Abstract
Medicinal plants have been used for the treatment of diseases and its demand is expanding in both developed and developing countries. The extensive consumption to meet the demand and supply ratio exerts a heavy strain on the existing resources. This subsequently led to adulteration and substitution. The DNA-based method is the most widely adopted for the determination of adulteration in herbal products. Quality DNA extraction is regarded as the prerequisite for the application of DNA-based techniques in herbal plant authentication. Various protocols for DNA isolation could only be applied to a small number of species and are often expensive and time-consuming. Furthermore, herbal products undergo various conditions and are mixed with different substances, these factors affect the integrity of the DNA making it difficult to isolate. In this study, a simple, cost-effective and rapid CTAB-based DNA extraction protocol was developed which successfully isolated quality DNA from different samples of medicinal plants and their respective adulterants without using liquid nitrogen. Quality DNA was also isolated from different herbal products of reputed companies. The protocol was also validated by the isolation of quality DNA from different herbarium samples. DNA quality of both plants and herbal drugs was measured by A260/A280 ratio, which was ranging between 1.3 and 1.8. Thus, the developed protocol can be adapted for medicinal plants and herbal drugs quality DNA isolation and authentication as well as herbarium samples for molecular identifications. The isolated DNA was found to be of good quality and sufficient quantity for ISSR-PCR, SCoT-PCR and restriction digestion reactions. The protocol has successfully demonstrated its suitability for different genomics downstream applications.
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Acknowledgements
The authors express their gratitude to the management of Marwadi University, Rajkot, Gujarat, for providing the necessary facilities for conducting the research work, and equally grateful to Dr. Gaurav Sanghvi, (Head of the Department, Microbiology) for his encouragement in carrying out the investigation.
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MI carried out the investigation, sample collection, methodology, data curation and analysis, writing of original draft; AD contributed to methodology, data curation and analysis; AKB conceptualization of the investigation, supervision, analyzed the results, correction and editing of the final draft.
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Ibrahim, M., Detroja, A. & Bishoyi, A.K. A rapid, efficient and cost-effective DNA isolation protocol for various herbal products which is appropriate for different downstream genomics applications. Braz. J. Bot 46, 873–880 (2023). https://doi.org/10.1007/s40415-023-00923-7
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DOI: https://doi.org/10.1007/s40415-023-00923-7