Abstract
The study was carried out in order to evaluate the floral biology in terms of anthesis, anther dehiscence and in vitro stigma receptivity as well as pre and post-pollination biochemical changes in stigma of mango cvs. Alphonso, Totapuri and Amrapali, differing in fruit set intensity. Maximum anthesis was observed before 8:00 AM and anther dehiscence between 11:00 AM and 12:00 PM in all the cultivars. As the temperature increased, anther dehiscence advanced and coincided with higher anthesis and resulted in increased fruit set. Maximum stigma receptivity was observed within 3 h of anthesis in all the cultivars. The pH of stigmatic surface was neutral at the time of anthesis, anther dehiscence and at maximum stigma receptivity and changed to acidic in all the cultivars. Stigmatic surface indicated consistent increase in free sugars (Sucrose, Galactose, Glucose and Fructose), free amino acids (Serine, Citrulline, Alanine, Proline, Phenylalanine and Glutamic acid) and hormones (Abscisic acid, Salicylic acid and Jasmonic acid) after anthesis. Among the cultivars, Amrapali was found to be good in terms of floral biological observations as well as in stigma biochemical parameters resulting in higher fruit set.
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References
Ashok Kumar, M., & Reddy, Y. N. (2007). Effect of calcium nitrate and salicylic acid spray at flowering on transduction of flowering in cv. Baneshan. Crop Research, 34, 146–149.
Dafni, A. (1992). Pollination ecology: A practical approach. Oxford: Oxford University Press.
De Wet, E., & Robbertse, P. J. (1986). Pollen studies and stigma receptivity of Mangifera indica L. cvs Haden and Sensation. South African Mango Growers’ Association Yearbook, 6, 27–32.
Dupuis, I., & Dumas, C. (1990). Biochemical markers of female receptivity in maize (Zea mays L.) assessed using in vitro fertilization. Plant Science, 70, 11–20.
Gillaspy, G., Ben-David, H., & Gruissem, W. (1993). Fruits: a developmental perspective. The Plant Cell, 5, 1439–1451.
Gunjate, R. T., Jorwekar, D. P., & Lad, B. L. (1983). Pollination, fruit set and fruit drop in Alphonso mango. Journal of Maharashtra Agricultural University, 8, 168–170.
Herrero, M. (2003). Male and female synchrony and the regulation of mating in flowering plants. Philosophical Transactions of the Royal Society of London Series B Biological Sciences, 358, 1019–1024.
Indu Bala, J. (1990). Floral biology of three mango clones in relation to their pollination efficiency. MARDI. Journal of Tropical Agriculture and Food Science, 18, 9–17.
Jain, M., Prasad, P. V. V., Boote, K. J., Hartwell, A. L., & Chourey, P. S. (2007). Effects of season-long high temperature growth conditions on sugar-to-starch metabolism in developing microspores of grain sorghum (Sorghum bicolor L. Moench). Planta, 227, 67–79.
Kandasamy, M. K., & Vivekanandan, M. (1986). Stigmatic exudate: Its origin, physical nature, biochemical composition and function. Indian Review of Life Sciences, 6, 1–17.
Knox, R. B. (1984). Pollen–pistil interactions. In H. F. Linskens & J. Heslop-Harrison (Eds.), Cellular interactions (pp. 508–608). New York: Springer.
McInnis, S. M., Desikan, R., Hancock, J. T., & Hiscock, S. J. (2006). Production of reactive oxygen species and reactive nitrogen species by angiosperm stigmas and pollen: Potential signalling crosstalk? New Phytologist, 172, 221–228.
Nimbalkar, M. S., Pai, S. R., Pawar, N. V., Oulkar, D., & Dixit, G. B. (2012). Free amino acid profiling in grain Amaranth using LC-MS/MS. Food Chemistry, 134, 2565–2569.
Pan, X., Welti, R., & Wang, X. (2008). Simultaneous quantification of major phytohormone and related compounds in crude plant extracts by liquid chromatography–electrospray tandem mass spectrometry. Phyto-chemistry, 69, 1773–1781.
Pimentel, R. B., Coronel, R. E., & Espino, R. F. C. (1984). Floral biology and fruit set in mango (Mangifera indica L.) cultivars: ‘Carabao’, ‘Pico’ and ‘KanchaMitha’. Philippine Journal of Crop Science, 9, 47–51.
Prasad, P. V. V., Boote, K. J., Allen, Hartwell, & Jr, L. (2006). Adverse high temperature on pollen viability, seed-set, seed yield and harvest index of grain-sorghum [Sorghum bicolor (L.) Moench] are more severe at elevated carbon dioxide due to higher tissue temperature. Agricultural and Forest Meteorology, 139, 237–251.
Pusey, P. L., David, R. R., Eric, A. C., & Mattheis, J. P. (2008). Characterization of stigma exudates in aqueous extracts from apple and pear flowers. Horticulture Science, 43, 1471–1478.
Randhawa, G. S., & Damodaran, V. K. (1961). Studies on floral biology and sex ratio in mango (Mangifera indica L.) I. A review. Indian Journal of Horticulture, 18, 9–35.
Selvaraj, Y., & Kumar, R. (1994). Enzymatic regulation in ripening mango fruit. Indian Journal of Horticulture, 51, 316–323.
Seymour, R., & Blaylock, A. J. (2000). Stigma peroxidase activity in association with thermogenesis in Nelumbonucifera. Aquatic Botany, 67, 155–159.
Shi-Jin, O., Jian-hua, Z., Hong-Xiang, P., Tai-Ming, H., Quan-Guang, H., Xu-Ning, et al. (2007). Relationship between humidity, temperature and bisexual flower ratio of Kate mango. Chinese Journal of Ecology and Agriculture, 5, 21–25.
Shivanna, K. R. (2003). Pollen biology and biotechnology. New Delhi: Science Publishers Inc., Enfield/Plymouth.
Shu, Z. H. (1982). Scanning electron microscope observation on the floral ontogeny and development of mango (Mangifera indica L.). Journal of Agricultural Research of China, 31, 313–320.
Shu, Z. H. (1999). Effect of temperature on the flowering biology and fertilization of mangoes (Mangifera indica L.). Journal of Applied Horticulture, 1, 79–83.
Singh, L. B. (1960). The mango. Botany, cultivation and utilization (p. 439). London: Leonard Hill.
Singh, R. N. (1990). Mango. Indian council of agricultural research Book series No. 3 (Revised), New Delhi, p. 134.
Spencer, J. L., & Kennard, W. C. (1955). Studies on mango (Mangifera indica L.) fruit set in Puerto Rico. Tropical Agriculture Trin, 32, 323–330.
Steppuhn, A., & Wackers, F. L. (2004). HPLC sugar analysis reveals the nutritional state and the feeding history of parasitoids. Functional Ecology, 18, 812–819.
Stosser, R., Hartman, W., & Anvari, S. F. (1996). General aspects of pollination and fertilization of pome and stone fruits. Acta Horticulturae, 423, 15–21.
Stpiczynska, M. (2003). Stigma receptivity during the life span of Platantherachlorantha Custer (Rchb,) flowers. Acta Biologica Cracoviensia, Series Botanica, 45, 37–41.
Subhas Chander, M. (1990). Enzymatic associations with resistance to rust and powdery mildew in peas. Indian Journal of Horticulture, 47, 341–345.
Tsang, M. J., & Chang, W. N. (1983). Effects of temperature and growth regulators on flowering and fruit set of mango. Journal of Chinese Society of Horticulture Sciences, 29, 87–95.
Usman, M., Fatima, B., & Jaskani, M. J. (2001). Review breeding in mango. International Journal of Agriculture and Biology, 3, 522–526.
Young, T. W. (1955). Influence of temperature on growth of mango pollen. Proceedings of the Florida state Horticultural Society, 68, 308–313.
Acknowledgements
This work was supported by grants from the Indian Council for Agricultural Research [ICAR] under the Project NICRA (National Innovation on Climate Resilient Agriculture). The authors are grateful to the Director, Indian Institute of Horticultural Research for providing the necessary facilities.
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Geetha, G.A., Shivashankara, K.S. & Roy, T.K. Floral biology and stigma biochemistry of mango cultivars with contrasting fruit set. Ind J Plant Physiol. 21, 537–544 (2016). https://doi.org/10.1007/s40502-016-0266-8
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DOI: https://doi.org/10.1007/s40502-016-0266-8