Purpose of Review
Growth-enhancing chemicals used by the beef and dairy industries may be bioavailable to humans via milk, meat, and other environmental matrices. This review evaluates the potential for environmental transport and bioavailability of the active chemical to humans.
Bovine somatostatin is detectable in milk; however, there is no evidence that the protein persists in the environment nor that it is active in humans. In contrast, steroids are transported through milk and meat to humans where they may exert biological activity. Furthermore, environmental matrices such as raw water and dust may also allow for the environmental transport and bioavailability of steroids to humans.
Endogenous and exogenous steroids can be found in the meat, milk, and waste materials produced by cattle. While the concentrations may be low, exposure to these matrices, most notably dairy products made with whole milk, can be a source of exogenous steroids to humans.
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Haug A, Hostmark AT, Harstad OM. Bovine milk in human nutrition—a review. Lipids Health Disease. 2007;6:25.
US Department of Health and Human Services. (2017) Foods linked to illness. https://www.cdc.gov/foodsafety/foods-linked-illness.html. Last accessed, March 5, 2018.
US Department of Health and Human Services (2012) Foodborne illnesses. NIH Publication No.-12–4730. Available at: www.digestive.niddk.nih.gov. Last accessed, March 5, 2018.
Morbidity CDC, Report MW. Human tuberculosis caused by Mycobacterium bovis—New York City, 2001-2004. June 24. 2005;54(24):605–8.
Kolok AS, Sellin MK. The environmental impact of growth-promoting compounds employed by the United States beef cattle industry: history, current knowledge, and future directions. In: Reviews of environmental contamination and toxicology, vol. 195; 2008. p. 1–30.
Jones SJ, Johnson RD, Calkins CR, Dikeman ME. Effects of trenbolone acetate on carcass characteristics and serum testosterone in bulls and steers on different management and implant schemes. J Anim Sci. 1991;69:1363–9.
Lone KP. Natural sex steroids and the xenobiotic analogs in animal production: growth, carcass quality, pharmacokinetics, metabolism, mode of action, residues, methods and epidemiology. Crit Rev Food Sci. 1997;37:93–209.
Rumsey TS, Tyrrell HF, Dinius DA, Moe PW, Cross HR. Effect of diethylstilbestrol on tissue gain and carcass merit of feedlot beef steers. J Anim Sci. 1981;53:589–600.
Herbst AL, Ulfelder H, Poskanzer DC. Adenocarcinomas of the vagina: association of maternal stilbestrol therapy with tumor appearance in young women. N Engl J Med. 1971;284:878–81.
Wade N. DES: a case of regulatory abdication. Science. 1972;177:335–7.
Epstein SS. The chemical jungle: today’s beef industry. Int J Health Sci. 1990;20:277–80.
Butler LJ. The profitablility of rBST on U.S. dairy farms. AgBioforum. 1999;2(2):111–7.
Taur et al. The effect of bovine somatotropin on the cost of producing milk: estimates using propensity scores. J Dairy Sci. 2016;99(4):2979–85. https://doi.org/10.3168/jds.2015-9942.
U.S. Department of Agriculture. (2008) Dairy. Part III: reference of dairy cattle health and management practices in the United States, 2007 USDA–APHIS–VS, CEAH. Fort Collins. CO #N482. 2007:0908.
USDA. 2016. Dairy 2014, “Dairy Cattle Management Practices in the United States, 2014” USDA–APHIS–VS–CEAH–NAHMS. Fort Collins, CO #692.0216.
American Cancer Society. (2017) Recombinant bovine growth hormone. https://www.cancer.org/cancer/cancer-causes/recombinant-bovine-growth-hormone.html. Last accessed, December 2, 2017
Bauman DE. Bovine somatotropin: review of an emerging animal technology. J Dairy Sci. 1992;75(12):3432–51. https://doi.org/10.3168/jds.S0022-0302(92)78119-3.
Parodi PW. Dairy product consumption and the risk of prostate cancer. Int Dairy J. 2009;19(10):551–65. https://doi.org/10.1016/j.idairyj.2009.05.006.
Sejrsen K, Pedersen LO, Vestergaard M, Purup S. Biological activity of bovine milk contribution of igf-i and igf binding proteins. Livest Prod Sci. 2001;70(1–2):79–85. https://doi.org/10.1016/S0301-6226(01)00199-3.
Meyer Z, Höflich C, Wirthgen E, Olm S, Hammon HM, Hoeflich A. Analysis of the IGF-system in milk from farm animals—occurrence, regulation, and biomarker potential. Growth Hormone and IGF Research. 2017;35:1–7. https://doi.org/10.1016/j.ghir.2017.05.004.
Collier RJ, Bauman DE. Update on human health concerns of recombinant bovine somatotropin use in dairy cows. J Anim Sci. 2014;92(4):1800–7. https://doi.org/10.2527/jas2013-7383.
Lampe JW. Dairy products and cancer. J Am Coll Nutr. 2011;30:464S–70S. https://doi.org/10.1080/07315724.2011.10719991.
Larsson SC, Crippa A, Orsini N, Wolk A, Michaëlsson K. Milk consumption and mortality from all causes, cardiovascular disease, and cancer: a systematic review and meta-analysis. Nutrients. 2015;7(9):7749–63. https://doi.org/10.3390/nu7095363.
Thorning TK, Raben A, Tholstrup T, Soedamah-Muthu SS, Givens I, Astrup A. Milk and dairy products: good or bad for human health? An assessment of the totality of scientific evidence. Food and Nutrition Research. 2016;60:32527. https://doi.org/10.3402/fnr.v60.32527.
Rowlands M, Gunnell D, Harris R, Vatten LJ, Holly JMP, Martin RM. Circulating insulin-like growth factor peptides and prostate cancer risk: a systematic review and meta-analysis. Int J Cancer. 2009;124(10):2416–29. https://doi.org/10.1002/ijc.24202.
• Harrison S, Lennon R, Holly J, Higgins JPT, Gardner M, Perks C, et al. Does milk intake promote prostate cancer initiation or progression via effects on insulin-like growth factors (IGFs)? A systematic review and meta-analysis. Cancer Causes and Control. 2017;28(6):497–528. https://doi.org/10.1007/s10552-017-0883-1. The study conducted a meta-analysis concluding that IGF-I is a potential mechanism underlying the observed associations between milk intake and prostate cancer risk.
Qin L, Wang P, Kaneko T, Hoshi K, Sato A. Estrogen: one of the risk factors in milk for prostate cancer. Med Hypotheses. 2004;62(1):133–42. https://doi.org/10.1016/S0306-9877(03)00295-0.
Hartmann S, Lacorn M, Steinhart H. Natural occurrence of steroid hormones in food. Food Chem. 1998;62:7–20.
Yu C, Deeb RA, Chu K. Microbial degradation of steroidal estrogens. Chemosphere. 2013;91(9):1225–35. https://doi.org/10.1016/j.chemosphere.2013.01.112.
Fritsche S, Rumsey TS, Meyer HHD, Schmidt G, Steinhart H. Profiles of steroid hormones in beef from steers implanted with Synovex-S (estradiol benzoate and progesterone) in comparison to control steers. Z Lebensm Unters Forsch A. 1999;208:328–31.
Courant F, Antignac J, Laille J, Monteau F, Andre F, Le Bizec B. Exposure assessment of prepubertal children to steroid endocrine disruptors. 2. Determination of steroid hormones in milk, egg, and meat samples. J Agric Food Chem. 2008;56(9):3176–84. https://doi.org/10.1021/jf800096f.
Adamusova H, Bosakova Z, Coufal P, Pacakova V. Analysis of estrogens and estrogen mimics in edible matrices—a review. J Sep Sci. 2014;37(8):885–905. https://doi.org/10.1002/jssc.201301234.
Goyon A, Cai JZ, Kraehenbuehl K, Hartmann C, Shao B, Mottier P. Determination of steroid hormones in bovine milk by LC-MS/MS and their levels in Swiss Holstein cow milk. Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment. 2016;33(5):804–16.
Xu L, Zhang L, Zhang Y, Sheng Q, Zhao A. Qualitative and quantitative comparison of hormone contents between bovine and human colostrums. Int Dairy J. 2011;21(1):54–7. https://doi.org/10.1016/j.idairyj.2010.04.006.
Macrina AL, Ott TL, Roberts RF, Kensinger RS. Estrone and estrone sulfate concentrations in milk and milk fractions. J Acad Nutr Diet. 2012;112(7):1088–93. https://doi.org/10.1016/j.jand.2012.02.005.
Yang Y, Shao B, Zhang J, Wu Y, Duan H. Determination of the residues of 50 anabolic hormones in muscle, milk and liver by very-high-pressure liquid chromatography-electrospray ionization tandem mass spectrometry. J Chromatogr B Anal Technol Biomed Life Sci. 2009;877(5–6):489–96. https://doi.org/10.1016/j.jchromb.2008.12.054.
Vandenberg, L. N., Colborn, T., Hayes, T. B., Heindel, J. J., Jacobs, D. R., Lee, D. -., . . . Myers, J. P. (2012) Hormones and endocrine-disrupting chemicals: low-dose effects and nonmonotonic dose responses. Endocr Rev, 33(3), 378–455. doi:https://doi.org/10.1210/er.2011-1050.
U.S. Department of Health and Human Services and U.S. Department of Agriculture. 2015 – 2020 dietary guidelines for Americans. In: 8th edition; 2015. https://health.gov/dietaryguidelines/2015/guidelines/.
Archer, D. F., Nakajima, S. T., Sawyer, A. T., Wentworth, J., Trupin, S., Koltun, W. D., . . . Ellman, H. (2013) Norethindrone acetate 1.0 milligram and ethinyl estradiol 10 micrograms as an ultra low-dose oral contraceptive. Obstet Gynecol, 122(3), 601–607. doi:https://doi.org/10.1097/AOG.0b013e3182a1741c.
Hammond GL. Plasma steroid-binding proteins: primary gatekeepers of steroid hormone action. J Endocrinol. 2016;230(1):R13–25. https://doi.org/10.1530/JOE-16-0070.
U.S. Food and Drug Administration. (2017) Steroid hormone implants used for growth in food-producing animals. October 2017. https://www.fda.gov/AnimalVeterinary/SafetyHealth/ProductSafetyInformation/ucm055436.htm Last Accessed: December 1, 2017.
IARC, (1987) Overall evaluations of carcinogenicity: an updating of IARC Monographs Volumes 1 to 42. Supplement No 7. International Agency for Research on Cancer, Lyon.
IARC, (1995) Monographs on the Evaluation of the carcinogenic risks to humans. Sex Hormones 21, International Agency for Research on Cancer, Lyon.
Lipschutz A, Vargas L Jr. Structure and origin of uterine and extragenital fibroids induced experimentally in the guinea pig by prolonged administration of estrogens. Cancer Res. 1994;1:236–48.
Perez-Comas A. Premature sexual development in Puerto Rico. Boletin Asociacion Medicade Puerto Rico. 1988;80:85–90.
Saenz de Rodriguez CA, Bongiovanni AM, Conde de Borrego L. An epidemic of precocious development in Puerto Rican children. J Pediatr. 1985;107:393–6.
Fara, G.M., De. Corvo, G., Bernuzzi, S., Bigatello, A., Di Pietro, C., Scaglioni, S., Chiumello, G. Epidemic of breast enlargement in an Italian school. Lancet. August. 1979;11:295–7.
Pasquino AM, Balducci R, Manca Bitti ML, Spadoni GL, Boscherini B. Transient pseudo-precocious puberty by probably oestrogen intake in 3 girls. Arch Dis Child. 1982;57:954–6.
Abete I, Romaguera D, Vieira AR, Lopez de Munain A, Norat T. Association between total, processed, red and white meat consumption and all cause, CVD and IHD mortality: a meta-analysis of cohort studies. Britian Journal of Nutrition. 2014;112:762–75.
• Lippi, G., C. Mattiuzzi, G. Cervellin. (2016) Meat consumption and cancer risk: a critical review of published meta-analyses critical reviews in oncology/hematology 97: 1–14. This study conducted a meta-analysis that found an association between beef consumption and cancer.
US Census of Agriculture. 2012. Available at: https://www.agcensus.usda.gov/Publications/2012/ Last accessed, March 5, 2018.
Barker, J.C. and F.R. Walls. 2002. Livestock manure productin rates and nutrient content. 2002 North Carolina Agricultural Chemicals Manual. Available at: (http://agrienvarchive.ca/bioenergy/download/barker_ncsu_manure_02.pdf). Last accessed, March 5, 2018.
Bartelt-Hunt SL, Snow DD, Kranz WL, Mader TL, Shapiro CA, van Donk SJ, et al. Effect of growth promotants on the occurrence of natural and synthetic steroid hormones on feedlot soils and in runoff from beef cattle feeding operations. Environ Sci Technol. 2012;46(3):1352–60.
Schiffer B, Daxenberger A, Meyer K, Meyer HHD. The fate of Trenbolone acetate and Melengesterol acetate as growth promoters in cattle: environmental studies. Environ Health Perspect. 2001;109:1145–51.
Noguera-Oviedo KM, Aga DS. Chemical and biological assessment of endocrine disrupting chemicals in a full scale dairy manure anaerobic digester with thermal pretreatment. Sci Total Environ. 2016;550:827–34.
Zheng W, Yates SR, Bradford SA. Analysis of steroid hormones in a typical dairy waste disposal system. Environ Sci Technol. 2008;42:530–5.
Havens SM, Hedman CJ, Hemming JDC, Miertz MG, Shafer MM, Schauer JJ. Stability, preservation and quantification of hormones and estrogenic and androgenic activities in surface water runoff. Environ Toxicol Chem. 2010;29:2481–90.
Kolodziej EP, Harter T, Sedlak DL. Dairy wastewater, aquaculture and spawning fish as sources of steroid hormones in the aquatic environment. Environ Sci Technol. 2004;38:6377–84.
Lorenzen A, Hendel JG, Conn KL, Bittman S, Swabiah AB, Lazarovitz G, et al. Survey of hormone activities in municipal biosolids and animal manures. Environ Toxicol. 2004;19:216–25.
Sangster JL, Ali JM, Snow DD, Kolok AS, Bartelt-Hunt SL. Bioavailability and fate of sediment-associated progesterone in aquatic systems. Environ Sci Technol. 2016;50(7):4027–36.
CDC. 2014. Drinking water. Private ground water wells. https://www.cdc.gov/healthywater/drinking/private/wells/index.html). Last Accessed, March 5, 2018.
Arnon S, Dahan O, Elhanany S, Cohen K, Pankratov I, Gross A, et al. Transport of testosterone and estrogen from dairy-farm waste lagoons to groundwater. Environ Sci Technol. 2008;42:5521–6.
Kolodziej EP, Sedlak DL. Rangeland grazing as a source of steroid hormones to surface waters. Environ Sci Technol. 2007;41:3514–20.
Bartelt-Hunt SL, Snow DD, Damon-Powell TR, Meisbach D. Occurrence of steroid hormones and antibiotics in shallow groundwater impacted by livestock waste control facilities. J Contam Hydrol. 2011;123:94–103.
•• Blackwell, B.R., K.J. Wooten, M.D. Buser, B.J. Johnson, G.P. Cobb, and P.N. Smith. (2015) Occurrence and characterization of steroid growth promoters associated with particulate matter originating from beef cattle feedyards. Environmental science and technology. Environ. Sci. Technol., 2015, 49 (14), pp 8796–8803. This study provides evidence that dusts blown from feedlot operations contain steroidal growth promoting compounds.
Wooten KJ, Blackwell BR, McEachran AD, Mayer GD, Smith PN. Airborne particulate matter collected near beef cattle feedyards induces androgenic and estrogenic activity in vitro. Agric Ecosyst Environ. 2015;203:29–35. https://doi.org/10.1016/j.agee.2015.01.016.
Kirkhorn SR, Garry VF. Agricultural lung diseases. Environ Health Perspect. 2000;108(Suppl. 4):705–12.
Conflict of Interest
Alan S. Kolok, Jonathan M. Ali, Eleanor G. Rogan, and Shannon L. Bartelt-Hunt declare that they have no conflict of interest.
Human and Animal Rights and Informed Consent
This article does not contain any studies with human or animal subjects performed by any of the authors.
This article is part of the Topical Collection on Food, Health, and the Environment
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Kolok, A.S., Ali, J.M., Rogan, E.G. et al. The Fate of Synthetic and Endogenous Hormones Used in the US Beef and Dairy Industries and the Potential for Human Exposure. Curr Envir Health Rpt 5, 225–232 (2018). https://doi.org/10.1007/s40572-018-0197-9
- Exogenous hormones