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Positive association between circulating Caveolin-1 and microalbuminuria in overt diabetes mellitus in pregnancy

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Abstract

Aims

Mounting evidence has shown that caveolin-1 plays a pathological role in the progression of albuminuria. Our study aimed to provide clinical evidence showing whether circulating caveolin-1 levels were associated with microalbuminuria (MAU) in women with overt diabetes mellitus in pregnancy (ODMIP).

Methods

A total of 150 pregnant women were enrolled in different groups, including 40 women with ODMIP and MAU (ODMIP + MAU), 40 women with ODMIP, and 70 women without ODMIP (Non-ODMIP). Plasma caveolin-1 levels were determined by ELISA. The presence of caveolin-1 in the human umbilical vein vascular wall was evaluated by immunohistochemical and western blot analysis, respectively. Albumin transcytosis across endothelial cells was measured using an established nonradioactive in vitro approach.

Results

Significantly increased levels of plasma caveolin-1 were detected in ODMIP + MAU women. The Pearson’s correlation analysis revealed a positive correlation between plasma caveolin-1 levels and Hemoglobin A1c (HbA1c %) as well as with MAU in the ODMIP + MAU group. Simultaneously, experimental knockdown or overexpression of caveolin-1 significantly decreased or increased the level of albumin transcytosis across both human and mouse glomerular endothelial cells (GECs), respectively.

Conclusions

Our data showed a positive association between plasma caveolin-1 levels and microalbuminuria in ODMIP + MAU.

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Availability of data and materials

Some or all datasets generated during and/or analyzed during the current study are not publicly available but are available from the corresponding author on reasonable request.

Abbreviations

FITC:

Fluoresceine isothiocyanate

MGECs:

Mouse glomerular endothelial cells

HGECs:

Human glomerular endothelial cells

HbA1c:

Hemoglobin A1c

Non-ODMIP:

Non-overt diabetes mellitus in pregnancy

ODMIP:

Overt diabetes mellitus in pregnancy

ODMIP + MAU:

Overt diabetes mellitus in pregnancy with microalbuminuria

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Funding

This study was supported by the National Natural Science Foundation of China (82000425 and 82070862).

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Author notes

  1. Y. Shu and Y. Xiong contributed equally to this work and are co-first authors.

Authors and Affiliations

  1. Department of Laboratory Medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, 39 Lake Road, East Lake Ecological Scenic, Wuhan, 430077, Hubei Province, China

    Y. Xiong & X. Bai

  2. Department of Endocrinology, Institute of Geriatric Medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, 39 Lake Road, East Lake Ecological Scenic, Wuhan, 430077, Hubei Province, China

    Y. Shu, Y. Song & S. Jin

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Contributions

X.B. conceived and designed the study. Y.S., Y.X., and X.B. carried out the experiments. X.B. analyzed the research data and wrote the manuscript. S.J. devised to establish the transcytosis model, interpreted the ROC curve data, and revised the manuscript. Y.S. and Y.X. contributed equally to this work. Y.S. and Y.X. are co-first authors. X.B. and S.J. are the guarantors of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to S. Jin or X. Bai.

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Conflict of interest

The authors have nothing to disclose. The authors declare that they have no conflicts of interest.

Ethics approval and consent to participate

This study was conducted according to the Declaration of Helsinki and was approved by Huazhong University of Science and Technology (Approval IRBID: [2022] IEC RYJ 028). Written informed consent was obtained from each patient.

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Shu, Y., Xiong, Y., Song, Y. et al. Positive association between circulating Caveolin-1 and microalbuminuria in overt diabetes mellitus in pregnancy. J Endocrinol Invest 47, 201–212 (2024). https://doi.org/10.1007/s40618-023-02137-w

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