Viability of biodiesel production from a thermophilic microalga in conventional and alternative culture media
Microalgae biodiesel production depends on several factors to minimize the costs of the production process from both biomass and biodiesel. In some outdoor systems, the temperature can be higher than 35 °C, which is lethal for several algae. Prospecting microalga from thermal environments seems to be a good option. Therefore, the objective of this work was to isolate a microalga (Acutodesmus obliquus (Turpin) Hegewald and Hanagata) from thermal water and evaluate its cultivation productivity in Bold Basal Medium (BBM) and in lower cost alternative media. One alternative medium contained only the main growth ingredients (DAF), the other included these same ingredients with the addition of wastewater from the purification of grease-based raw materials (DAF + OGR). Microalga biodiesel productivity was also compared with the biodiesel yield of soybean, which is one of the main raw materials currently used for biodiesel production. The microalga was shown to provide biomass with similar productivity using the three different culture media in log phase. The microalga exhibited biodiesel productivity from 46 to 61 times higher than soybean; using 5.5–7.2% of the water and 1.6–2.2% of the land required for soybean cultivation to produce the same amount of biodiesel. The DAF + OGR medium, which costs 29% of the cost of the BBM medium, proved to be an efficient alternative medium compared to other in biomass productivity. Levels of tri-unsaturated and polyunsaturated fatty acids from A. obliquus microalga were slightly higher than those standardized by EN14214, requiring that the biodiesel be mixed with antioxidants.
KeywordsBiofuels Cost Fatty acids Wastewater
The infrastructure used in this project was funded by a project grant from the Financier of Studies and Projects (FINEP) and Foundation of Research Support (FUNAPE) of the Brazilian Ministry of Science, Technology, Innovation and Communications (MCTIC), Process No. 01.10.0457.00. National Council for Scientific and Technological Development also funded this project (CNPq), Process No. 407556/2013-3. NRAF is a CNPq fellow, Process No. 312019/2013-0. EBD is a CNPq fellow, Process No. 141501/2013-8. Alene Alder-Rangel reviewed the English language.
EBD performed the experiments, collected, and cultivated the microalga, analyzed the collected data, and wrote the main part of the manuscript. JP analyzed the chemical elements in ICP-OES. ATS analyzed the gas and mass chromatography data of fatty acids. NRAF conceived and designed the research, reviewed the manuscript, participated in writing, and obtained a grant from the FINEP & FUNAPE (Financiadora de Estudos e Projetos & Fundação de Apoio a Pesquisa) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico).
- Andersen RA (2005) Algal culturing techniques. Elsevier Academic Press, BurlingtonGoogle Scholar
- Apha (1998) Standard methods for examination of water and wastewater, 20th edn. Ediciones Diaz de Santos S.A/American Public Health Association; American Water Works Association/Water Pollution Control Federation, MadridGoogle Scholar
- Azeredo WA (2014) Otimização da produção de biodiesel metílico a partir de óleos de fritura residuais (OFR). Universidade Federal de GoiásGoogle Scholar
- Bajhaiya A, Kkmandotra S, Suseela MR (2010) Algal biodiesel: the next generation biofuel for India. Asian J Exp Biol Sci 1:728–739Google Scholar
- Barsanti L, Gualtieri P (2006) Algae: anatomy, biochemistry, and biotechnology. CRC Press Book, Boca RatonGoogle Scholar
- Brasil (2016) Lei no 13.263, de 23 de março de 2016. Diário Of da União 1:1Google Scholar
- Camargo RPL (2016) Produção e avaliação físico-química e ecotoxicológica de biodiesel etílico de óleos residuais de fritura. Universidade Federal de GoiásGoogle Scholar
- Campos JEG, de Almeida L (2012) Balanço térmico aplicado à recarga artificial dos aquíferos da região de Caldas Novas, estado de Goiás. Braz J Geol 42:196–207Google Scholar
- Carrim AJ (2016) Produção e avaliação físico-química, ecotoxicológica e microbiológica de biodiesel metílico de óleo residual de fritura (ORF). Universidade Federal de GoiásGoogle Scholar
- Chisti Y (2012) Raceways-based production of algal crude oil. In: Posten C, Walter C (eds) Microalgal biotechnology: potential and production. de Gruyter, Berlin, pp 113–146Google Scholar
- European Union (2010) EN 14214 Automotive fuels—fatty acid methyl esters (FAME) for diesel engines—Requirements and test methods. In: European Committee for StandardizationGoogle Scholar
- Franzese PP, Cavalett O, Häyhä T, D’Angelo S (2013) Integrated environmental assessment of agricultural and farming production systems in the Toledo River Basin (Brazil). United Nations Educational, ParisGoogle Scholar
- Lobato EJV (2005) Estação evaporimétrica de Goiânia: normais climatológicas (1975–2004). EAEA, GoiâniaGoogle Scholar
- Lourenço SO (2006) Cultivo de microalgas marinhas: princípios e aplicações. RimaGoogle Scholar
- Muylaert K, Beuckels A, Depraetere O et al (2015) Wastewater as a source of nutrients for microalgae biomass production. In: Moheimani NR, McHenry MP, de Boer K, Bahri PA (eds) Biomass and biofuels from microalgae. Springer, Cham, pp 75–94Google Scholar
- Onay M, Sonmez C, Oktem HA, Yucel AM (2014) Thermo-resistant green microalgae for effective biodiesel production: isolation and characterization of unialgal species from geothermal flora of Central Anatolia. Bioresour Technol 169:62–71. https://doi.org/10.1016/j.biortech.2014.06.078 CrossRefPubMedGoogle Scholar