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High pan-allergen content in mango and peach in Taiwan

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Abstract

Background

Many plant food allergens have been implicated in cross-reactivities and may cause repetitive pan-allergenic exposures, which increase the severity of patients’ allergic symptoms. Identification of allergenic protein levels in food allows for more precise dietary advice. This study aimed to determine LTP, profilin, and PR-10 in the 15 most popular fruits in Taiwan.

Methods

cDNA encoding profilin (Sola l 1), nonspecific lipid transfer protein (nsLTP, Sola l 3), and pathogenesis-related protein (PR-10, Sola l 4) were cloned into the pQE30, expressed in Escherichia coli, and purified. Extracts of the most popular fruits, including apple, banana, carrot, celery, dragon fruit, hami melon, jujube, kiwi, mango, melon, orange, papaya, peach, persimmon, and tomato, in Taiwan were prepared in PBS. The allergenic protein levels in these extracts were determined by immuno-dot blotting using rabbit anti-rSola l 1, 3, and 4 polyclonal antibodies.

Results

Among all 15 fruit extracts tested, peach and mango contained the highest levels of profilin, nsLTP, and PR-10. Papaya, melon, and tomato contained low but detectable levels of the three pan-allergens.

Conclusion

Our data suggest that peach and mango contain the highest levels of important pan-allergens among the 15 common fruits tested, which agrees with epidemiological data.

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Abbreviations

AEC:

3-amino-9-ethylcarbazole

LTP:

Lipid transfer proteins

MW:

Molecular weight

NC:

Nitrocellulose membrane

nsLTP:

Nonspecific lipid transfer protein

PMSF:

Phenylmethyl sulfonyl fluoride

PR:

Pathogenesis-related

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Acknowledgements

This research work was supported by grants from Taichung Veterans General Hospital (TCVGH-1057318C and TCVGH-1067317C).

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Authors and Affiliations

  1. Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan

    Mey Fann Lee PhD

  2. Division of Allergy, Immunology, and Rheumatology, Taichung Veterans General Hospital, Taichung, Taiwan

    Yi Hsing Chen MD, PhD

  3. Department of Pharmacy, Taichung Veterans General Hospital, Taichung, Taiwan

    Yu-Wen Chu PhD

  4. Department of Biology, National Changhua University of Education, No. 1 Jin-der Road, 50050, Changhua, Taiwan

    Ping Cheng Hsieh MS & Nancy M. Wang PhD

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Correspondence to Nancy M. Wang PhD.

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Conflict of interest

M.F. Lee, Y.H. Chen, Y.-W. Chu, P.C. Hsieh and N.M. Wang declare that they have no competing interests.

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Lee, M.F., Chen, Y.H., Chu, YW. et al. High pan-allergen content in mango and peach in Taiwan. Allergo J Int 32, 97–103 (2023). https://doi.org/10.1007/s40629-022-00240-7

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