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Association of OPG–RANKL ratio with left ventricular hypertrophy and geometric remodeling in male overweight/obese youths

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Abstract

Purpose

Receptor activator of nuclear factor kappa B ligand/receptor activator of nuclear factor kappa B/osteoprotegerin (RANKL/RANK/OPG) axis has been hypothesized as a potential mediator of left ventricular hypertrophy (LVH). The aim of the study was to assess whether circulating concentrations of RANKL, RANK, and OPG were associated with early signs of morphological cardiac changes in overweight/obese youths.

Methods

We determined serum levels of RANKL, RANK and OPG by enzyme-linked immunosorbent assays in 188 overweight/obese children and adolescents. LV mass index (LVMI) and relative wall thickness (RWT) were estimated using M-mode echocardiography.

Results

OPG and RANKL levels were higher among girls than among boys [1.73 (1.64–1.86) and 3.28 (1.90–6.37) pmol/L, respectively, vs. 1.69 (1.59–1.82) and 2.12 (1.52–3.80) pmol/L; p = 0.02 and p = 0.0001, respectively], but the OPG/RANKL ratio was lower [0.52 (0.26–0.88) vs 0.77 (0.44–1.11); p = 0.001]. In gender-specific multivariate linear regression, OPG/RANKL ratio was associated with LVMI and RWT in boys but not in girls. In multiple logistic regression, after adjustment for clinical variables, OPG/RANKL ratio was associated with concentric remodeling, eccentric and concentric LVH in boys but not in girls.

Conclusion

OPG/RANKL ratio is independently associated with LVH and patterns of LV structural remodeling in male overweight/obese children and adolescents.

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Author notes

  1. S. Zampetti and F. Lucantoni contributed equally to this paper.

Authors and Affiliations

  1. Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161, Rome, Italy

    S. Zampetti, F. Lucantoni & R. Buzzetti

  2. Policlinico Umberto I Hospital, Sapienza University of Rome, Rome, Italy

    L. Pacifico, G. Campagna & P. Versacci

  3. Institute of Translational Pharmacology, National Research Council, Rome, Italy

    P. Pierimarchi

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  1. S. Zampetti
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  2. F. Lucantoni
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Correspondence to R. Buzzetti.

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No disclosure for any prior publication or submission. No conflict of interest or role of any sponsor is present in this work. The authors alone are responsible for the content and the writing of the paper.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individuals, parents/guardians of participants included in the study.

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Zampetti, S., Lucantoni, F., Pacifico, L. et al. Association of OPG–RANKL ratio with left ventricular hypertrophy and geometric remodeling in male overweight/obese youths. J Endocrinol Invest 42, 427–434 (2019). https://doi.org/10.1007/s40618-018-0932-y

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  • DOI: https://doi.org/10.1007/s40618-018-0932-y

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