Brazilian Journal of Botany

, Volume 41, Issue 1, pp 11–19 | Cite as

Effects of apple fruit fermentation (AFF) solution on growth and fruit quality of apple trees

  • Jie Zhang
  • Hui Pang
  • Ji Tian
  • Mingjuan Liu
  • Qianlong Ji
  • Yuncong Yao
Original Article


It has been demonstrated that the use of organic materials in fruit orchards can effectively increase soil fertility, fruit production and fruit quality. Insect and disease damage, crop thinning, natural drop cause abscission and abscised parts are a source of organic additives for orchard production. In this study, we developed a fermented nutrient solution composed of young apple fruits, brown sugar, a fermenting agent and water. Diluted solutions (1:100 and 1:200) of this nutrient solution were sprayed on 2-year-old and 12-year-old apple (Malus domestica (Borkh.) ‘Fuji’) trees. The results showed that foliar spraying of the fermentation solutions significantly improved tree height, trunk circumference, shoot growth and leaf area of young apple trees, and increased the fruit weight, shape index, firmness, soluble solid content, volatile compound content and vitamin C content of adult apple trees, as compared to the control treatment. The effects of the 1:200 diluted nutrient solution were significantly greater than those of the 1:100 diluted nutrient solution. Foliar spray of a nutrient solution derived from young apple fruit promoted vegetative growth and fruit quality.


Fruit production and quality Nutrient solution Soil fertility 



We would like to thank the Beijing Collaborative Innovation Centre for Eco-Environmental Improvement with Forestry and Fruit Trees, the Key Laboratory of Pomology at the Beijing University of Agriculture, the Beijing Nursery Engineering Research Center for Fruit Crops, the Key Laboratory of Agricultural Applications at the Beijing University of Agriculture, as well as all of the technicians at the China–Japan Friendly Sightseeing Orchard in the Changping District and the Changping Forestry Administration in Beijing.


This work was supported by the National Key Research Program (2016YFD0201116), the Beijing Key Project of Science and Technology Plan (D161100000716003), the Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions and the Beijing Municipal Science and Technology Commission under Grant Number PXM2016001.

Supplementary material

40415_2017_418_MOESM1_ESM.docx (18 kb)
Supplementary material 1 (DOCX 18 kb)


  1. Busatto N, Farneti B, Tadiello A, Vrhovsek U, Cappellin L, Biasioli F, Velasco R, Costa G, Costa F (2014) Target metabolite and gene transcription profiling during the development of superficial scald in apple (Malus × domestica Borkh). BMC Plant Biol 14:1–13CrossRefGoogle Scholar
  2. Chen YH, Liu L, Guo QS, Zhu Z, Zhang L (2016) Effects of different water management options and fertilizer supply on photosynthesis, fluorescence parameters and water use efficiency of Prunella vulgaris seedlings. Biol Res 49:12CrossRefPubMedCentralPubMedGoogle Scholar
  3. Ciganda V, Gitelson A, Schepers J (2009) Non-destructive determination of maize leaf and canopy chlorophyll content. J Plant Physiol 166:157–167CrossRefPubMedGoogle Scholar
  4. Dai ML, Li ST, Han ZH, Xu XF, Zhang XZ, Li TZ (2008) Effects of plant derived nutrient solutions on the growth and fruit quality of ‘Xueqing’ pear. J China Agric Univ 13:19–23 (in Chinese) Google Scholar
  5. Elsin RD, Sai KM, Srilatha N, Rajagopal S (2016) Iron deficiency cause changes in photochemistry, thylakoid organization, and accumulation of photosystem II proteins in Chlamydomonas reinhardtii. Photosynth Res 130:469–478CrossRefGoogle Scholar
  6. Feng FJ, Li MJ, Ma FW, Cheng LL (2014) Effects of location within the tree canopy on carbohydrates, organic acids, amino acids and phenolic compounds in the fruit peel and flesh from three apple (Malus × domestica) cultivars. Hortic Res 1:14019CrossRefPubMedCentralPubMedGoogle Scholar
  7. Geng J, Cui NN, Zhang J, Zhou ZQ, Yao YC (2011) Effect of aromatic plant-derived nutrient solution on the growth, fruit quality and disease prevention of pear trees. Acta Ecol Sin 31:1285–1294 (in Chinese) Google Scholar
  8. Han NR (2006) 21st century organic agriculture. China Agricultural University Press, Beijing, pp 183–189Google Scholar
  9. Jakopic J, Veberic R, Zupancic K, Štampar F (2007) Influence of nitrogen on the contents of carbohydrates and organic acids in apples (Malus domestica Borkh.) cv. ‘golden delicious’. Eur J Hortic Sci 72:66–72Google Scholar
  10. Jirovetz L, Buchbauer G, Geissler M, Ngassoum MB, Parmentier M (2003) Pulp aroma compounds of untreated, boiled and roasted African pear [Dacryodes edulis (G. Don) H.J. Lam] fruits from cameroon by HS-SPME analysis coupled with GC/FID and GC/MS. Eur Food Res Technol 218:40–43CrossRefGoogle Scholar
  11. Liu YN, Cui NN, Zhang J, Yu FM, Zhang LB, Yao YC (2011) Effect of aromatic plant-derived nutrient solution on inhibition of harmful bacteria and nutrition for pear plants. Sci Agric Sin 44:3981–3990 (in Chinese) Google Scholar
  12. Liu BH, Cheng L, Li MJ, Liang D, Zou YJ, Ma FW (2012) Interactive effects of water and nitrogen supply on growth, biomass partitioning, and water-use efficiency of young apple trees. Afr J Agric Res 7:978–985Google Scholar
  13. Luo R, Zhang J, Liu MJ, Ji QL, Yao YC (2012) Method of making pear fruit-derived nutrient solution and its effect on photosynthetic characteristics and fruit quality. Sci Agric Sin 45:3337–3345 (in Chinese) Google Scholar
  14. Ribeiro NG, Roque DA (2006) Seasonality of nutrients in leaves and fruits of apple trees. Sci Agric 63:493–501CrossRefGoogle Scholar
  15. Sachdev DP, Cameotra SS (2013) Biosurfactants in agriculture. Appl Microbiol Biotech 97:1005–1016CrossRefGoogle Scholar
  16. Sara CC, Jose OF, Isabel MF (2002) HPLC/UV determination of organic acids in fruit juices and nectars. Eur Food Res Technol 214:67–71CrossRefGoogle Scholar
  17. Shi YW, Lou K, Li C (2010) Growth and photosynthetic efficiency promotion of sugar beet (Beta vulgaris L.) by endophytic bacteria. Photosynth Res 105:5–13CrossRefPubMedGoogle Scholar
  18. Siddiqui S, Bangerth F (1995) Effect of pre-harvest application of calcium on flesh firmness and cell-wall composition of apples-influence of fruit size. J Hortic Sci 70:263–270CrossRefGoogle Scholar
  19. Swietlik DF (1984) Foliar nutrition of fruit crops. Hortic Rev 6:287–355Google Scholar
  20. Tian SL, Sun XL, Shen GN, Xu L (2015) Effects of compound fertilizer of (NH2)2CO and KH2PO4 on the chestnut photosynthesis characteristics, growth and fruiting. J Appl Ecol 26:747–754Google Scholar
  21. Wang XD, Shi DC, Song Y, Zhai H (2005) GC-MS analysis of fruit aroma components of organic ‘Fuji’ apple. Acta Hortic Sin 17:2262–2264 (in Chinese) Google Scholar
  22. Wang RJ, Yao YC, Qi YP, Li XM, Ji QL (2012) Induction of early resistance response to Alternaira alternata. i f. sp. mali in apple and chitosan fermentation broth. Acta Ecol Sin 32:2239–2247 (in Chinese) CrossRefGoogle Scholar
  23. Zhang J, Shao W, Jiang R, Ji QL, Yao YC (2015) Study of the optimal production process and application of apple fruit [Malus domestica (L.) Borkh] fermentation. Pak J Bot 47:279–286Google Scholar
  24. Zhang J, Pang H, Ma MX, Bu YF, Shao W, Huang WJ, Ji QL, Yao YC (2016) An apple fruit fermentation (AFF) treatment improves the composition of the rhizosphere microbial community and growth of strawberry (Fragaria × ananassa Duch ‘Benihoppe’) seedlings. PLoS ONE 11:e0164776CrossRefPubMedCentralPubMedGoogle Scholar
  25. Zhu XG, Baker GNR, Eric S, Ort DR, Long SP (2005) Chlorophyll a fluorescence induction kinetics in leaves predicted from a model describing each discrete step of excitation energy and electron transfer associated with Photosystem II. Planta 223:114–133CrossRefPubMedGoogle Scholar

Copyright information

© Botanical Society of Sao Paulo 2017

Authors and Affiliations

  • Jie Zhang
    • 1
    • 2
  • Hui Pang
    • 1
    • 2
  • Ji Tian
    • 1
    • 2
  • Mingjuan Liu
    • 1
  • Qianlong Ji
    • 3
  • Yuncong Yao
    • 1
    • 2
  1. 1.College of Plant Science and TechnologyBeijing University of AgricultureBeijingChina
  2. 2.Beijing Key Laboratory for Agricultural Application and New TechniqueBeijing University of AgricultureBeijingChina
  3. 3.College of Biological Science and EngineeringBeijing University of AgricultureBeijingChina

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