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Utilization of Industrial Waste for the Production of Cellulase by the Cultivation of Trichoderma via Solid State Fermentation

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Abstract

The present study deals with the cultivation of Trichoderma sp. for the production of cellulase in lignocellulosic materials. The highest cellulase activity was determined under optimal conditions of pH (5.0), incubation temperature (30 °C), inocula concentration (2 x 108 spores mL-1) and particle size (500 μm). Maximum activity for raw palm kernel cake (PKC), defatted PKC, and vegetable waste (VW) substrates were achieved as 6.9 FPU g−1, 16.1 FPU g−1 and 50.1 FPU g−1 correspondingly. It was observed that defatted PKC served as a better substrate than raw PKC for cellulase activity. A comparative study for the production of enzymes via solid state fermentation (SSF) indicated that cellulase activity produced by Trichoderma was about 1-fold higher in PKCs. However, Bacillus cereus scored 2-folds higher activity than VW substrates. On the basis of the significant yield of cellulase activity, palm oil industry wastes can be successfully used for the production of cellulase. Thus, environmental pollution can be controlled by utilizing palm oil industrial wastes to generate value-added product (cellulase).

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Acknowledgments

This work was supported by the research university grant of University Sains Malaysia (No.1001/PJJAUH/811198). Special thanks to United Fleet Palm Sdn. Bhd. and School of Industrial Technology for the materials and facilities provided for this research.

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Authors and Affiliations

  1. School of Industrial Technology, University Sains Malaysia, 11800 Minden, Penang, Malaysia

    Tengku Norsalwani Tuan Lah & A. K. Mohd Omar

  2. School of Distance Education, University Sains Malaysia, 11800 Minden, Penang, Malaysia

    Nik Ab Rahman Nik Norulaini & Mohammad Shahadat

  3. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology IIT Delhi, Hauz Khas, New Delhi, -110016, India

    Mohammad Shahadat

  4. School of Biological Sciences, University Sains Malaysia, 11800 Minden, Penang, Malaysia

    Hideyuki Nagao

  5. Malaysian Institute of Chemical and Engineering Technology, Universiti Kuala Lumpur, 78000, Alor Gajah, Malacca, Malaysia

    Md Sohrab Hossain

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  1. Tengku Norsalwani Tuan Lah
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Correspondence to Nik Ab Rahman Nik Norulaini or Mohammad Shahadat.

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Lah, T.N.T., Norulaini, N.A.N., Shahadat, M. et al. Utilization of Industrial Waste for the Production of Cellulase by the Cultivation of Trichoderma via Solid State Fermentation. Environ. Process. 3, 803–814 (2016). https://doi.org/10.1007/s40710-016-0185-8

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