Effect of Low-Intensity Ultrasound on Mortality of PC12 Induced by Amyloid β25–35
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The aim of this study is to investigate the potential of using ultrasound (US) to protect neuronal cells from damage by amyloid beta (Αβ) peptide. Experiments were performed using PC12 cells with the addition of 20 µM Aβ25–35, US stimulation, or both. 1-MHz US at a 20 % duty cycle with various intensities (10, 50, 10, and 150 mW/cm2) for 3 min was employed. The responses of PC12 cells were determined in terms of the survival rate via the MTT assay, cell morphology via optical microscopy, and cell apoptosis/necrosis characteristics via Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) fluorescence staining and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end-labeling assay. The results show that the survival rate of PC12 cells in the presence of Aβ23–35 increased by 4.8–6 % when the cells were exposed to 100- and 150-mW/cm2 US. With US intensity of 150 mW/cm2, the growth of neurites from PC12 cells was observed. The experiment results of cell stains with Annexin V-FITC/PI show that US decreased PC12 cell apoptosis and necrosis. In addition, for the PC12 cells with added Aβ25–35, the fold change in cell apoptosis decreased by 0.36 when a US intensity of 150 mW/cm2 was applied. In conclusion, low-intensity US decreases PC12 cell mortality caused by Aβ23–35.
KeywordsAmyloid beta peptide PC12 cells Low-intensity ultrasound
The authors would like to thank the Ministry of Science and Technology of the Republic of China for partially financially supporting this research under Grant National Science Council 94-2213-E-033-039.
- 13.Lim, G. P., Yang, F., Chu, T., Chen, P., Beech, W., Teter, B., et al. (2000). Ibuprofen suppresses plaque pathology and inflammation in a mouse model for Alzheimer’s disease. Journal of Neuroscience, 20, 5709–5714.Google Scholar
- 17.Grundman, M. (2000). Vitamin E and Alzheimer disease: the basis for additional clinical trials. The American Journal of Clinical Nutrition, 71, 630S–636S.Google Scholar
- 32.Chen, S., Wu, C., Wang. S., & Li, W. (2014). Growth and differentiation of osteoblasts regulated by low-intensity pulsed ultrasound of various exposure durations. Journal of Medical and Biological Engineering, 34, 197–203.Google Scholar
- 34.Breuing, K., Bayer, L., Neuwalder, J., & Orgill, D., (2005). Early experience using low-frequency ultrasound in chronic wounds. Annals of Plastic Surgery, 55, 183–187.Google Scholar
- 35.De Deyne, P. G., & Kirsch-Volders, M. (1995). In vitro effects of therapeutic ultrasound on the nucleus of human fibroblasts. Physical Therapy, 75, 629–634.Google Scholar
- 41.Ashush, H., Rozenszajn, L. A., Blass, M., Barda-Saad, M., Azimov, D., Radnay, J., et al. (2000). Apoptosis induction of human myeloid leukemic cells by ultrasound exposure. Cancer Research, 60, 1014–1020.Google Scholar
- 42.Firestein, F., Rozenszajn, L. A., Shemesh-Darvish, L., Elimelech, R., Radnay, J., & Rosenschein, U. (1010). Induction of apoptosis by ultrasound application in human malignant lymphoid cells: role of mitochondria-caspase pathway activation. Annals of the New York Academy of Sciences, 163–166, 2003.Google Scholar