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Endophytic infection modulates ROS-scavenging systems and modifies cadmium distribution in rice seedlings exposed to cadmium stress

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Abstract

The relationship between endophytic infection and components of the Cd stress response was examined in the present study. Oryza sativa L. plants were grown in Hoagland’s nutrient solution with and without infection with the endophytic fungus JP4 under different cadmium (Cd) stress conditions (0, 50, 100, and 150 μM) for 2 weeks. Endophytic infection in the roots promoted rice seedling growth and decreased shoot Cd contents in comparison to non-infected treatments. Furthermore, the adsorbed Cd remained in the roots, and far less Cd was transported from the roots to the shoots. Compared to the non-infected rice seedlings, endophytic infection remarkably increased the pigment content, net photosynthetic rate (Pn), and chlorophyll fluorescence in all treatments, except for Fv/F0 values and chlorophyll b content in the 100 μM Cd treatment. Superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and glutathione reductase (GR) activity, as well as ascorbic acid (AsA) and glutathione (GSH) content, increased in EI seedlings relative to EF seedlings under Cd stress, while malondialdehyde (MDA) and H2O2 content decreased significantly. Inoculation of endophytic fungus JP4 in roots promoted rice seedling growth and ameliorated the negative effects of Cd stress on the growth of rice seedlings by triggering antioxidant systems and non-enzymatic systems, regulating photosynthetic activities and modifying the Cd distribution.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (31600314), and the Shenyang City Science and Technology Plan (F16-205-1-50), Major incubating project of Shenyang Normal University (ZD201705), Liaoning Province Science and Technology Plan Progect (No. 2017208001) and the Liaoning Province Natural Science Foundation (2015020762).

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  1. College of Life Science, Shenyang Normal University, Shenyang, 110034, People’s Republic of China

    Lianju Ma, Xuemei Li, Lanlan Wang, Yueying Li & Ning Bu

  2. College of Agronomy, Shenyang Agricultural University, Shenyang, 110161, People’s Republic of China

    Cuimei Yu

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  1. Lianju Ma
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Ma, L., Li, X., Wang, L. et al. Endophytic infection modulates ROS-scavenging systems and modifies cadmium distribution in rice seedlings exposed to cadmium stress. Theor. Exp. Plant Physiol. 31, 463–474 (2019). https://doi.org/10.1007/s40626-019-00159-5

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