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Dynamic changes in p66Shc mRNA expression in peripheral blood mononuclear cells following resistance training intervention in old frail women born to obese mothers: a pilot study

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Abstract

The p66Shc gerontogene may affect healthspan by promoting fat accumulation. We assessed changes of p66Shc-mRNA in peripheral tissues in relation to maternal obesity and the moderating effects of resistance-training (RT) exercise in elderly frail women. Thirty-seven women participated in a 4-month RT program. Twenty were offspring of lean/normal weight mothers and 17 were offspring of overweight/obese mothers (OOM). P66Shc was assessed in peripheral blood mononuclear cells (PBMC) and in subcutaneous adipose tissue (SAT) before and after RT. Overall, OOM showed elevated p66Shc mRNA levels in the PBMC. Independently from maternal obesity, following RT there was a decrease in p66Shc expression in PBMC but not in SAT, particularly in subjects with a high body mass index. Results suggest that maternal obesity has long-term effects on the expression of genes involved in mitochondrial function and fat deposition and that RT modifies p66Shc expression in PBMC with greater effects in obese subjects.

ClinicalTrials.gov ID: NCT01931540.

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Acknowledgements

The study was conducted within the EU-funded project “Developmental Origins of Healthy and Unhealthy Ageing: The Role of Maternal Obesity” (FP7-HEALTH-DORIAN, G.A. 278603), and the Finnish Centre of Excellence in Molecular Imaging in Cardiovascular and Metabolic Research, supported by the Academy of Finland, University of Turku, Turku University Hospital and Åbo Akademi University. We thank M. Trinei, M. Giorgio and P.G. Pelicci at European Institute for Oncology, Milan, Italy, for providing the p66Shc primers and Silvia Poggini for graphical help.

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Authors and Affiliations

  1. Center for Behavioral Sciences and Mental Health, Istituto Superiore di Sanità, Rome, Italy

    Alessandra Berry, Carla Raggi & Francesca Cirulli

  2. Turku PET Centre, University of Turku, Turku, Finland

    Marco Bucci, Pirjo Nuutila & Ville Huovinen

  3. Institute of Clinical Physiology, National Research Council, Pisa, Italy

    Maria Angela Guzzardi & Patricia Iozzo

  4. Department of Chronic Disease Prevention, National Institute of Health and Welfare, Helsinki, Finland

    Johan G. Eriksson

  5. Folkhälsan Research Center, Helsinki, Finland

    Johan G. Eriksson

  6. Department of General Practice and Primary Health Care, University of Helsinki and Helsinki University Hospital, Helsinki, Finland

    Johan G. Eriksson

  7. Department of Endocrinology, Turku University Hospital, Turku, Finland

    Pirjo Nuutila

  8. Department of Radiology, University of Turku, Turku, Finland

    Ville Huovinen

  9. Department of Radiology, Turku University Hospital, Turku, Finland

    Ville Huovinen

Authors
  1. Alessandra Berry
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  2. Marco Bucci
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  3. Carla Raggi
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  4. Johan G. Eriksson
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  5. Maria Angela Guzzardi
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  6. Pirjo Nuutila
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  7. Ville Huovinen
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  8. Patricia Iozzo
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  9. Francesca Cirulli
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Contributions

AB analyzed all p66Shc data, conducted the statistical analysis, interpreted the results and drafted the article; MB and VH collected PBMC and SAT samples and drafted the article CR analyzed all PBMC and SAT samples; MAG helped with interpretation of the data and drafted the article; FC, PI, JGE and PN made substantial contributions to the conception, design and coordination of the study and to interpretation of the data, and drafted the article. All authors revised the article critically for important intellectual content and approved the final version to be published. AB and FC are the guarantors of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.

Corresponding author

Correspondence to Alessandra Berry.

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Funding

European Commission under the FP7 (Project DORIAN—Developmental ORIgins of healthy and unhealthy AgeiNg: The role of maternal obesity—Grant Agreement no. 278603) and H2020 AwE “Ageing with Elegans” (Grant Agreement no. 633589).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study was conducted according to the principles of Declaration of Helsinki 1964 and and its later amendments and was approved by the Ethics Committee of the Hospital District of South-West Finland.

Informed consent

All participants gave written informed consent.

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Berry, A., Bucci, M., Raggi, C. et al. Dynamic changes in p66Shc mRNA expression in peripheral blood mononuclear cells following resistance training intervention in old frail women born to obese mothers: a pilot study. Aging Clin Exp Res 30, 871–876 (2018). https://doi.org/10.1007/s40520-017-0834-4

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  • DOI: https://doi.org/10.1007/s40520-017-0834-4

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