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Association between systemic leptin and neurotensin concentration in adult individuals with and without type 2 diabetes mellitus

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Abstract

Purpose

Leptin is an adipokine which regulates appetite and energy balance through a mechanism partially mediated by neurotensin (NT) in central nervous system. Besides acting as a neurotransmitter, NT is expressed in human intestine where it promotes fat absorption and its circulating levels associate with obesity, type 2 diabetes mellitus (T2DM) and cardiovascular disease. Whether a relation exists between circulating leptin and NT levels has not been investigated yet. The aim of this study was to test the hypothesis of an association between plasma leptin and NT concentration in adults with or without T2DM.

Methods

We recruited a population of 72 subjects (M/F: 39/33; age: 49.5 ± 10.6 years; BMI: 26.5 ± 4.7 kg/m2) including individuals with T2DM (n = 32) referring to our Diabetes Outpatient Clinics, Sapienza University of Rome, and healthy controls. Study participants underwent metabolic characterization; plasma leptin was measured by MILLIPLEX, Luminex, and proneurotensin (proNT), a stable precursor of NT, by chemiluminometric sandwich immunoassay.

Results

Circulating median (25°–75°) leptin levels were 2.75 (1.27–4.93) ng/mL and did not differ between T2DM and non-diabetic subjects. Leptin concentration directly correlated with proNT (r = 0.41; p = 0.015); higher leptin levels were also associated with age, male gender, obesity, higher HOMA-IR, systolic blood pressure and C-reactive protein. Belonging to the highest pro-NT quartile correlated with greater leptin levels independent of age, gender and other confounders (r2 = 0.82, p = 0.02).

Conclusions

Circulating leptin is associated with higher proNT levels independent of diabetes, obesity and metabolic syndrome components; besides its effects on central leptin signaling, NT may influence energy balance by modulating circulating leptin concentration likely through a mechanism involving gut fat absorption.

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Funding

This study was founded by Grants from Sapienza University (MGC). IB is supported by a fellowship from the “Fondazione Lilly”.

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Authors and Affiliations

  1. Department of Experimental Medicine, Section of Medical Pathophysiology, Food Science and Endocrinology, Sapienza University of Rome, Viale del Policlinico 155, 00161, Rome, Italy

    I. Barchetta, G. Ciccarelli, F. A. Cimini, V. Ceccarelli & M. G. Cavallo

  2. Department of Clinical Sciences, Lund University, Malmoe, Sweden

    M. Orho-Melander & O. Melander

Authors
  1. I. Barchetta
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  2. G. Ciccarelli
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  3. F. A. Cimini
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  4. V. Ceccarelli
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  5. M. Orho-Melander
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  6. O. Melander
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  7. M. G. Cavallo
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Corresponding author

Correspondence to M. G. Cavallo.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study protocol was reviewed and approved by the Ethics Committee of Policlinico Umberto I, Sapienza University of Rome and conducted in conformance with the Helsinki Declaration.

Informed consent

Written informed consent was obtained from all individual participants included in the study.

Data availability

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Barchetta, I., Ciccarelli, G., Cimini, F.A. et al. Association between systemic leptin and neurotensin concentration in adult individuals with and without type 2 diabetes mellitus. J Endocrinol Invest 41, 1159–1163 (2018). https://doi.org/10.1007/s40618-018-0845-9

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  • DOI: https://doi.org/10.1007/s40618-018-0845-9

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