Indian Journal of Plant Physiology

, Volume 23, Issue 1, pp 48–56 | Cite as

Comparison of biochemical and molecular responses of two Brassica napus L. cultivars differing in drought tolerance to salt stress

  • Fatemeh Rahmani
  • Arghavan Peymani
  • Abdollah Hassanzadeh Gorttapeh
Original Article


In this study, levels of MDA, total protein, soluble sugars, and enzymes activity including POX, APX, and CAT were measured in 12 dSm−1 NaCl treated seedlings belong to Licord (drought sensitive cultivar) and SLM046 (drought tolerant cultivar) of Brassica napus. The results showed that salinity increased the amount of MDA, soluble sugars and total protein in both cultivars. Two cultivars partially displayed different trend concerning enzymatic activities under salt stress experiment. Moreover, transcript abundance of four genes involved in signal transduction pathway including Auxin responsive protein, Protein kinase, MAPK3 and MAPK4 were explored at 0, 3, 6, 12 and 24 h under 12 dSm−1 NaCl treatment using RT-PCR approach. The molecular analyses of Licord cultivar revealed the lowest accumulation of all genes after 6 h exposure to NaCl except MAPK3 which was detected at the highest level at this time point. Molecular results of SLM046 cultivar showed that maximum expression of all genes occurred after 6 h treatment except MAPK3 which showed the lowest transcript at 6 h. Our studies indicate better response of SLM046 cultivar to salinity condition compared to Licord cultivar.


Brassica napus L. Biochemical parameters Gene expression Molecular parameters Salinity 



Authors are also thankful to Biotechnology Research Center of Urmia University for technical support of this work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Abdul Qados, A. M. S. (2011). Effect of salt stress on plant growth and metabolism of bean plant Vicia faba (L.). Journal of the Saudi Society of Agricultural Sciences, 10, 7–15.CrossRefGoogle Scholar
  2. Aebi, H. (1984). Catalase in vitro. In L. Packer (Ed.), Methods in enzymology (pp. 121–126). San Diego, CA: Academic Press Inc.Google Scholar
  3. Ashraf, M., & Harris, P. J. C. (2004). Potential biochemical indicators of salinity tolerance in plants. Plant Science, 166, 3–16.CrossRefGoogle Scholar
  4. Ashraf, M., & McNeilly, T. (2004). Salinity tolerance in some Brassica oilseeds. Critical Reviews in Plant Sciences, 23, 157–174.CrossRefGoogle Scholar
  5. Azevedo Neto, A. D., Prico, J. T., Eneas-Filho, J., Braga de Abreu, C. E., & Gomes-Filho, E. (2006). Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environmental and Experimental Botany, 56, 87–94.CrossRefGoogle Scholar
  6. Borsani, O., Valpuesta, V., & Botella, M. A. (2001). Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. Plant Physiology, 126, 1024–1030.CrossRefPubMedPubMedCentralGoogle Scholar
  7. Fan, Y., Shabala, S., Ma, Y., Xu, R., & Zhou, M. (2015). Using QTL mapping to investigate the relationships between abiotic stress tolerance (drought and salinity) and agronomic and physiological traits. BMC Genomics, 16, 43.CrossRefPubMedPubMedCentralGoogle Scholar
  8. Flowers, T. J. (2004). Improving crop salt tolerance. Journal of Experimental Botany, 55, 307–319.CrossRefPubMedGoogle Scholar
  9. Gueta-Dahan, Y., Yaniv, Z., Zilinskas, B. A., & Ben-Hayyim, G. (1997). Salt and oxidative stress: Similar and specific responses and their relation to salt tolerance in citrus. Planta, 203, 460–469.CrossRefPubMedGoogle Scholar
  10. Heath, R. L., & Packer, L. (1968). Photo peroxidation in isolated chloroplasts. Archives of Biochemistry and Biophysics, 125, 850–857.CrossRefPubMedGoogle Scholar
  11. Hernandez, J. A., Jimenez, A., Mullineaux, P., & Sevilla, F. (2000). Tolerance of  pea (Pisum sativum) to long term salt stress is associated with induction of antioxidant defences. Plant Cell and Environment, 23, 853–862.Google Scholar
  12. Hernandez, J. A., Olmos, E., Corpas, F. J., Sevilla, F., & Del Rio, L. A. (1995). Salt induced oxidative stress in chloroplasts of pea plants. Plant Science, 105, 151–167.CrossRefGoogle Scholar
  13. Koca, H., Bor, M., Özdemir, F., & Türkan, İ. (2007). The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environmental and Experimental Botany, 60, 344–351.CrossRefGoogle Scholar
  14. Kochert, G. (1978). Carbohydrate determination by phenol-sulfuric acid method. In J. A. Hellebust & J. S. Craige (Eds.), Handbook of physiological methods: physiological and biochemical methods (pp. 95–97). London: Cambridge University Press.Google Scholar
  15. Lee, D. H., Kim, Y. S., & Lee, C. B. (2001). The inductive responses of the antioxidant enzymes by salt stress in the rice (Oryza sativa L.). Journal of Plant Physiology, 158, 737–745.CrossRefGoogle Scholar
  16. Liang, C., Feng, R., Hui, Z., Weimin, J., & Xuebao, L. (2010). Identification and expression analysis of genes in response to high-salinity and drought stresses in Brassica napus L. Acta Biochimica et Biophysica Sinica, 42, 154–164.CrossRefGoogle Scholar
  17. Lopez, F., Vansuyt, G., Casse-Delbart, F., & Fourcroy, P. (1996). Ascorbate peroxidase activity, not the mRNA level, is enhanced in salt-stressed Raphanus sativus plants. Physiologia Plantarum, 97, 13–20.CrossRefGoogle Scholar
  18. Lowry, O. H., Rosenbrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.PubMedGoogle Scholar
  19. MAPK Group. (2002). Mitogen-activated protein kinase cascades in plants: A new nomenclature. Trends in Plant Science, 7, 301–308.CrossRefGoogle Scholar
  20. Masood, A., Shah, N. A., Zeeshan, M., & Abraham, G. (2006). Differential response of antioxidant enzymes to salinity stress in two varieties of Azolla (Azolla pinnata and Azolla filiculoides). Environmental and Experimental Botany, 58, 216–222.CrossRefGoogle Scholar
  21. Meloni, D. A., Oliva, M. A., Martinez, C. A., & Cambraia, J. (2003). Photosynthesis and activity of superoxide dismutase peroxidase and glutathione reductase in cotton under salt stress. Environmental and Experimental Botany, 49, 69–76.CrossRefGoogle Scholar
  22. Meneguzzo, S., Navari-Izzo, F., & Izzo, R. (1999). Antioxidative responses of shoots and roots of wheat to increasing NaCl concentrations. Journal of Plant Physiology, 155, 27–280.CrossRefGoogle Scholar
  23. Misra, N., & Dwivedi, U. N. (2004). Genotypic difference in salinity tolerance of green gram cultivars. Plant Science, 166, 1135–1142.CrossRefGoogle Scholar
  24. Munns, R., & Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651–681.CrossRefPubMedGoogle Scholar
  25. Murashige, T., & Skoog, F. A. (1962). Revised medium for rapid growth and bioassays with tobacco cultures. Physiologia Plantarum, 159, 473–479.CrossRefGoogle Scholar
  26. Nakano, Y., & Asada, K. (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22, 867–880.Google Scholar
  27. Parida, A., Das, A. B., & Das, P. (2002). NaCl stress causes changes in photosynthetic pigments, proteins and other metabolic components in the leaves of a true mangrove, Bruguiera parviflora, in hydroponic cultures. Journal of Plant Biology, 45, 28–36.CrossRefGoogle Scholar
  28. Pedley, K. F., & Martin, G. B. (2005). Role of mitogen-activated protein kinases in plant immunity. Current Opinion in Plant Biology, 8, 541–547.CrossRefPubMedGoogle Scholar
  29. Rodriguez, M. C., Petersen, M., & Mundy, J. (2010). Mitogen-activated protein kinase signaling in plants. Annual Review of Plant Biology, 61, 621–649.CrossRefPubMedGoogle Scholar
  30. Sakamoto, A., & Murata, N. (2002). The role of glycine betaine in the protection of plants from stress: Clues from transgenic plants. Plant Cell and Environment, 25, 163–171.CrossRefGoogle Scholar
  31. Shirani Rad, A. H., Naeemi, M., & Nasr Esfahani, S. H. (2010). Evaluation of terminal drought stress tolerance in spring and winter rapeseed genotypes. Iranian Journal of Crop Sciences, 12, 112–126. (in Persian).Google Scholar
  32. Singh, S. K., Sharma, H. C., Goswami, A. M., Datta, S. P., & Singh, S. P. (2000). In vitro growth and leaf composition of grapevine cultivar as affected by sodium chloride. Biologia Plantarum, 43, 283–286.CrossRefGoogle Scholar
  33. Smirnoff, N. (1995). Antioxidant system and plant responses to the environment. In N. Smirnoff (Ed.), Environment and plant metabolism: Flexibility and acclimation (pp. 217–243). Oxford: Bios Scientific Publishers.Google Scholar
  34. Sreenivasulu, N., Grimm, R., Wobus, U., & Weachke, W. (2000). Differential response of antioxidant compounds to salinity stress in salt tolerant and salt sensitive seedlings of foxtail millet (Setaria italica). Physiologia Plantarum, 109, 435–442.CrossRefGoogle Scholar
  35. Stone, J. M., & Walker, J. C. (1995). Plant protein kinase families and signal transduction. Plant Physiology, 108, 451–457.CrossRefPubMedPubMedCentralGoogle Scholar
  36. Tijen, D., & Ismail, T. (2005). Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environmental and Experimental Botany, 53, 247–257.CrossRefGoogle Scholar
  37. Wang, Z., Mao, H., Dong, C., Ji, R., Cai, L., Fu, H., et al. (2009). Overexpression of Brassica napus MPK4 enhances resistance to Sclerotinia sclerotiorum in oilseed rape. Molecular Plant Microbe Interactions, 22, 235–244.CrossRefPubMedGoogle Scholar
  38. Xiong, L., & Yang, Y. (2003). Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. Plant Cell, 15, 745–759.CrossRefPubMedPubMedCentralGoogle Scholar
  39. Xiong, L., & Zhu, J. K. (2002). Molecular and genetic aspects of plant responses to osmotic stress. Plant Cell and Environment, 25, 131–139.CrossRefGoogle Scholar
  40. Yan, J. Y., Wang, J., Tissue, D., Holaday, A. S., Allen, R., & Zhang, H. (2003). Photosynthesis and seed production under water-deficit condition in transgenic tobacco plants that overexpress Arabidopsis ascorbate peroxidase gene. Crop Science, 43, 1477–1483.CrossRefGoogle Scholar
  41. Yang, Q., Chen, Z. Z., Zhou, X. F., Yin, H. B., Li, X., Xin, X. F., et al. (2009). Overexpression of SOS (salt overly sensitive) genes increase salt tolerance in transgenic Arabidopsis. Molecular Plant, 2, 22–31.CrossRefPubMedGoogle Scholar
  42. Yu, S., Zhang, L., Zuo, K., Tang, D., & Tang, K. (2005). Isolation and characterization of an oilseed rape MAP kinase BnMPK3 involved in diverse environmental stresses. Plant Science, 169, 413–421.CrossRefGoogle Scholar
  43. Zhang, X., Lu, G., Long, W., Zou, X., Li, F., & Nishio, T. (2014). Recent progress in drought and salt tolerance studies in Brassica crops. Breeding Science, 64, 60–73.CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Indian Society for Plant Physiology 2017

Authors and Affiliations

  • Fatemeh Rahmani
    • 1
  • Arghavan Peymani
    • 1
  • Abdollah Hassanzadeh Gorttapeh
    • 2
  1. 1.Biology Department, Faculty of SciencesUrmia UniversityUrmiaIran
  2. 2.Agricultural and Natural Resources Research Center of West AzarbijanUrmiaIran

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