High level of sucrose, spermine and spermidine are related with the early germination in Plathymenia foliolosa compared to Dalbergia nigra

de Souza Rios, Ana Paula; Aragão, Victor Paulo Mesquita; Passamani, Lucas Zanchetta; Macedo, Amanda Ferreira; Fulgêncio, Luís André Poiares; Floh, Eny Iochevet Segal; Barroso, Deborah Guerra; Silveira, Vanildo; Santa-Catarina, Claudete

doi:10.1007/s40626-015-0048-0

Published: 10 December 2015

High level of sucrose, spermine and spermidine are related with the early germination in Plathymenia foliolosa compared to Dalbergia nigra

Ana Paula de Souza Rios¹,
Victor Paulo Mesquita Aragão¹,
Lucas Zanchetta Passamani²,
Amanda Ferreira Macedo³,
Luís André Poiares Fulgêncio¹,
Eny Iochevet Segal Floh³,
Deborah Guerra Barroso⁴,
Vanildo Silveira² &
Claudete Santa-Catarina¹

Theoretical and Experimental Plant Physiology volume 27, pages 237–249 (2015)Cite this article

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Abstract

Seeds are the major organs responsible for the continuation of the plant’s life cycle. The aim was to investigate the relationship of soluble sugars, polyamines and amino acids on seed germination and early seedling growth in Plathymenia foliolosa Benth. and Dalbergia nigra (Vell.) Allemão ex Benth., two Fabaceae endangered species from Brazilian Atlantic Forest. Analyses were performed in mature seeds (before) and after 1, 2, 5 and 8 days of imbibition for P. foliolosa and 2, 5, 8 and 11 days for D. nigra, corresponding to imbibition (phase I), root protrusion (phase II), root elongation and seedling (phase III) phases. Seeds of P. foliolosa complete germination before of D. nigra. The higher content of sucrose in the mature dry seed, and increasing contents of free spermidine and spermine during imbibition (phase I) and radicle protrusion (phase II) in P. foliolosa seeds could be related with the early germination in this species. The higher glucose contents and presence of maltose in mature dry seed and at the phases I and II might be associated with a more time necessary for seed germination in D. nigra. Free putrescine and fructose contents are necessary to seedling growth (phase III) in both species. This work is the first to report an association between these compounds during seed germination and early seedling growth in these species. This characterization can contribute to biochemical network of seed germination in wood species, and can be used in conservation programs of endangered species, as well as in biotechnological approaches to improve alternative methods of in vitro propagation, as somatic embryogenesis.

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Abbreviations

ANOVA:: Analysis of variance
DM:: Dry matter
FM:: Fresh matter
GABA:: γ-Aminobutyric acid
HPLC:: High performance liquid chromatography
OPA:: o-phthaldialdehyde
PA:: Polyamine
Put:: Putrescine
Spd:: Spermidine
Spm:: Spermine
WC:: Water content

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Acknowledgments

This research was supported by the Carlos Chagas Filho Foundation for Research Support in the State of Rio de Janeiro—FAPERJ (E26/111.389-2012, E26/102.989/2012 and E26/010.001507/2014) and the National Council for Scientific and Technological Development—CNPq (305645/2013-7 and 444453/2014-8). Scholarships were granted by Coordination for the Improvement of Higher Education Personnel—CAPES to APSR, LZP and VPMA.

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Authors and Affiliations

Laboratório de Biologia Celular e Tecidual (LBCT)-CBB, Universidade Estadual do Norte Fluminense, Avenida Alberto Lamego, 2000, Parque Califórnia, Campos Dos Goytacazes, RJ, CEP: 28013-602, Brazil
Ana Paula de Souza Rios, Victor Paulo Mesquita Aragão, Luís André Poiares Fulgêncio & Claudete Santa-Catarina
Laboratório de Biotecnologia (LBT)-CBB, Universidade Estadual do Norte Fluminense, Campos Dos Goytacazes, RJ, Brazil
Lucas Zanchetta Passamani & Vanildo Silveira
Laboratório de Biologia Celular de Plantas, Departamento de Botânica, IB-Universidade de São Paulo, São Paulo, Brazil
Amanda Ferreira Macedo & Eny Iochevet Segal Floh
Laboratório de Fitotecnia (LFIT)-CCTA, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos Dos Goytacazes, RJ, Brazil
Deborah Guerra Barroso

Authors

Ana Paula de Souza Rios
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Victor Paulo Mesquita Aragão
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Lucas Zanchetta Passamani
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Amanda Ferreira Macedo
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Luís André Poiares Fulgêncio
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Eny Iochevet Segal Floh
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Deborah Guerra Barroso
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Vanildo Silveira
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Claudete Santa-Catarina
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Correspondence to Claudete Santa-Catarina.

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de Souza Rios, A.P., Aragão, V.P.M., Passamani, L.Z. et al. High level of sucrose, spermine and spermidine are related with the early germination in Plathymenia foliolosa compared to Dalbergia nigra . Theor. Exp. Plant Physiol. 27, 237–249 (2015). https://doi.org/10.1007/s40626-015-0048-0

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Received: 24 November 2015
Accepted: 26 November 2015
Published: 10 December 2015
Issue Date: December 2015
DOI: https://doi.org/10.1007/s40626-015-0048-0

Keywords

Amino acids
Biochemical analyses
Fabaceae
HPLC analyses
Polyamines
Threatened woody species