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Study of genetic diversity of sugarcane (Saccharum) species and commercial varieties through TRAP molecular markers

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Abstract

Genetic variations were evaluated among the twenty-five sugarcane genotypes employing functional molecular (TRAP) markers. Genetic diversity exists among sugarcane germplasm was exploited to identify promising genotypes bearing enviable agronomic traits (sucrose content and multiple disease resistance). Genetically diversified genotype could be exploited as proven parents in sugarcane hybridization programs to establish a promising cross. TRAP markers amplify functional regions of the genome, a valuable information in relation to the variations within coding/functional regions of the plant genome. Genetic similarity (GS) among all the twenty-five genotypes was calculated in a wide range as 41–99% with an average of 70%. Thirteen unique DNA bands of Saccharum spontaneum and thirteen specific DNA bands for cultivar were produced by the used markers. Poly component analysis showed that 52.48% of the cumulative variation was explained by the all the genotypes with respect to the sucrose metabolism related genes. Sugarcane genotypes viz; CoS 96269 and CoS 8436 (GS 97.0%) and BO 91 and CoSe 95422 (GS 96%) showed highest genetic similarity. These genotypes may be recommended as proven parents for making enviable crosses in sugarcane breeding programs. Moreover, functional TRAP markers would be efficiently useful in genetic studies for sugarcane genetic improvement.

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Acknowledgements

Authors are thankful to Ministry of Sugar and Cane Development, Govt. of Uttar Pradesh, India, and Director, Sugarcane Research Institute (U.P. Council of Sugarcane Research) to providing financial aid for conducting the research work.

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Author notes

  1. R. B. Singh

    Present address: Quality and Basic Sciences Lab, ICAR-Indian Institute of Wheat and Barley Research (IIWBR), Post Box No. 158, Agrasain Marg, Karnal, 132 001, Haryana, India

Authors and Affiliations

  1. Department of Biotechnology, Punjab Technical University (PTU), Kapurthala, Punjab, 144 603, India

    R. B. Singh

  2. Swami Satyanand College of Management and Technology, Amritsar, Punjab, 143 001, India

    Balwant Singh

  3. Sugarcane Research Institute, (U.P. Council of Sugarcane Research), Shahjahanpur, Uttar Pradesh, 242 001, India

    R. B. Singh & R. K. Singh

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  1. R. B. Singh
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Correspondence to R. B. Singh.

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Singh, R.B., Singh, B. & Singh, R.K. Study of genetic diversity of sugarcane (Saccharum) species and commercial varieties through TRAP molecular markers. Ind J Plant Physiol. 22, 332–338 (2017). https://doi.org/10.1007/s40502-017-0314-z

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