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Brazilian Journal of Botany

, Volume 40, Issue 2, pp 529–537 | Cite as

Genome size variation among and within Ophiopogoneae species by flow cytometric analysis

  • Guangyan Wang
  • Ying Meng
  • Yongping Yang
Original Article
  • 120 Downloads

Abstract

Genome size variation in a taxonomic group reflects evolutionary processes. DNA contents of Ophiopogoneae (40 populations of 31 species) were estimated by flow cytometry. Ploidy levels of Ophiopogon (ten species), Liriope (two species), and Peliosanthes (three species) were determined based on the DNA contents. The genus Peliosanthes showed significant larger genome sizes than Ophiopogon (P < 0.01), and Ophiopogon also significant larger than Liriope (P < 0.05). Intraspecific variation in genome size was mainly chromosome difference. The ITS sequence phylogeny splitted Ophiopogon into two clades, clade I comprising sect. Ophiopogon with diploids and tetraploids, and clade II including transitional species and sects. Ophiopogon and Peliosanthoides with diploids. The trend seemed to increase in genome size from Ophiopogon sect. Peliosanthoides (13.45 pg) to Ophiopogon sect. Ophiopogon (14.27 pg). Polyploidization may be evolutionary direction of Ophiopogon. Our results also suggested that the ‘increase’ hypothesis for genome size evolutionary may hold true in the genus Ophiopogon.

Keywords

DNA content Genome size Ophiopogoneae Phylogeny Ploidy level 

Notes

Acknowledgements

The authors thank Dr. Hu Guangwan for providing certain necessary materials. The study was supported by grants from the Ministry of Science and Technology of China, Major State Basic Research Development Program (2010CB951700), the National Natural Science Foundation of China (NSFC 40930209 to H. Sun), and the General Project of Natural Science Research in Anhui Province (AQKJ2015B018).

Supplementary material

40415_2016_358_MOESM1_ESM.tif (3.9 mb)
Fluorescence histograms illustrating the nuclear DNA content of eight species obtained by flow cytometric analysis of propidium iodide-stained nuclei isolated from silica gel-dried leaves. a. Zea mays; b. O. bodinieri, diploids; c. O. szechuansis, diploids; d. O. latifolius, diploids; e. O. japonicus, tetraploids; f. O. zingiberaceus, tetraploids; g. O. japonicus, tetraploids; h. L. platyphylla, diploids; i. P. ophiopogoniodes, diploids (TIFF 3960 kb)

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Copyright information

© Botanical Society of Sao Paulo 2017

Authors and Affiliations

  • Guangyan Wang
    • 1
    • 2
    • 3
    • 5
  • Ying Meng
    • 2
    • 3
    • 4
  • Yongping Yang
    • 2
    • 3
    • 4
  1. 1.School of Life Sciences, The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest AnhuiAnqing Normal UniversityAnqingChina
  2. 2.Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina
  3. 3.Plant Germplasm and Genomics Center, The Germplasm Bank of Wild Species, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina
  4. 4.Institute of Tibetan Plateau Research at Kunming, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina
  5. 5.University of the Chinese Academy of SciencesBeijingChina

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