Abstract
Introduction
High DNA polymerase β activity has been observed in the thyroid tissue of patients with Graves’ disease (Nagasaka et al. in Metabolism 37:1051–1054, 1988). This fact aroused our interest in whether the alteration of DNA polymerase β activity depends on DNA polymerase β (DNA poly β) mRNA levels, which may be modulated by thyroid-stimulating hormone (TSH) or thyroid-stimulating substances, i.e. TSH receptor antibody (TRAb).
Result
Addition of TSH or TRAb to primary cultures of Graves’ disease thyroid cells for 4 h led to no increase in DNA poly β mRNA levels. In contrast, thyroid hormone synthesizing enzyme, peroxidase, mRNA levels increased fivefold after coculture with TSH and TRAb, even though DNA poly β activity and mRNA levels are already significantly higher in Graves’ disease thyroid tissues, compared with normal thyroid tissue.
Discussion
These results indicate that DNA poly β expression in Graves’ disease thyroid cells may be maximally activated or plateau in response to thyroid-stimulating immunoglobulins, or that the activation of to poly β expression may occur via pathways other than the G protein and cyclic AMP system.
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Acknowledgements
We thank Ms. N. Takekawa, Mr. T. Tajima and Mr. Y. Mano for preparing this manuscript.
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This study is approved by the Fujita Health University Hospital Ethical Committee.
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Thyroid specimens were obtained from patients of Graves’ disease on thyroidectomy after obtaining informed consent.
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Hayakawa, N., Sato, Y., Nagasaka, A. et al. High levels of DNA polymerase β mRNA corresponding with the high activity in Graves’ thyroid tissue. J Endocrinol Invest 40, 385–389 (2017). https://doi.org/10.1007/s40618-016-0571-0
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DOI: https://doi.org/10.1007/s40618-016-0571-0