Abstract
In the present study, the rubisco protein was evaluated as an effective air pollution stress indicator in plant species such as Mangifera indica L. and Azadirachta indica A. Juss. that grown near lignite based thermal power station, southern India. The plant species were used as biomarkers at three different field locations in downwind areas receiving significant level of air pollution. The results entail that respirable suspended particulate matter, suspended particulate matter, sulphur dioxide and nitrogen dioxide are the major air pollutants found in the study area. The expression pattern of rubisco protein in A. indica was significantly reduced at experimental site followed by confirmation with western blot analysis. This is a first kind of study to analyse the air pollution stress changes using rubisco enzyme in plants at natural environmental conditions. The results from this study would pave future perspectives to engineer rubisco for assimilating of carbon dioxide.
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References
Agarwal, M (1985). Plant factor as indicator of SO2 and O3 pollutants: In Proceedings of international symposium on biological monitoring of the state of environment (Bio-indicators) (pp. 225–231). Indian National Science Academy: New Delhi.
Agarwal, G. K., Rakwal, R., Yonekura, M., Kubo, A., & Saji, H. (2002). Proteome analysis of differentially displayed proteins as a tool for investigating ozone stress in rice (Oryza sativa L.) seedlings. Proteomics, 2(8), 947–959.
Backett, K. P., Free-smith, P. H., & Taylor, G. (1998). Urban woodlands: Their role in reducing the effect of particulate pollution. Environmental Pollution, 99(3), 306–347.
Banerjee, S., Singh, A. K., & Banerjee, S. K. (2003). Impact of fly ash on foliar chemical and biochemical composition of naturally occurring ground flora and its possible utilization for growing tree crop. Indian Forester, 129, 964–977.
Brendley, B. W., & Pell, E. J. (1998). Ozone-induced changes in biosynthesis of rubisco and associated compensation to stress in foliage of hybrid poplar. Tree Physiology, 18(2), 81–90.
Chaudhari, P. R., & Gajghate, D. G. (2000). Assessment of air pollution effect on plants—a review. Indian Journal of Environmental Protection, 20(12), 925–933.
Chen, Z., Warren, C. R., & Adams, A. A. (2000). Separation of rubisco in extracts of plant leaves by capillary electrophoresis with sieving polymers. Analytical Letters, 33(4), 579–587.
CMRI. (1998). Determination of emission factor for various opencast mining activities, in Report GAP/9/EMG/MOEF/97. Dhanbad: Central Mining Research Institute, Environmental Management Group.
Crabbe, H., Beaumont, R., & Norton, D. (2000). Assessment of air quality, emissions and management in a local urban environment. Urban Air Quality: Measurement, Modelling and Management, 65, 435–442.
Davies, K. J. A., Lin, S. W., & Pacifici, R. E. (1987). Protein damage and degradation by oxygen radicals. IV. Degradation of denatured protein. The Journal of Biological Chemistry, 262(20), 9914–9920.
Desimone, M., Henke, A., & Wagner, E. (1996). Oxidative stress induces partial degradation of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase in isolated chloroplasts of barley. Plant Physiology, 111(3), 789–796.
Dizengremel, P. (2001). Effects of ozone on the carbon metabolism of forest trees. Plant Physiology and Biochemistry, 39(9), 729–742.
Eckardt, N. A., & Pell, E. J. (1995). Oxidative modification of Rubisco from potato foliage in response to ozone. Plant Physiology Biochemistry, 33(3), 273–282.
Freedman, B. (1989). Environmental ecology: The impacts of pollution and other stresses on ecosystem structure and function. In P. Bonnett (Ed.), Land degradation & development (pp. 325–326). San Diego: Academic Press.
Govindaraju, M., Ganeshkumar, R. S., Muthukumaran, V. R., & Visvanathan, P. (2011). Identification and evaluation of air- pollution-tolerant plants around lignite—based thermal power station for greenbelt development. Environmental Science Pollution Research, 19(4), 1210–1223.
Ishida, H., Nishimori, Y., Sugisawa, M., Makino, A., & Mae, T. (1997). The large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is fragmented into 37-kDa and 16-kDa polypeptides by active oxygen in the lysates of chloroplasts from primary leaves of wheat. Plant Cell Physiology, 38(4), 471–479.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.
Nakamura, H., & Saka, H. (1978). Photochemical oxidants injury in rice plants. III. Effect of ozone on physiological activities in rice plants. Japanese Journal of Crop Sciences, 47, 704–714.
Nanda, S. N., & Tiwary, S. N. (2001). Concentration of SPM in the Burla–Hirakud–Sambalpur region (Orissa). Indian Journal of Environmental Protection, 21(3), 193–202.
Pell, E. J. (1979). How air pollutants induce disease. In J. G. Horsfall & C. B. Cowling (Eds.), Plant disease (pp. 273–292). New York: Academic Press.
Reddy, G., Arteca, R. N., Dai, Y. R., Flores, H. E., Negm, F. B., & Pell, E. J. (1993). Changes in ethylene and polyamines in relation to mRNA levels of the large and small subunits of ribulose bisphosphate carboxylase/oxygenase in ozone-stressed potato foliage. Plant, Cell and Environment, 16(7), 819–826.
Sharma, A. P., & Tripathi, B. D. (2008). Assessment of atmospheric PAHs profile through Calotropis gigantean R.Br leaves in the vicinity of an Indian coal-fired power plant. Environmental Monitoring Assessment, 149(1–4), 477–482.
Tabita, F. R. (2007). Rubisco: the enzyme that keeps on giving. Cell, 129(6), 1039–1040.
Thompson, C. R., Farrah, G. H., Haff, L. V., Hillman, W. S., Hook, A. W., Schneider, E. J., et al. (1997). Tentative method of analysis for floride content of the atmosphere and plant tisues. In M. Katz (Ed.), Methods for air sampling and analysis (pp. 417–426). Washington, DC: American Public Health Association.
Towbin, H., Staehelin, T., & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proceedings of the National Academy of Sciences of United States of America., 76, 4350–4354.
Visioli, G., Marmiroli, M., & Marmiroli, N. (2010). Two-dimensional liquid chromatography technique coupled with mass spectrometry analysis to compare the proteomic response to cadmium stress in plants. Journal of Biomedicine Biotechnology. https://doi.org/10.1155/2010/567510.
Wheeler, A. J., Williams, I., Beaumont, R. A., & Manilton, R. S. (2000). Characterisation of particulatematter sampled during a study of children’s personal exposure to air borne particulate matter in a UK urban environment. Environmental Monitoring Assessment, 65(1–2), 69–77.
Wong, S. C. (1979). Elevated atmospheric partial pressure of CO2 and plant growth. Interactions of nitrogen nutrition and photosynthetic capacity in C3 and C4 plants. Oecologia, 44, 68–74.
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The authors are thankful to DST-CO2 Sequestration Division, UGC-SAP and DST-FIST, New Delhi for their financial assistance to carry out the research work.
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Govindaraju, M., Ganeshkumar, R.S., Suganthi, K. et al. Functional analysis of rubisco protein in selected plants grown under air pollution stress environment. Ind J Plant Physiol. 23, 201–208 (2018). https://doi.org/10.1007/s40502-018-0372-x
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DOI: https://doi.org/10.1007/s40502-018-0372-x