Abstract
Adipose-derived stem cells within subcutaneous adipose tissue were first reported in 2001. Since then, the number of regenerative medicine studies investigating adipose-derived stem cells (ASCs) has increased dramatically. This has included not only basic research in regenerative medicine using ASCs but also has progressed and clinical safety studies have now commenced in Japan. Translational research (including clinical research and clinical trials) using cell therapy aims to verify the possibility of achieving repair of tissue or its function and investigating the safety of these protocols. However, despite this simplicity, elucidating the mechanism of action of implanted processed cells is difficult. This indicates the necessity for the further discussion focusing on translational research for cell therapy, as well as continuing to develop novel therapeutic strategies. In this paper, we discuss the feasibility of practical application of using ASCs for periodontal tissue regeneration therapy and discuss the legal system for promoting the practical application of regenerative medicine recently enforced in Japan.
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References
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Zuk PA et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7(2):211–28. This manuscript is the first study of adipose-derived stem cells, and presents the possibility of stem cell therapy with adipose tissue.
Caplan A. Mesenchymal stem cells. J Orthop Res. 1991;9(5):641–50.
Díez-Tejedor E et al. Reparative therapy for acute ischemic stroke with allogeneic mesenchymal stem cells from adipose tissue: a safety assessment: a phase II randomized, double-blind, placebo-controlled, single-center, pilot clinical trial. J Stroke Cerebrovasc Dis. 2014;23(10):2694–700.
Garcia-Olmo D et al. Expanded adipose-derived stem cells for the treatment of complex perianal fistula: a phase II clinical trial. Dis Colon Rectum. 2009;52(1):79–86.
Esposito M et al. Enamel matrix derivative (Emdogain) for periodontal tissue regeneration in intrabony defects. A Cochrane systematic review. Eur J Oral Implantol. 2009;2(4):247–66.
Nevins M et al. Platelet-derived growth factor stimulates bone fill and rate of attachment level gain: results of a large multicenter randomized controlled trial. J Periodontol. 2005;76(12):2205–15.
Singh P, Suresh D. Clinical evaluation of GEM 21S(®) and a collagen membrane with a coronally advanced flap as a root coverage procedure in the treatment of gingival recession defects: a comparative study. J Indian Soc Periodontol. 2012;16(4):577–83.
Polimeni G, Xiropaidis AV, Wikesjö UM. Biology and principles of periodontal wound healing/regeneration. Periodontol 2000. 2006;41:30–47.
Tobita M, Mizuno H. Periodontal disease and periodontal tissue regeneration. Curr Stem Cell Res Ther. 2010;5(2):168–74.
Iwata T et al. Periodontal regeneration with multi-layered periodontal ligament-derived cell sheets in a canine model. Biomaterials. 2009;30(14):2716–23.
Tobita M et al. Periodontal tissue regeneration with adipose-derived stem cells. Tissue Eng A. 2008;14(6):945–53. This manuscript is the first study of periodontal tissue regeneration with adipose-derived stem cells.
Tobita M et al. Periodontal tissue regeneration by combined implantation of adipose tissue-derived stem cells and platelet-rich plasma in a canine model. Cytotherapy. 2013;15(12):1517–26.
Zuk PA et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002;13(12):4279–95.
Yoshimura K et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol. 2006;208(1):64–76.
Mitchell JB et al. Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers. Stem Cells. 2006;24(2):376–85.
Wang Y et al. Bone marrow-derived cells homing for self-repair of periodontal tissues: a histological characterization and expression analysis. Int J Clin Exp Pathol. 2015;8(10):12379–89.
Tsumanuma Y et al. Allogeneic transplantation of periodontal ligament-derived multipotent mesenchymal stromal cell sheets in canine critical-size supra-alveolar periodontal defect model. Biores Open Access. 2016;5(1):22–36.
Jo C et al. Intra-articular injection of mesenchymal stem cells for the treatment of osteoarthritis of the knee: a proof-of-concept clinical trial. Stem Cells. 2014;32(5):1254–66.
Houtgraaf JH et al. First experience in humans using adipose tissue-derived regenerative cells in the treatment of patients with ST-segment elevation myocardial infarction. J Am Coll Cardiol. 2012;59(5):539–40.
Tzouvelekis A et al. A prospective, non-randomized, no placebo-controlled, phase Ib clinical trial to study the safety of the adipose derived stromal cells-stromal vascular fraction in idiopathic pulmonary fibrosis. J Transl Med. 2013;11:171.
Tobita M, Mizuno H. Adipose-derived stem cells and platelet-rich plasma: the keys to functional periodontal tissue engineering. Curr Stem Cell Res Ther. 2013;8(5):400–6.
Sawada K et al. Trophic factors from adipose tissue-derived multi-lineage progenitor cells promote cytodifferentiation of periodontal ligament cells. Biochem Biophys Res Commun. 2015;464(1):299–305.
Choi SA et al. Preclinical biosafety evaluation of genetically modified human adipose tissue-derived mesenchymal stem cells for clinical applications to brainstem glioma. Stem Cells Dev. 2016;25(12):897–908.
Blum B, Benvenisty N. The tumorigenicity of human embryonic stem cells. Adv Cancer Res. 2008;100:133–58.
Knoepfler PS. Deconstructing stem cell tumorigenicity: a roadmap to safe regenerative medicine. Stem Cells. 2009;27(5):1050–6.
Jung JW et al. Familial occurrence of pulmonary embolism after intravenous, adipose tissue-derived stem cell therapy. Yonsei Med J. 2013;54(5):1293–6.
Kilroy GE et al. Cytokine profile of human adipose-derived stem cells: expression of angiogenic, hematopoietic, and pro-inflammatory factors. J Cell Physiol. 2007;212:702–9.
Suga H et al. Paracrine mechanism of angiogenesis in adipose-derived stem cell transplantation. Ann Plast Surg. 2014;72(2):234–41.
Pallua N, Serin M, Wolter TP. Characterisation of angiogenetic growth factor production in adipose tissue-derived mesenchymal cells. J Plast Surg Hand Surg. 2014;48(6):412–6.
Josh F et al. Concentration of PDGF-AB, BB and TGF-beta 1 as valuable human serum parameters in adipose-derived stem cell proliferation. J Nippon Med Sch. 2013;80(2):140–7.
Josh F et al. Concentration of PDGF-AB, BB and TGF-β1 as valuable human serum parameters in adipose-derived stem cell proliferation. J Nippon Med Sch. 2013;80(2):151–2.
Gehmert S et al. Angiogenesis: the role of PDGF-BB on adipose-tissue derived stem cells (ASCs). Clin Hemorheol Microcirc. 2011;48(1):5–13.
Rodriguez TM et al. Effect of TGF-beta1 stimulation on the secretome of human adipose-derived mesenchymal stromal cells. Stem Cells Transl Med. 2015;4(8):894–8.
Bagno LL et al. Sustained IGF-1 secretion by adipose-derived stem cell improves infarcted heart function. Cell Transplant. 2015;25(9):1609–22.
Song SY, Chung HM, Sung JH. The pivotal role of VEGF in adipose-derived-stem-cell-mediated regeneration. Expert Opin Biol Ther. 2010;10(11):1529–37.
Taylor-Weiner H, Graff Zivin J. Medicine’s wild west—unlicensed stem-cell clinics in the United States. N Engl J Med. 2015;373(11):985–7. This manuscript presents concern of cell therapies in the private practice.
Tobita M et al. Japan’s challenges of translational regenerative medicine: act on the safety of regenerative medicine. Regen Ther. 2016;4:78–81. This manuscript explains the act on the safety of regenerative medicine in Japan.
Konomi K et al. New Japanese initiatives on stem cell therapies. Cell Stem Cell. 2015;16(4):350–2.
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Morikuni Tobita declares that he has no conflict of interest.
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This article is part of the Topical Collection on Orodental Regenerative Medicine
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Tobita, M. Feasibility of Periodontal Tissue Regeneration Therapy with Adipose-Derived Stem Cells. Curr Oral Health Rep 3, 314–318 (2016). https://doi.org/10.1007/s40496-016-0113-z
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DOI: https://doi.org/10.1007/s40496-016-0113-z