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Herpesvirus DNA Polymerase Mutants—How Important Is Faithful Genome Replication?

  • Virology (A Nicola, Section Editor)
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A Correction to this article was published on 27 January 2020

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Abstract

Purpose of Review

This report broaches the topic of altered fidelity of DNA replication in herpesvirus mutants described over the past decades. Reduced genome replication fidelity of herpesvirus exonuclease mutants allows studying of virus population dynamics in the absence of exonucleolytic proofreading and can inform us on virus evolution in the face of error-prone genome replication.

Recent Findings

We recently found that mutations previously described to be lethal for herpes simplex type 1 (HSV-1) caused error-prone genome replication in Marek’s disease virus. This has allowed us to study the influence of augmented genetic diversity on viral population dynamics, replicative fitness, and virulence.

Summary

We conclude that the use of herpesvirus fidelity mutants allows unprecedented insights into virus evolution driven by low-fidelity replication. More than that, their use allows us to observe accelerated evolution, potentially enabling time-saving screens for the rise of drug- or vaccine-resistant mutants. In addition, we can infer that lethal or suicidal phenotypes observed in low-fidelity herpesvirus mutants are likely a consequence of error-prone genome replication, ultimately leading to lethal mutagenesis of small and isolated virus populations in cell culture.

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Change history

  • 27 January 2020

    During the production process, the documents provided by the authors were inaccurately merged. As a result all 15 references provided in the tables of the paper are wrong.

  • 27 January 2020

    During the production process, the documents provided by the authors were inaccurately merged. As a result all 15 references provided in the tables of the paper are wrong.

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  1. Institut für Virologie, Robert von Ostertag-Haus, Zentrum für Infektionsmedizin, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163, Berlin, Germany

    Jakob Trimpert & Nikolaus Osterrieder

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The original version of this article was revised: During the production process, the documents provided by the authors were inaccurately merged. As a result all 15 references provided in the tables of the paper are wrong. The authors identified the error during the proof stage; however, the requested changes were not incorporated in the final published version. Likewise, some other corrections requested by the authors in the proof process remained unchanged in the published version of the manuscript. The publisher apologizes and takes full responsibility for the problems that result in the changes to this article.

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Trimpert, J., Osterrieder, N. Herpesvirus DNA Polymerase Mutants—How Important Is Faithful Genome Replication?. Curr Clin Micro Rpt 6, 240–248 (2019). https://doi.org/10.1007/s40588-019-00135-2

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