Photochemistry of thylakoid membranes in two pea cultivars with different leaf colouration
- 190 Downloads
Photochemical features of thylakoid membranes and chlorophyll content were investigated in two pea (Pisum sativum L.) cultivars with different leaf colouration: ‘Assas’ (red leaves) and ‘Arvika’ (green leaves). In vivo measured chlorophyll fluorescence (OJIP) was used to evaluate overall photosynthetic efficiency (expressed as performance index, PIABS) as well as for advanced analysis of main photochemical processes per active reaction centre (RC) of photosystem II (PSII). To evaluate the response of PSII driven linear electron transport (relETR) and non-photochemical quenching (NPQ), the plants were challenged with short-term high light (~2000 µmol m−2 s−1). In spite of prevailing red colour, leaves of ‘Assas’ had identical concentrations of chlorophylls a and b as green ones. The OJIP transients showed that red leaves grown in field (500–800 µmol m−2 s−1) had decreased PIABS and electron transport per RC beyond primary acceptor (Q A − ) compared to green leaves. After high light exposure red leaves revealed impaired relETR accompanied with increased NPQ values. Anthocyanins located in epidermal cells affected neither chlorophylls concentrations nor the light capture features of PSII. Despite equal concentrations of chlorophylls and PSII photochemistry further than Q A − in both leaf types, red leaves reduced overall photosynthetic efficiency due to impaired relETR in thylakoid membranes.
KeywordsPisum sativum L. Anthocyanins Chlorophyll fluorescence Photosynthesis Photoprotection
Financial support for the work was provided by the Croatian Ministry of Science, Education and Sport (Grants 073-0731674-1673 to HL, 073-0000000-3535 to SP and 073-0731674-0841 to VC).
- Schreiber U, Bilger W, Neubauer C (1994) Chlorophyll fluorescence as a nonintrusive indicator of rapid assessment of in vivo photosynthesis. In: Schulze ED, Caldwell MM (eds) Ecophysiology of photosynthesis: ecological studies. Springer, Berlin, pp 49–70Google Scholar
- Štolfa I, Lepeduš H, Cesar V, Ljubešić N (2006) Leaf anatomy and photosynthetic apparatus functioning of red and green laeves from single tre of “Crimson King” Norway maple. In: Gajović S (ed) Proceedings of 2nd Croatian Congress on Microscopy with International Participation, Topusko, Croatia, 18–21 May 2006. Hrvatsko društvo za elektronsku mikroskopiju, Zagreb, pp 212–213Google Scholar
- Strasser RJ, Srivastava A, Tsimilli-Michael M (2000) The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Yunus M, Pathre U, Mohanty P (eds) Probing photosynthesis: mechanism, regulation & adaptation, 1st edn. CRC, New York, pp 445–483Google Scholar
- Tanino KK, Cherry KM, Kriger JN, Hrycan W, Marufu G, Thomas JD, Gray GR (2014) Photosynthetic responses to temperature-mediated dormancy induction in contrasting ecotypes of red-osier dogwood (Cornus sericea L.). Environ Exp Bot 106:221–230. doi: 10.1016/j.envexpbot.2014.02.015 CrossRefGoogle Scholar
- Tsormpatsidis E, Henbest RGC, Battey NH, Hadley P (2010) The influence of ultraviolet radiation on growth, photosynthesis and phenolic levels of green and red lettuce: potential for exploiting effects of ultraviolet radiation in a production system. Ann Appl Biol 156:357–366. doi: 10.1111/j.1744-7348.2010.00393.x CrossRefGoogle Scholar
- Yusuf MA, Kumar D, Rajwanshi R, Strasser RJ, Tsimilli-Michael M, Govindjee Sarin NB (2010) Overexpression of γ-tocopherol methyl transferase gene in transgenic Brassica juncea plants alleviates abiotic stress: physiological and chlorophyll a fluorescence measurements. Biochim Biophys Acta 1797:1428–1438. doi: 10.1016/j.bbabio.2010.02.002 CrossRefPubMedGoogle Scholar