Atypical hemolytic uremic syndrome (aHUS) is a rare disease in which uncontrolled terminal complement activation leads to systemic thrombotic microangiopathy (TMA). Pregnancy can trigger aHUS and, without complement inhibition, many women with pregnancy-triggered aHUS (p-aHUS) progress to end-stage renal disease (ESRD) with a high risk of morbidity. Owing to relatively small patient numbers, published characterizations of p-aHUS have been limited, thus the Global aHUS Registry (NCT01522183, April 2012) provides a unique opportunity to analyze data from a large single cohort of women with p-aHUS.
The demographics and clinical characteristics of women with p-aHUS (n = 51) were compared with those of women of childbearing age with aHUS and no identified trigger (non-p-aHUS, n = 397). Outcome evaluations, including renal survival according to time to ESRD, were compared for patients with and without eculizumab treatment (a complement C5 inhibitor) in both aHUS groups.
Baseline demographics and clinical characteristics were broadly similar in both groups. The proportion of women with p-aHUS and non-p-aHUS with pathogenic variant(s) in complement genes and/or anti-complement factor H antibodies was similar (45% and 43%, respectively), as was the proportion with a family history of aHUS (12% and 13%, respectively). Eculizumab treatment led to significantly improved renal outcomes in women with aHUS, regardless of whether aHUS was triggered by pregnancy or not: adjusted hazard ratio for time to ESRD was 0.06 (p = 0.006) in the p-aHUS group and 0.20 (p < 0.0001) in the non-p-aHUS group.
Findings from this study support the characterization of p-aHUS as a complement-mediated TMA.
Atypical hemolytic uremic syndrome (aHUS) is a rare disease caused by dysregulation of the alternative pathway of complement. The resulting uncontrolled terminal complement activation causes inflammation, endothelial activation and damage, and a pro-thrombotic/pro-anticoagulant state leading to systemic thrombotic microangiopathy (TMA) [1,2,3]. Patients with aHUS are at risk of unpredictable and/or progressive TMA-mediated damage to renal and other organ systems, leading to severe morbidity and premature death [4,5,6]. Identified triggers of aHUS include pregnancy, infection, autoimmune conditions, organ transplants, and certain drug treatments [7,8,9].
Pregnancy-triggered aHUS (p-aHUS) presents during pregnancy or postpartum and has been estimated to account for approximately 7% of all cases with aHUS and up to 20% of cases in women [5, 10]. In a 2019 retrospective study of a French cohort with adjudicated TMAs, Bayer et al. reported that among patients with identified causes of TMA, pregnancy was the leading cause of secondary TMA (35%) . p-aHUS is associated with high perinatal or maternal morbidity and mortality, with many women progressing to end-stage renal disease (ESRD) [7, 10, 11].
Without targeted treatment, outcomes of patients with aHUS are poor. Despite the use of plasma exchange (PE) or plasma infusion (PI), more than half of patients progress to ESRD or death [4,5,6]. The terminal complement C5 inhibitors, eculizumab and ravulizumab, are targeted treatments approved for patients with aHUS [12,13,14,15]. The efficacy and safety of eculizumab (first approved in 2011) in the treatment of aHUS has been demonstrated in four prospective clinical trials and has been supported by additional data from registries and other real-world patient studies [16,17,18,19,20,21,22].
Given the rarity of aHUS, the Global aHUS Registry provides a unique opportunity to characterize the disease further, using data from multiple participating centers worldwide. The objective of this study was to use Global aHUS Registry data to compare the clinical characteristics and renal outcomes, with and without eculizumab treatment, in women with p-aHUS with those in women of childbearing age with aHUS but without identified triggers.
Patients with a clinical diagnosis of aHUS were included in the observational non-interventional Global aHUS Registry (NCT01522183) . This registry was initiated in April 2012 to evaluate the clinical outcomes of patients with aHUS irrespective of the treatment modality used . The registry study was established in accordance with the International Conference on Harmonisation Good Clinical Practice Guidelines and the Declaration of Helsinki. All patients provided written Informed Consent before participation.
Patients meeting the registry inclusion criteria were males or females of any age with a diagnosis of aHUS, with or without an identified complement pathogenic variant or anti-complement factor H (CFH) antibody . Patients with evidence of Shiga toxin-producing Escherichia coli infection and those with a disintegrin and metalloproteinase with a thrombospondin type 1 motif-13 (ADAMTS13) activity level of 5% or higher (the level consistent with a diagnosis of thrombotic thrombocytopenia purpura), if performed, were excluded .
In this analysis, patients with p-aHUS were identified in the Global aHUS Registry as female patients with first TMA manifestations/complications during pregnancy or within 60 days postpartum. Only women with at least 90 days of follow-up after initial TMA manifestations/complications were included. Women were excluded if they had any other identified trigger of aHUS (history of drug-induced aHUS; first onset of symptoms within 14 days of Streptococcus pneumoniae infection; first onset of symptoms within 1 year of a bone marrow transplant; or coexisting autoimmune conditions identified by the treating physician [no further details recorded], at the time of initial TMA complications/manifestation) or if they discontinued the registry or eculizumab treatment owing to an alternative diagnosis. A comparator group of women with aHUS not triggered by pregnancy (non-p-aHUS), was comprised of female patients in the Global aHUS Registry of childbearing age (18–51 years), with at least 90 days of follow-up after initial TMA manifestations/complications and no other identified trigger of aHUS or alternative diagnosis (as described in the exclusion criteria above).
Descriptive statistics were used to identify similarities and differences between the p-aHUS and non-p-aHUS groups in terms of baseline demographics and clinical characteristics, including treatments received. Women in each group were stratified into those treated with eculizumab (at least one dose; 600, 900 or 1200 mg) and those not treated with eculizumab, the latter including those never treated with eculizumab as well as those who did not receive eculizumab prior to ESRD. Cox regression was used to compare renal prognoses between aHUS groups and between women treated and not treated with eculizumab. The hazard ratio (HR) based on time to ESRD after initial TMA manifestation was calculated to compare outcomes for patients with and without eculizumab treatment. The unadjusted HR was calculated as the risk of ESRD in women treated with eculizumab divided by the risk of ESRD in those not treated with eculizumab. In addition, HRs adjusted for the following covariates were calculated: (1) dialysis and/or PE/PI treatment, and (2) at least one complement gene mutation and/or anti-CFH antibody positive status, at the time of initial TMA.
As of January 13, 2020, 1858 patients were enrolled in the Global aHUS Registry, including 1029 female patients. For this study, 51 and 397 women of childbearing age were identified with p-aHUS or non-p-aHUS, respectively, after specific inclusion and exclusion criteria were applied. The groups used for comparative analyses are shown in Fig. 1.
Baseline demographics and clinical characteristics
Age at aHUS diagnosis was similar for women with p-aHUS and non-p-aHUS (mean ± standard deviation [SD] 31.2 ± 5.9 years and 29.1 ± 11.0 years, respectively) and across treatment subgroups (Table 1). The mean time from initial TMA manifestation to aHUS diagnosis was shorter in the p-aHUS group compared with the non-p-aHUS group (mean ± SD 0.5 ± 1.4 months and 4.5 ± 40.6 months, respectively). The proportion of women with a family history of aHUS was similar across treatment subgroups, ranging from approximately 11.1% to 14.6% (Table 1). In 50/51 (98.0%) patients with p-aHUS, the index pregnancy was their first pregnancy, and no pregnancies were reported after initial TMA complications. In women with non-p-aHUS, 33/395 (8.4%) had pregnancies prior to enrollment or while enrolled, of which approximately half (18/33) were prior to initial TMA complications. Just over half of women with p-aHUS experienced initial TMA manifestations/complications during pregnancy (54.9% during pregnancy and 45.1% postpartum). The trimester in which initial TMA complications occurred was only recorded for 28/51 women (Table 1).
Overall, 12/51 (23.5%) women with p-aHUS had a kidney transplant(s), all after their index pregnancy. Of the women with non-p-aHUS, 136/395 (34.4%) underwent a kidney transplant: 34 (8.6%) prior to aHUS diagnosis and 113 (28.6%) after diagnosis (not mutually exclusive). Kidney transplantations after index pregnancy or aHUS diagnosis were more common in women who did not receive eculizumab treatment compared with those who were treated with eculizumab (10/24 [41.7%] vs 2/27 [7.4%] in women with p-aHUS and 98/198 [49.5%] vs 15/187 [8.0%] in those with non-p-aHUS, respectively) (Table 1).
The proportion of women undergoing dialysis at the time of initial TMA manifestation was comparable across all groups (Table 1). The proportion of women with extra-renal manifestations (cardiovascular, pulmonary, central nervous system-related, or gastrointestinal) at the time of initial TMA was similar for women with p-aHUS and non-p-aHUS, and in both groups this baseline proportion was higher in eculizumab-treated women compared with those not treated with eculizumab (Table 1).
Twenty-seven (52.9%) women with p-aHUS and 187 (47.3%) women with non-p-aHUS received eculizumab treatment. The mean ± SD duration of eculizumab treatment was 1.78 ± 1.76 years and 2.87 ± 2.35 years, and the mean ± SD time from initial TMA to treatment initiation was 0.07 ± 0.13 years and 1.64 ± 4.78 years in women with p-aHUS and non-p-aHUS, respectively.
Overall, the proportion of women who had undergone PE/PI at any time was similar for those with p-aHUS and non-p-aHUS (78.4% and 70.9%, respectively, Table 1); for those treated with eculizumab, 22/27 (81.5%) women with p-aHUS and 125/187 (66.8%) women with non-p-aHUS had undergone PE/PI prior to eculizumab. The duration of PE/PI treatment was longer for women with non-p-aHUS (mean ± SD duration 16.7 ± 19.9 days and 129.8 ± 531.8 days for those with p-aHUS and non-p-aHUS, respectively). The duration of PE/PI was shorter for women treated with eculizumab compared with those not treated with eculizumab in both aHUS groups (13.2 ± 16.1 days vs 20.9 ± 23.4 days in those with p-aHUS and 41.2 ± 154.5 days vs 211.8 ± 713.1 days in those with non-p-aHUS). These findings were irrespective of whether PE/PI treatment was undergone prior to or after eculizumab treatment.
The prevalence of pathogenic variants in complement genes and anti-CFH antibodies was compared for both aHUS groups (Table 2). The complement genes tested were: complement C3; complement factors H, I, and B; complement CD46 (membrane cofactor protein); and thrombomodulin. The complement genetics and antibody status of women with p-aHUS and non-p-aHUS were similar; the proportion of those with pathogenic variant(s) in complement genes and/or anti-CFH antibody was 45.1% (23/51) and 42.8% (170/397), respectively.
The mean ± SD follow-up period was 3.73 ± 2.01 years and 4.00 ± 1.97 years for women with p-aHUS and non-p-aHUS, respectively. At the last follow-up, the numbers of women with p-aHUS and non-p-aHUS receiving eculizumab treatment were 18 (35.3%) and 179 (45.1%), respectively.
Renal outcomes were evaluated by measuring the time from initial TMA manifestation to ESRD and the change in estimated glomerular filtration rate (eGFR). Kaplan–Meier curves were generated to compare the time from initial TMA manifestation to ESRD with and without eculizumab treatment (Fig. 2). The risk of ESRD was significantly higher for women not treated with eculizumab, compared with eculizumab-treated women, in both aHUS groups: the unadjusted HR was 0.14 (95% confidence interval [CI] 0.04, 0.47; p = 0.002) in the p-aHUS group and 0.16 (95% CI 0.11, 0.24; p < 0.0001) in the non-p-aHUS group. The HR adjusted for dialysis, PE/PI treatments, and at least one complement gene mutation and/or anti-CFH antibody positive status, at the time of initial TMA was 0.08 (95% CI 0.01, 0.65; p = 0.019) and 0.19 (95% CI 0.10, 0.36; p < 0.0001), respectively. In both aHUS groups, the eGFR improved after eculizumab treatment, with a mean ± SD increase relative to baseline of 56.2 ± 39.8 and 40.9 ± 32.1 mL/min/1.73 m2, for patients with p-aHUS and non-p-aHUS, respectively.
The proportion of women with reported new onset cardiovascular, central nervous system, or gastrointestinal manifestations was comparable between the aHUS groups and ranged from 17.1% to 25.9% across all treatment groups. In women with p-aHUS, pulmonary manifestations were reported for 25.9% and 0% of those treated and not treated with eculizumab, respectively, compared with approximately 8% of women with non-p-aHUS (both treated and not treated with eculizumab).
Pregnancy complications were reported for women with p-aHUS. Overall, 54.9% had pre-eclampsia and 33.3% HELLP syndrome, with no notable differences between women treated with eculizumab and those not treated with eculizumab. Cesarean sections were performed for 29.6% and 20.8% of women treated and not treated with eculizumab, respectively.
Previous studies of p-aHUS have helped to characterize this subset of the aHUS population; however, direct comparisons with aHUS not associated with identifiable triggers have not been feasible . The current analysis compared characteristics and renal outcomes in women with p-aHUS to those of childbearing age with aHUS but no associated triggers (non-p-aHUS), based on data retrieved from the Global aHUS Registry as a source of a single, large cohort. There were no eligible women with p-aHUS younger than 18 years in the Global aHUS Registry, therefore, for the purposes of this study, the inclusion criteria for the comparator group of ‘women of childbearing age’ specified a minimum age of 18 years. Our results showed that both groups were similar in several demographic and clinical characteristics, as well as in their response to eculizumab.
In this study, 43% of women in the p-aHUS group had pathogenic variant(s) in complement genes or anti-CFH antibodies and the proportion was similar in the non-p-aHUS comparator group (45%). These percentages are within the range of reported rates of complement genetic abnormalities in patients with aHUS (45–70%) [5, 6, 26, 27]. The proportion of women with a family history of aHUS was also similar in both groups.
The proportion of women on dialysis at the time of initial TMA manifestations was 14% and 11% for the p-aHUS and non-p-aHUS groups, respectively, indicating a similarity in renal function. Furthermore, the rate of kidney transplants for women treated versus not treated with eculizumab was similar within both aHUS groups (higher for those not treated with eculizumab), indicating a similarity in renal prognosis in women with p-aHUS and women with aHUS not triggered by pregnancy.
Extra-renal manifestations are common in patients with aHUS and have been hypothesized to be related to acute and chronic complement activation and dysregulation . In an assessment of data from the Global aHUS Registry by Schaefer et al., extra-renal manifestations were reported for 19–38% of patients within the initial presenting phase, prior to eculizumab treatment, with gastrointestinal manifestations being the most prevalent . The similar frequencies of extra-renal manifestations in the aHUS groups in this study again suggest the same disease presentation. Extra-renal manifestations at baseline were more prevalent in women later treated with eculizumab compared with those not treated with eculizumab in both aHUS groups. It is possible that physicians consider these symptoms a risk factor for TMA and hence these women are more likely to be diagnosed and treated; this may explain why a higher prevalence of extra-renal manifestations in women treated with eculizumab was found in this study.
Treatment with eculizumab significantly reduced the risk of ESRD in women with p-aHUS and non-p-aHUS, compared with no eculizumab treatment. It is acknowledged that, owing to the non-interventional nature of this study, comparison of outcomes for women treated and not treated with eculizumab may be subject to bias, as treatment decisions by physicians were not protocolized and could be influenced by a number of presenting factors. Cox regression modeling of the data for treated versus not treated groups was therefore adjusted for covariates deemed likely to influence treatment decisions (namely dialysis and/or PE/PI treatment at the time of initial TMA, and complement gene mutations and/or anti-CFH antibodies). A reduced risk of ESRD in women treated with eculizumab, compared with those not treated with eculizumab, was observed even when the data were adjusted for these covariates (for both aHUS groups). Improved renal outcomes for women in both aHUS groups treated with eculizumab were also indicated by an increase in mean eGFR from baseline. Similarity in response of complement C5 inhibition indicates similar disease pathophysiology in p-aHUS and aHUS not associated with identifiable triggers.
In women with p-aHUS, 98% of TMAs were reported during first pregnancy. This is a greater proportion than has been reported in the literature (40–70%) ; however, reporting bias of pregnancy history cannot be ruled out because previous pregnancies with no complications may be under-reported. Just over half of initial TMA manifestations/complications occurred during pregnancy, with the rest occurring within 60 days postpartum. This is in contrast to reports in the literature in which p-aHUS incidence is reported to be higher postpartum [10, 11, 29]. Diagnosis of p-aHUS is often delayed owing to the overlapping clinical and laboratory features of p-aHUS with other known pregnancy complications such as pre-eclampsia and HELLP syndrome; therefore, the proportion of cases occurring during pregnancy may be under-estimated in the literature [30, 31]. In the current study, a high rate of pre-eclampsia (55%) and HELLP syndrome (33%) was also reported, with many women reporting both (20%).
Approximately half of the women in this study were not treated with eculizumab, many of whom had received a diagnosis prior to the availability of this complement C5 inhibitor in 2011 (during the time period 1985–2010, initial TMA manifestations were reported for 19.6% and 36.2% of women in the p-a-HUS and non-p-aHUS groups, respectively). The mean time from initial TMA manifestation to aHUS diagnosis was longer in women with non-p-aHUS than in those with p-aHUS, and in women not treated with eculizumab than in those treated with eculizumab. This may in part be explained by the higher proportion of women in the non-p-aHUS and non-eculizumab-treated groups who experienced initial TMA manifestations during an earlier timeframe, when aHUS was less widely recognized. The earlier date of initial TMA manifestations may also explain the longer mean time from initial TMA manifestation to treatment initiation in women with non-p-aHUS. Pregnancy has been increasingly recognized as a trigger for aHUS involving complement over-activation, which may be the reason for the shorter length of time from initial TMA to aHUS diagnosis and initiation of eculizumab treatment in the p-aHUS group, compared with the non-p-aHUS group [11, 29]. The duration of PE/PI treatment was shorter in the p-aHUS group than in the non-p-aHUS group and it was shorter for women treated with eculizumab than those not treated with eculizumab, likely owing to the discontinuation of PE/PI upon the initiation of eculizumab as standard of care treatment.
The findings from this study support the classification of p-aHUS as a complement-mediated TMA, based on clinical characteristics, complement genetics, and response to eculizumab treatment. It is acknowledged that there are some limitations to this study, owing to its observational design and the real-world setting of the Global aHUS Registry, including missing data for some women, the fact that not all women treated with eculizumab were on the same dosing regimen (and some had an unreported dose level), and potential variation in interpretation of disease characteristics.
Findings from this large collection of data from a single, real-world, global cohort confirm that pregnancy-triggered aHUS is comparable to aHUS with no identified trigger. Our results indicate that pregnancy-triggered aHUS is not only a disease of the postpartum period, and that presumed pregnancy-associated TMAs may well be aHUS. The similarities in demographics, clinical characteristics, complement genetics, disease progression, and response to eculizumab between women in both groups confirm that pregnancy-triggered aHUS can be considered a complement-mediated TMA.
Availability of data and materials
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George JN, Nester CM (2014) Syndromes of thrombotic microangiopathy. NEJM 371(7):654–666. https://doi.org/10.1056/NEJMra1312353
Campistol JM, Arias M, Ariceta G, Blasco M, Espinosa L, Espinosa M, Grinyó JM, Macía M, Mendizábal S, Praga M, Román E, Torra R, Valdés F, Vilalta R, Rodríguez de Córdoba S (2015) An update for atypical haemolytic uraemic syndrome: diagnosis and treatment. A consensus document. Nefrologia 35(5):421–447. https://doi.org/10.1016/j.nefro.2015.07.005
Fakhouri F, Zuber J, Frémeaux-Bacchi V, Loirat C (2017) Haemolytic uraemic syndrome. Lancet 390(10095):681–696. https://doi.org/10.1016/S0140-6736(17)30062-4
Sellier-Leclerc AL, Fremeaux-Bacchi V, Dragon-Durey MA, Macher MA, Niaudet P, Guest G, Boudailliez B, Bouissou F, Deschenes G, Gie S, Tsimaratos M, Fischbach M, Morin D, Nivet H, Alberti C, Loirat C (2007) Differential impact of complement mutations on clinical characteristics in atypical hemolytic uremic syndrome. J Am Soc Nephrol 18(8):2392–2400. https://doi.org/10.1681/asn.2006080811
Fremeaux-Bacchi V, Fakhouri F, Garnier A, Bienaimé F, Dragon-Durey MA, Ngo S, Moulin B, Servais A, Provot F, Rostaing L, Burtey S, Niaudet P, Deschênes G, Lebranchu Y, Zuber J, Loirat C (2013) Genetics and outcome of atypical hemolytic uremic syndrome: a nationwide French series comparing children and adults. Clin J Am Soc Nephrol 8(4):554–562. https://doi.org/10.2215/cjn.04760512
Noris M, Caprioli J, Bresin E, Mossali C, Pianetti G, Gamba S, Daina E, Fenili C, Castelletti F, Sorosina A, Piras R, Donadelli R, Maranta R, van der Meer I, Conway EM, Zipfel PF, Goodship TH, Remuzzi G (2010) Relative role of genetic complement abnormalities in sporadic and familial aHUS and their impact on clinical phenotype. Clin J Am Soc Nephrol 5(10):1844–1859. https://doi.org/10.2215/cjn.02210310
Nester CM, Barbour T, de Cordoba SR, Dragon-Durey MA, Fremeaux-Bacchi V, Goodship TH, Kavanagh D, Noris M, Pickering M, Sanchez-Corral P, Skerka C, Zipfel P, Smith RJ (2015) Atypical aHUS: state of the art. Mol Immunol 67(1):31–42. https://doi.org/10.1016/j.molimm.2015.03.246
Goodship TH, Cook HT, Fakhouri F, Fervenza FC, Frémeaux-Bacchi V, Kavanagh D, Nester CM, Noris M, Pickering MC, Rodríguez de Córdoba S, Roumenina LT, Sethi S, Smith RJ (2017) Atypical hemolytic uremic syndrome and C3 glomerulopathy: conclusions from a “Kidney Disease: Improving Global Outcomes” (KDIGO) Controversies Conference. Kidney Int 91(3):539–551. https://doi.org/10.1016/j.kint.2016.10.005
Bayer G, von Tokarski F, Thoreau B, Bauvois A, Barbet C, Cloarec S, Mérieau E, Lachot S, Garot D, Bernard L, Gyan E, Perrotin F, Pouplard C, Maillot F, Gatault P, Sautenet B, Rusch E, Buchler M, Vigneau C, Fakhouri F, Halimi J-M (2019) Etiology and outcomes of thrombotic microangiopathies. Clin J Am Soc Nephrol 14(4):557. https://doi.org/10.2215/CJN.11470918
Fakhouri F, Roumenina L, Provot F, Sallée M, Caillard S, Couzi L, Essig M, Ribes D, Dragon-Durey M-A, Bridoux F, Rondeau E, Frémeaux-Bacchi V (2010) Pregnancy-associated hemolytic uremic syndrome revisited in the era of complement gene mutations. J Am Soc Nephrol JASN 21(5):859–867. https://doi.org/10.1681/ASN.2009070706
Bruel A, Kavanagh D, Noris M, Delmas Y, Wong EKS, Bresin E, Provôt F, Brocklebank V, Mele C, Remuzzi G, Loirat C, Frémeaux-Bacchi V, Fakhouri F (2017) Hemolytic uremic syndrome in pregnancy and postpartum. Clin J Am Soc Nephrol 12(8):1237–1247. https://doi.org/10.2215/cjn.00280117
European Medicines Agency Ultomiris (ravulizumab). https://www.ema.europa.eu/en/medicines/human/EPAR/ultomiris. Accessed Nov 2020
European Medicines Agency Soliris (eculizumab). https://www.ema.europa.eu/en/documents/product-information/soliris-epar-product-information_en.pdf. Accessed Nov 2020
US Food and Drug Administration Soliris (eculizumab). https://www.accessdata.fda.gov/drugsatfda_docs/label/2019/125166s431lbl.pdf. Accessed Nov 2020
US Food and Drug Administration Ultomiris (ravuliazumab-cwvz). https://www.accessdata.fda.gov/drugsatfda_docs/label/2019/761108s001lbl.pdf. Accessed Nov 2020
Legendre CM, Licht C, Muus P, Greenbaum LA, Babu S, Bedrosian C, Bingham C, Cohen DJ, Delmas Y, Douglas K, Eitner F, Feldkamp T, Fouque D, Furman RR, Gaber O, Herthelius M, Hourmant M, Karpman D, Lebranchu Y, Mariat C, Menne J, Moulin B, Nurnberger J, Ogawa M, Remuzzi G, Richard T, Sberro-Soussan R, Severino B, Sheerin NS, Trivelli A, Zimmerhackl LB, Goodship T, Loirat C (2013) Terminal complement inhibitor eculizumab in atypical hemolytic-uremic syndrome. N Engl J Med 368(23):2169–2181. https://doi.org/10.1056/NEJMoa1208981
Rondeau E, Cataland SR, Al-Dakkak I, Miller B, Webb NJA, Landau D (2019) Eculizumab safety: five-year experience from the global atypical hemolytic uremic syndrome registry. Kidney Int Rep 4(11):1568–1576. https://doi.org/10.1016/j.ekir.2019.07.016
Siedlecki AM, Isbel N, Vande Walle J, James Eggleston J, Cohen DJ, Global aHUS Registry (2018) Eculizumab use for kidney transplantation in patients with a diagnosis of atypical hemolytic uremic syndrome. Kidney Int Rep 4(3):434–446. https://doi.org/10.1016/j.ekir.2018.11.010
Zuber J, Frimat M, Caillard S, Kamar N, Gatault P, Petitprez F, Couzi L, Jourde-Chiche N, Chatelet V, Gaisne R, Bertrand D, Bamoulid J, Louis M, Sberro Soussan R, Navarro D, Westeel PF, Frimat L, Colosio C, Thierry A, Rivalan J, Albano L, Arzouk N, Cornec-Le Gall E, Claisse G, Elias M, El Karoui K, Chauvet S, Coindre JP, Rerolle JP, Tricot L, Sayegh J, Garrouste C, Charasse C, Delmas Y, Massy Z, Hourmant M, Servais A, Loirat C, Fakhouri F, Pouteil-Noble C, Peraldi MN, Legendre C, Rondeau E, Le Quintrec M, Frémeaux-Bacchi V (2019) Use of highly individualized complement blockade has revolutionized clinical outcomes after kidney transplantation and renal epidemiology of atypical hemolytic uremic syndrome. J Am Soc Nephrol 30(12):2449–2463. https://doi.org/10.1681/asn.2019040331
Licht C, Greenbaum LA, Muus P, Babu S, Bedrosian CL, Cohen DJ, Delmas Y, Douglas K, Furman RR, Gaber OA, Goodship T, Herthelius M, Hourmant M, Legendre CM, Remuzzi G, Sheerin N, Trivelli A, Loirat C (2015) Efficacy and safety of eculizumab in atypical hemolytic uremic syndrome from 2-year extensions of phase 2 studies. Kidney Int 87(5):1061–1073. https://doi.org/10.1038/ki.2014.423
Greenbaum LA, Fila M, Ardissino G, Al-Akash SI, Evans J, Henning P, Lieberman KV, Maringhini S, Pape L, Rees L, van de Kar NCAJ, Vande Walle J, Ogawa M, Bedrosian CL, Licht C (2016) Eculizumab is a safe and effective treatment in pediatric patients with atypical hemolytic uremic syndrome. Kidney Int 89(3):701–711. https://doi.org/10.1016/j.kint.2015.11.026
Fakhouri F, Hourmant M, Campistol JM, Cataland SR, Espinosa M, Gaber AO, Menne J, Minetti EE, Provot F, Rondeau E, Ruggenenti P, Weekers LE, Ogawa M, Bedrosian CL, Legendre CM (2016) Terminal complement inhibitor eculizumab in adult patients with atypical hemolytic uremic syndrome: a single-arm, open-label trial. Am J Kidney Dis 68(1):84–93. https://doi.org/10.1053/j.ajkd.2015.12.034
CinicalTrials.gov (2012) Atypical hemoloytic urermic syndrome (aHUS) registry (NCT01522183)
Licht C, Ardissino G, Ariceta G, Cohen D, Cole JA, Gasteyger C, Greenbaum LA, Johnson S, Ogawa M, Schaefer F, Vande Walle J, Frémeaux-Bacchi V (2015) The global aHUS registry: methodology and initial patient characteristics. BMC Nephrol 16:207–207. https://doi.org/10.1186/s12882-015-0195-1
Gupta M, Govindappagari S, Burwick RM (2020) Pregnancy-associated atypical hemolytic uremic syndrome: a systematic review. Obstet Gynecol 135(1):46–58
Schaefer F, Ardissino G, Ariceta G, Fakhouri F, Scully M, Isbel N, Lommele A, Kupelian V, Gasteyger C, Greenbaum LA, Johnson S, Ogawa M, Licht C, Vande Walle J, Fremeaux-Bacchi V (2018) Clinical and genetic predictors of atypical hemolytic uremic syndrome phenotype and outcome. Kidney Int 94(2):408–418. https://doi.org/10.1016/j.kint.2018.02.029
Bu F, Zhang Y, Wang K, Borsa NG, Jones MB, Taylor AO, Takanami E, Meyer NC, Frees K, Thomas CP, Nester C, Smith RJH (2018) Genetic analysis of 400 patients refines understanding and implicates a new gene in atypical hemolytic uremic syndrome. J Am Soc Nephrol 29(12):2809–2819. https://doi.org/10.1681/asn.2018070759
Hofer J, Rosales A, Fischer C, Giner T (2014) Extra-renal manifestations of complement-mediated thrombotic microangiopathies. Front Pediatr. https://doi.org/10.3389/fped.2014.00097
Huerta A, Arjona E, Portoles J, Lopez-Sanchez P, Rabasco C, Espinosa M, Cavero T, Blasco M, Cao M, Manrique J, Cabello-Chavez V, Suñer M, Heras M, Fulladosa X, Belmar L, Sempere A, Peralta C, Castillo L, Arnau A, Praga M, Rodriguez de Cordoba S (2018) A retrospective study of pregnancy-associated atypical hemolytic uremic syndrome. Kidney Int 93(2):450–459. https://doi.org/10.1016/j.kint.2017.06.022
Shanmugalingam R, Hsu D, Makris A (2018) Pregnancy-induced atypical haemolytic uremic syndrome: a new era with eculizumab. Obstet Med 11(1):28–31. https://doi.org/10.1177/1753495x17704563
Gupta M, Feinberg BB, Burwick RM (2018) Thrombotic microangiopathies of pregnancy: Differential diagnosis. Pregnancy Hypertens 12:29–34. https://doi.org/10.1016/j.preghy.2018.02.007
The sponsor and investigators thank the patients and their families for their participation in, and support for, this clinical study. The authors would also like to thank all the Global aHUS Registry investigators who have contributed data, Scientific Advisory Board members of the Global aHUS Registry: Christoph Licht, Véronique Frémeaux-Bacchi, Gema Ariceta, Larry Greenbaum, Sally Johnson, Franz Schaefer, Jeff Schmidt, Margriet Eygenraam and Christoph Gasteyger; and National Coordinators of the Global aHUS Registry: Miquel Blasco (Spain), Donata Cresseri (Italy), Galina Generolova (Russia), Nicholas Webb (United Kingdom), Patricia Hirt-Minkowski (Switzerland), Natalya Lvovna Kozlovskaya (Russia), Danny Landau (Israel), Anne-Laure Lapeyraque (Canada), Chantal Loirat (France), Christoph Mache (Austria), Michal Malina (Czech Republic), Leena Martola (Finland), Annick Massart (Belgium), Eric Rondeau (France), and Lisa Sartz (Sweden). The authors would like to acknowledge Sally McTaggart, PhD, Oxford Pharmagenesis for providing editorial and medical writing support with funding from Alexion Pharmaceuticals, Inc. and Radha Narayan, PhD, Alexion Pharmaceuticals, Inc. for critical review of the manuscript.
Open Access funding provided by Alexion Pharmaceuticals, Inc., Boston, MA. This analysis was funded by Alexion Pharmaceuticals, Inc. Alexion Pharmaceuticals, Inc. was responsible for the collection, management, and analysis of information contained in the Global aHUS Registry. Alexion Pharmaceuticals, Inc. contributed to data interpretation, preparation, review, and approval of the manuscript for submission. All authors had full access to all the data in the study and had final responsibility for the decision to submit for publication.
Conflict of interest
F Fakhouri has received fees for expertise, consultancy, and scientific symposia from Alexion Pharmaceuticals, Inc. M Scully has received speaker fees and is on an advisory board for Alexion Pharmaceuticals, Inc. G Ardissino has received speaker fees and is on an advisory board for Alexion Pharmaceuticals, Inc. I Al-Dakkak is an employee of, and may own stocks/options in Alexion Pharmaceuticals, Inc. B Miller was an employee of Alexion Pharmaceuticals, Inc. at the time of this study. E Rondeau has received fees for expertise, consultancy, and scientific symposia from Alexion Pharmaceuticals, Inc.
Consent to participate
A written informed consent was obtained from each patient prior to participation in the study. The Sponsor or its designee could provide an informed consent template to the sites, if required. If the site made any institution-specific modifications, the Sponsor or its designee could review the consent prior to IRB/EC submission. The Investigator or the Sponsor would then submit the approved, revised consent to the appropriate IRB/EC for review and approval prior to the start of the study. If the consent form was revised during the course of the study, all active participating patients to whom the revision may have had an impact must have signed the revised form. Before recruitment and enrollment, each patient was given a full explanation of the study and was allowed time to read the approved informed consent form. Once the Investigator was assured that the individual understood the implications of participating in the study, the patient was asked to give consent to participate in the study by signing the informed consent form. The Investigator provided a copy of the signed informed consent to the patient. The original form was maintained in the study files at the site.
Consent for publication
The sponsors of this study (Alexion Pharmaceuticals, Inc.) have provided consent for publication.
This was a multicenter study comprising many different sites of enrollment. Federal, provincial, and local regulations and International Conference on Harmonization (ICH) guidelines, if relevant, required that approval was obtained from an Ethics Committee (EC)/Independent review Board (IRB) prior to participation of patients in research studies. Where required and prior to the study onset, the EC/IRB must have approved the protocol, Informed Consent, advertisements to be used for patient recruitment, and any other written information regarding this study to be provided to the patient or the patient’s parents/legal guardian. The sites maintained and made available for review by the Sponsor or its designee documentation of all EC/IRB approvals and of the EC/IRB compliance with ICH Guidance E6: Good Clinical Practice, if relevant. All EC/IRB approvals were signed by the EC/IRB Chairman or designee and identified the EC/IRB name and address, the clinical protocol by title and/or protocol number, and the date approval and/or favorable opinion was granted. The Investigator conducted all aspects of this study in accordance with all national, provincial, and local laws of the pertinent regulatory authorities.
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Fakhouri, F., Scully, M., Ardissino, G. et al. Pregnancy-triggered atypical hemolytic uremic syndrome (aHUS): a Global aHUS Registry analysis. J Nephrol (2021). https://doi.org/10.1007/s40620-021-01025-x
- Atypical hemolytic uremic syndrome (aHUS)
- Complement-mediated TMA
- Complement C5 inhibitor
- End-stage renal disease (ESRD)