Abstract
Chresta is a genus mostly endemic to Brazil that presents several rupicolous species with naturally fragmented distributions. Aiming to facilitate studies about genetic diversity and structure in these species, we developed a set of 22 nuclear and 6 plastid microsatellite markers that are transferable among different species of the genus. We used previously obtained genomic data from target capture and Illumina sequencing to identify putative repeat regions, designed and synthesized primers, and genotyped individuals from different populations of three species. All loci were successfully amplified in all three species and were overall variable, except for the plastid markers, which were monomorphic in two species. These newly developed microsatellites will be useful in studies focusing on the population genetics of Chresta.
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Acknowledgements
This research was supported by two FAPESP (Fundação de Amparo a Ciência do Estado de São Paulo) scholarships (2013/18189-2, 2016/12446-1) and the National Science Foundation Division of Environmental Biology DEB-1745197. We would like to thank Tom Cunningham from the MRC at UT-Memphis for carrying out the genotyping, Gabbie Johson for helping with DNA extractions and Ramhari Thapa for the useful discussions and advice.
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CMS designed the study, sampled specimens in the field, did laboratory work, analyzed the data and wrote the manuscript. BL helped with field collections, provided data about the occurrence of each species and reviewed the manuscript. JRP reviewed and commented in several versions of the manuscript. JRM contributed to the study design and laboratory routines, provided reagents and equipment for genotyping, helped in data analysis and reviewed the manuscript.
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Siniscalchi, C.M., Loeuille, B., Pirani, J.R. et al. Using genomic data to develop SSR markers for species of Chresta (Vernonieae, Asteraceae) from the Caatinga. Braz. J. Bot 42, 661–669 (2019). https://doi.org/10.1007/s40415-019-00569-4
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DOI: https://doi.org/10.1007/s40415-019-00569-4