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Assessment of human lymphocyte proliferation associated with metabolic syndrome

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Abstract

Background

Metabolic syndrome (MetS), a cluster of various metabolic conditions, has become epidemic and causes increased morbidity and mortality.

Purpose

The aim of this study was to compare lymphocyte proliferation under two different stimuli, Concanavalin A (ConA) and insulin, in a group of patients with MetS (Group 1) and a healthy group (Group 2).

Methods

Group 1 consisted of 53 patients who met the diagnostic criteria for MetS. Group 2 consisted of 63 patients without MetS. All individuals were evaluated for lipid profile and glycemia. Lymphocyte extraction and culture were performed for each subject and lymphocyte proliferation was assessed using the Alamar blue technique.

Results

There was no gender difference between both groups, but in terms of age, there was a significant difference. The use of Con A at concentrations of 1 and 5 µg/mL induced a high lymphocyte proliferation in both groups. In contrast, when different concentrations of insulin were added, no significant changes in lymphocyte proliferation were observed. However, the proliferation of lymphocytes was significantly higher in Group 1 compared to Group 2 under insulin stimulus, which did not happen under ConA stimulation. Even after age and gender correction, this difference was maintained.

Conclusions

The increased lymphocyte proliferative response to insulin in patients with MetS found in this study suggests a role of the lymphocyte response to insulin in the pathophysiology of MetS. This response may be used as an immuno-biological marker for MetS, although further studies to evaluate its clinical usefulness need to be conducted.

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Acknowledgments

The authors acknowledge financial support from Universidad Tecnológica de Pereira (Grant 5-11-12, Pereira, Colombia) and COLCIENCIAS (Contrato 220-2010, Bogotá, Colombia).

Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical approval

This study was approved by the Bioethics Committee of the Universidad Tecnológica de Pereira. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation at Universidad Tecnológica de Pereira.

Informed consent

A written informed consent was obtained from each participant in this study.

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Authors and Affiliations

  1. Faculty of Health Sciences, Universidad Tecnológica de Pereira, A.A. 97, La Julita, Pereira, Risaralda, Colombia

    O. A. Pinzón, J. C. Sánchez, J. C. Sepúlveda-Arias & D. F. López-Zapata

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  1. O. A. Pinzón
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Correspondence to O. A. Pinzón.

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Pinzón, O.A., Sánchez, J.C., Sepúlveda-Arias, J.C. et al. Assessment of human lymphocyte proliferation associated with metabolic syndrome. J Endocrinol Invest 38, 1277–1282 (2015). https://doi.org/10.1007/s40618-015-0307-6

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  • DOI: https://doi.org/10.1007/s40618-015-0307-6

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