Skip to main content
Log in

Unlocking the antioxidant and antimicrobial potential of flavone and amide-rich fractions from Conchocarpus macrocarpus (Rutaceae) leaves

  • Biochemistry & Physiology - Original Article
  • Published:
Brazilian Journal of Botany Aims and scope Submit manuscript

Abstract

Conchocarpus macrocarpus (Rutaceae) is an endemic species in Brazil, whose chemical and biological properties are poorly understood. This study aimed to evaluate the antioxidant and antimicrobial potential of the butanolic partition phase extracted from C. macrocarpus leaves collected in Espírito Santo, Brazil. In vitro antioxidant assays, including DPPH radical scavenging and Folin–Ciocalteu assay, and cytotoxicity and antioxidant activity in RAW cells using the MTT method were performed. Additionally, antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Candida albicans was investigated. Results showed that the flavone-rich fraction exhibited the strongest antioxidant activity, as observed for the PF-BuOH G2.1 fraction that showed values of 237.2 ± 1.4 mg TE/g of extract and 118.2 ± 1.6 mg GAE/g of extract. However, the samples did not show protective effects against hydrogen peroxide. Furthermore, the flavones and amides present in the PF-BuOH G2.1 fraction displayed antimicrobial activity against C. albicans, showing a minimum inhibitory concentration of 1.25 mg/mL at the highest microbial load (1.5 × 105 CFU/mL). Our findings provide insights into the chemical composition and biological potential of C. macrocarpus and highlight the promising applications of the BuOH partition phase and its fractions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Data availability

All data are included in this article (and its supplementary information files).

References

Download references

Acknowledgements

The authors would like to express their gratitude to the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (Process number: 88887.699686/2022-00) for providing the PhD research grants to ERS and to CNPq (Process number: 304699/2018-7) for awarding the scholarship to IBS. Additionally, the authors would like to acknowledge Aline Bertinatto Cruz and Mourisa Ferreira for their valuable technical assistance.

Author information

Authors and Affiliations

Authors

Contributions

ERS and DYACS conceptualized and designed the study, critically revised the manuscript, and actively contributed to data discussions. PBT and ERS conducted the in vitro antioxidant activity, and actively participated in data discussions. ERS prepared the extracts, performed statistical analyses, conducted the experiments, and identified the compounds. KCS and HAOR performed the in vivo antioxidant experiments. IBS and JSS assessed the antimicrobial activity. All authors critically reviewed and approved the final version of the manuscript.

Corresponding authors

Correspondence to Elielson Rodrigo Silveira or Déborah Yara Alves Cursino dos Santos.

Ethics declarations

Conflict of interest

The authors declare that they have no conflicts of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 26 kb)

Supplementary file2 (DOCX 15 kb)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Silveira, E.R., Torres, P.B., Scortecci, K.C. et al. Unlocking the antioxidant and antimicrobial potential of flavone and amide-rich fractions from Conchocarpus macrocarpus (Rutaceae) leaves. Braz. J. Bot 46, 853–866 (2023). https://doi.org/10.1007/s40415-023-00927-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40415-023-00927-3

Keywords

Navigation