Brazilian Journal of Botany

, Volume 40, Issue 2, pp 389–397 | Cite as

Comparative efficacy of BAU-Biofungicide and synthetic fungicides in management of diseases of rice (Oryza sativa L.) for quality seed production

Original Article


Extracts of garlic (Allium sativum L.) and neem (Azadirachta indica A. Juss.), BAU-Biofungicide (Trichoderma based preparation), Potent 250 EC (Propiconazole) and Bavistin DF (Carbendazim) were evaluated under laboratory and field conditions in controlling diseases of rice cv BR11 for quality seed production. BAU-Biofungicide (2%) showed significant effect in controlling mycelial growth of (Bipolaris oryzae (Breda de Haan) Shoem.), Cercospora oryzae I. Miyake, Rhizoctonia solani Kuhn. and Ustilaginoidea virens (Cke.) Tak. in-vitro test and also reduced disease severity of brown spot, narrow brown leaf spot, sheath blight and false smut as of Propiconazole (0.1%). Carbendazim (0.1%) showed better result in inhibiting the disease severity of narrow brown leaf spot and sheath blight. Propiconazole (0.1%) increased highest 26.40% grain yield, while BAU-Biofungicide (3%) increased 20.42% grain yield over untreated control in the field. Formation of maximum (74.33%) apparently healthy seed, highest germination (98.00%) increased 36.49% of vigor index by BAU-Biofungicide (2%) over control. Major seed borne fungi of harvested seeds were controlled by foliar application of BAU-Biofungicide.


Chemicals Plant extracts Propiconazole Trichoderma based preparation Yield 


  1. Agarwal PC, Mortensen CN, Mathur SB (1989) Seed-borne diseases and seed health testing of rice, chap 14. Technical Bulletin No. 3, Phytopathlogical Paper No. 30, CAB International Mycological Institute (CMI) Kew, Surrey, pp 58–59Google Scholar
  2. Ahmad S, Mukesh S (2002) Evaluation of fungal and bacterial antagonists against Fusarium nioniiiforme causing foot rot disease of rice. Ann Agric Res 23:319–321.Google Scholar
  3. Baki AA, Anderson JD (1973) Vigour determination of soybean seed by multiple criteria. Crop Sci 13:630–633CrossRefGoogle Scholar
  4. BBS (2014) Agriculture Wing, Bangladesh Bureau of Statistics. Ministry of Planning. Government of the People’s Republic of Bangladesh, Dhaka.Google Scholar
  5. Biswas SK, Ratan V, Srivastava SSL, Ramesh S (2008) Influence of seed treatment with biocides and foliar spray with fungicides for management of Brown leaf spot and Sheath blight of paddy. Indian Phytopath 61:55–59Google Scholar
  6. BRRI (1999) Some appropriate technologies of rice cultivation (Bengali Bulletin). Bangladesh Rice Research Institute, GazipurGoogle Scholar
  7. BRRI (2004) Modern rice cultivation. Bangladesh Rice Research Institute, GazipurGoogle Scholar
  8. Chen Y, Zhang Y, Yao J, Li YF, Yang X, Wang W-X, Zhang Gao AF (2013) Frequency distribution of sensitivity of Ustilaginoidea virens to four EBI fungicides, prochloraz, difenoconazole, propiconazole and tebuconazole, and their efficacy in controlling rice false smut in Anhui Province of China. Phytoparasitica 41:277–284CrossRefGoogle Scholar
  9. Chowdhury MMH (2012) Evaluation of quality status and management Truthfully Labeled Seed (TLS) of rice in Bangladesh, Ph.D. Thesis, Bangladesh Agricultural University, MymensinghGoogle Scholar
  10. Cumagun CJR (2012) Managing plant diseases and promoting sustainability and productivity with Trichoderma. J Agric Sci Technol 14:699–714Google Scholar
  11. Dasgupta MK (1988) Principles of plant pathology. Applied Publisher Pvt Ltd., New DelhiGoogle Scholar
  12. Elham K, Sadravi M, Naeimi S, Khosravi V (2011) Biological control of rice brown spot with native isolates of three Trichoderma. Braz J Microbiol 43:297–305Google Scholar
  13. Fakir GA (2004) An annotated list of seed borne diseases in Bangladesh. Seed Pathology Centre, Department of Plant Pathology, Bangladesh Agricultural University, MymensinghGoogle Scholar
  14. Fakir GA, Mia MAT (2004) The quality of Farmer saved rice seeds in Bangladesh. National workshop on seed health improvement. BARC, DhakaGoogle Scholar
  15. Fattah GMA, Shabana YM, Ismail AE, Rashad YM (2007) Trichoderma harzianum: a biocontrol agent against Bipolaris oryzae. Mycopathologia 164:81–89CrossRefPubMedGoogle Scholar
  16. Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research, 2nd edn. Wiley, New YorkGoogle Scholar
  17. Groth D (2007) The Narrow brown leaf spot outbreak and its control in 2007. Rice Research Station, Crowley, LAGoogle Scholar
  18. Gupta V, Shamas N, Razdan VK, Sharma BC, Sharma R, Kaur K, Chapal S, Johnand DJ, Kumar A (2013) Foliar application of fungicides for the management of brown spot disease in rice (Oryza sativa L.) caused by Bipolaris oryzae. Afr J Agric Res 8:3303–3309Google Scholar
  19. Hasan MM, Chowdhury SP, Alam S, Hossain B, Alam MS (2005) Antifungal effect of plant extracts on seed-borne fungi of wheat seeds regarding seed germination, seedling health and vigour index. Pak J Bio Sci 8:1284–1289CrossRefGoogle Scholar
  20. Hasan MM, Islam MR, Hossain I, Shirin K (2014) Biological control of leaf spot of groundnut. J Biosci Agric Res 1:66–78Google Scholar
  21. Hossain I (2011) BAU-Biofungicide: unique eco-friendly means and new dimension of plant disease control in Bangladesh. Leaflet published from the Dept. of Plant Pathology. Bangladesh Agricultural University, MymensinghGoogle Scholar
  22. Hossain M (2012) Biological control of leaf blight (Bipolaris Sorokiniana) of wheat. Ph.D. Thesis, Bangladesh Agricultural University, MymensinghGoogle Scholar
  23. Hossain I, Dey P, Hossain MZ (2011) Efficacy of Bion, Amistar and Tilt in controlling brown spot and narrow brown spot of rice cv BR11 (Mukta). J Bangladesh Agric Univ 9:201–204Google Scholar
  24. IRRI (1996) Standard evaluation system of rice, 4th ed. International Rice Research Institute, P.O. Box 933,1099 Manila, PhilippinesGoogle Scholar
  25. ISTA (1996) International rules for seed testing. Seed Sci Technol 4:3–49Google Scholar
  26. Jat JG, Agalave HR (2013) Antagonistic properties of Trichoderma species against oilseed-borne fungi. Sci Res Rep 3:171–174Google Scholar
  27. Kalaiselvi S, Panneerselvam A (2015) In-vitro evaluation of fungicides and two species of Trichoderma against Sarocladium oryzae causing sheath rot of paddy (Oryza sativa L). World J Pharmac Res 4:1200–1206Google Scholar
  28. Kamal MM, Mia MAT (2009) Diversity and pathogenicity of the rice brown spot pathogen, bipolaris oryzae (breda de haan) shoem. In Bangladesh assessed by genetic fingerprint analysis. Bangladesh J Bot 38:119–125Google Scholar
  29. Kumar KVK, Raju SK, Reddy MS, Kloepper JW, Lawrence KS, Groth DMME, Sudini H, Du BH (2009) Evaluation of commercially available PGPR for control of rice sheath blight caused Rhizoctonia solani. J Pure Appl Microbiol 3:485–488Google Scholar
  30. Liao QM, Li HY (1994) Study advances of rice false smut in Yunnan province. Yunnan Agric Sci Technol 3:8–12Google Scholar
  31. Liu MX, Oi S, Li Y, Wang S (2009) Study on isolation technique and culture condition of Ustilaginoidea virens. Liaoning Agric Sci 2:20–22Google Scholar
  32. Mahmud H, Hossain I (2013) Eco-friendly management of brown spot (Bipolaris oryzae) of rice and quality seed production. In: 10th International Congress of Plant Pathology. The Chinese Society for Plant Pathology, p. 89Google Scholar
  33. Mahmud H, Hossain I (2016) Effects of plant extracts, BAU-Biofungicide and fungicides on quality and health of seed. Bangladesh J Bot 45:677–684Google Scholar
  34. Manimegalai V, Ambikapathy V, Panneerselvam A (2011) Biological control of paddy brown spot caused by Bipolaris Oryzae (Breda de Haan). Pelagia Research Library. Eur J Exp Biol 1:24–28Google Scholar
  35. Mia MAT, Ahmad MU, Sharma NR, Ali A, Miah SA (1990) Antifungal activity of some plant extracts. Bangladesh J Bot 19:5–10Google Scholar
  36. Naeimi S, Okhovvat SM, Javan-Nikkhah M, Vágvölgyi C, Khosravi V, Kredics L (2010) Biological control of Rhizoctonia solani AG1-1A, the causal agent of rice sheath blight with Trichoderma strains. Phytopathol Mediterr 49:287–300Google Scholar
  37. Nene YL, Thaplial PN (1993) Fungicides in plant disease control. Oxford and IBH publishing Co. PVT. Ltd., New DelhiGoogle Scholar
  38. Nisa TU, Wani AH, Bhat MY, Pala SA, Mir RA (2011) In-vitro inhibitory effect of fungicides and botanicals on mycelia growth and spore germination of Fusarium oxysporum. J Biopest 4:53–56Google Scholar
  39. Ora NAN, Faruq MT, Islam N, Aktar Rahman MM (2011) Detection and identification of seed borne pathogens from some cultivated hybrid rice varieties in Bangladesh. Middle East J Sci Res 10:482–488Google Scholar
  40. Patale SS, Mukadam DS (2011) Management of plant pathogenic fungi by using Trichoderma species. Biosci Dis 2:36–37Google Scholar
  41. Razu MAU, Hossain I (2015) Eco-friendly management of rice diseases. Inter J Appl Sci Biotech 3:80–88CrossRefGoogle Scholar
  42. Sagar SD, Hegde YR, Kulkarni S, Rao MSL (2005) Biocontrol of seed mycoflora of rice. Ann Biol 21:217–220Google Scholar
  43. Sundar AR, Das ND, Krishnaveni D (1995) In-vitro Antagonism of Trichoderma spp. against two fungal pathogens of Castor. Indian J Plant Protect 23:152–155Google Scholar
  44. USDA (2016) World Agricultural Production. United States Department of Agriculture. Foreign Agricultural Services. Circular Series, WAP 12–16Google Scholar

Copyright information

© Botanical Society of Sao Paulo 2017

Authors and Affiliations

  1. 1.Department of Agricultural ExtensionKushtiaBangladesh
  2. 2.Department of Plant PathologyBangladesh Agricultural UniversityMymensinghBangladesh

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