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Plasma total oxidant and antioxidant status after oral glucose tolerance and mixed meal tests in patients with polycystic ovary syndrome

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Abstract

Purpose

Insulin resistance (IR) and increased oxidative stress (OS) are the characteristics of polycystic ovary syndrome (PCOS). In this study, we aimed to evaluate the effects of oral glucose tolerance (OGTT) and mixed meal tests (MMT) on plasma total oxidant (TOS) and total antioxidant status (TAS) in patients with PCOS and the relationship between these parameters and IR, calculated via homeostasis of model assessment-IR (HOMA-IR) and Matsuda’s insulin sensitivity index (ISI) derived from OGTT and MMT.

Methods

Twenty-two patients with PCOS, and age- and body mass index (BMI)-matched 20 women as controls were enrolled into the study. Five-hour OGTT and MMT were performed on different days, and before and after these tests, plasma TOS and TAS levels were investigated. IR was calculated with HOMA-IR and Matsuda’s ISI.

Results

HOMA-IR levels were higher in patients with PCOS, compared to controls, while Matsuda’s ISI derived from OGTT and MMT was higher in controls. Plasma TOS levels before OGTT and MMT were higher in patients with PCOS than controls, while TAS levels were similar. After OGTT, plasma TOS levels became decreased at 5th hour, when compared to baseline values in PCOS group. Likewise, the same decrement was found in controls, but the decrement was not significant. After OGTT and MMT at 5th hour, no changes were observed in TAS levels, compared to baseline.

Conclusion

Matsuda’s ISIs derived from OGTT and MMT can be used instead of each other, and interestingly, we found a decrease in TOS levels after OGTT in patients with PCOS.

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Authors and Affiliations

  1. Division of Obstetrics and Gynecology, Konya Training and Research Hospital, Konya, Turkey

    Zehra Kucukaydın, Mustafa Basaran & Ahmet Basaran

  2. Division of Endocrinology and Internal Medicine, Division of Endocrinology and Metabolism, Konya Training and Research Hospital, Meram Yeniyol, 42100, Meram, Konya, Turkey

    Cevdet Duran

  3. Ermenek State Hospital, Ermenek, Karaman, Turkey

    Fatos Camlica

  4. Division of Biochemistry, Konya Training and Research Hospital, Konya, Turkey

    Sami Said Erdem

  5. Division of Internal Medicine, Okmeydani Training and Research Hospital, Istanbul, Turkey

    Orkide Kutlu

  6. Division of Endocrinology and Internal Medicine, Konya Training and Research Hospital, Konya, Turkey

    Ferda Sevimli Burnik

  7. Division of Internal Medicine, Konya Training and Research Hospital, Konya, Turkey

    Halis Elmas

  8. Division of Endocrinology and Internal Medicine, Medical Faculty, Istanbul Bilim University, Istanbul, Turkey

    Mustafa Sait Gonen

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  1. Zehra Kucukaydın
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  2. Cevdet Duran
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Correspondence to Cevdet Duran.

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This study was supported by Konya Training and Research Hospital’s Research Fund.

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Authors declare that they have no conflict of interest.

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All procedures in the study involving human participants were performed in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Kucukaydın, Z., Duran, C., Basaran, M. et al. Plasma total oxidant and antioxidant status after oral glucose tolerance and mixed meal tests in patients with polycystic ovary syndrome. J Endocrinol Invest 39, 1139–1148 (2016). https://doi.org/10.1007/s40618-016-0498-5

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  • DOI: https://doi.org/10.1007/s40618-016-0498-5

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