed rats and examined using high-performance liquid chromatography. HUVECs were put into a mechanical device conditioned with hypoxia (94 % N2, 5 % CO2, and 1 % O2) for 4 h (H4), and then reoxygenation (room air with 5 % CO2) for 4 h (R4), alone or in the presence of SRMs or vitamin C. Cell adhesion and apoptosis were detected using the enzyme-linked immunosorbent assay. Intercellular adhesion molecule-1 (ICAM-1), cyclooxygenase-2 (COX-2), caspase-3, and heme oxygenase-1 (HO-1) were evaluated using western blotting. COX-2 small hairpin RNA (shRNA) and HO-1 shRNA were used to evaluate the relationship of COX-2 and HO-1 to cell apoptosis. THP-1 adhesion, and ICAM-1 and COX-2 expression increased at H4 and H4/R4, but decreased at H8, and could be ameliorated by SRMs in a dose-dependent manner. Notedly, SRMs effectively attenuated COX-2-mediated cell apoptosis, which was enhanced by HO-1 shRNA, especially at H4/R4 injury. In conclusion, in a hypoxia chamber that mimics the hypoxic condition in acute coronary syndrome, SRMs protected H/R-induced inflammation and the death of endothelial cells via oxidative stress reduction.

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Acknowledgments

This work was supported by Grants from National Taiwan University Hospital Hsin-Chu Branch (HCH101-23) and Taoyuan General Hospital, Ministry of Health and Welfare, Executive Yuan (PTH10112).

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    Authors and Affiliations

    1. Division of Cardiology, Department of Internal Medicine, National Taiwan University College of Medicine and National Taiwan University Hospital Hsin-Chu Branch, Hsinchu, 30059, Taiwan

      Chia-Lun Chao

    2. School of Pharmacy, China Medical University, Taichung, 40402, Taiwan

      Shiuan-Pey Lin, Yu-Chi Hou & Pei-Dawn Lee Chao

    3. Department of Medical Research, China Medical University Hospital, Taichung, 40402, Taiwan

      Yu-Chi Hou

    4. Department of Internal Medicine, School of Medicine, Taipei Medical University, Taipei, 11031, Taiwan

      Nen-Chung Chang & Feng-Ming Ho

    5. Department of Neurology, Taoyuan General Hospital, Ministry of Health and Welfare, Executive Yuan, Taoyuan, 33004, Taiwan

      Yu-Ching Huang

    6. Division of Cardiology, Department of Internal Medicine, Taoyuan General Hospital, Ministry of Health and Welfare, Executive Yuan, 1492 Chung-Shan Road, Taoyuan, 33004, Taiwan

      Feng-Ming Ho

    7. Department of Biomedical Engineering, Chung Yuan Christian University, Taoyuan, 32023, Taiwan

      Feng-Ming Ho

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    Chia-Lun Chao and Shiuan-Pey Lin have contributed equally to this work.

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    Chao, CL., Lin, SP., Hou, YC. et al. Metabolites of Scutellariae Radix Inhibit Injury of Endothelial Cells in Hypoxia Device. J. Med. Biol. Eng. 35, 492–499 (2015). https://doi.org/10.1007/s40846-015-0057-0

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