Photochemistry of thylakoid membranes in two pea cultivars with different leaf colouration

  • Selma Mlinarić
  • Tihomir Čupić
  • Svetislav Popović
  • Vlatka Jurković
  • Lidija Begović
  • Vera Cesar
  • Hrvoje LepedušEmail author


Photochemical features of thylakoid membranes and chlorophyll content were investigated in two pea (Pisum sativum L.) cultivars with different leaf colouration: ‘Assas’ (red leaves) and ‘Arvika’ (green leaves). In vivo measured chlorophyll fluorescence (OJIP) was used to evaluate overall photosynthetic efficiency (expressed as performance index, PIABS) as well as for advanced analysis of main photochemical processes per active reaction centre (RC) of photosystem II (PSII). To evaluate the response of PSII driven linear electron transport (relETR) and non-photochemical quenching (NPQ), the plants were challenged with short-term high light (~2000 µmol m−2 s−1). In spite of prevailing red colour, leaves of ‘Assas’ had identical concentrations of chlorophylls a and b as green ones. The OJIP transients showed that red leaves grown in field (500–800 µmol m−2 s−1) had decreased PIABS and electron transport per RC beyond primary acceptor (Q A ) compared to green leaves. After high light exposure red leaves revealed impaired relETR accompanied with increased NPQ values. Anthocyanins located in epidermal cells affected neither chlorophylls concentrations nor the light capture features of PSII. Despite equal concentrations of chlorophylls and PSII photochemistry further than Q A in both leaf types, red leaves reduced overall photosynthetic efficiency due to impaired relETR in thylakoid membranes.


Pisum sativum L. Anthocyanins Chlorophyll fluorescence Photosynthesis Photoprotection 



Financial support for the work was provided by the Croatian Ministry of Science, Education and Sport (Grants 073-0731674-1673 to HL, 073-0000000-3535 to SP and 073-0731674-0841 to VC).


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Copyright information

© Brazilian Society of Plant Physiology 2017

Authors and Affiliations

  • Selma Mlinarić
    • 1
  • Tihomir Čupić
    • 2
  • Svetislav Popović
    • 2
  • Vlatka Jurković
    • 2
  • Lidija Begović
    • 1
  • Vera Cesar
    • 1
  • Hrvoje Lepeduš
    • 3
    Email author
  1. 1.Department of BiologyJosip Juraj Strossmayer University of OsijekOsijekCroatia
  2. 2.Agricultural Institute OsijekOsijekCroatia
  3. 3.Faculty of Humanities and Social SciencesJosip Juraj Strossmayer University of OsijekOsijekCroatia

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