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Exogenous hydrogen peroxide up-regulates the contents of ascorbate and glutathione in the leaves of Vigna radiata (Linn.) Wilczek. exposed to salt stress

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Abstract

In this paper, the effect of exogenous hydrogen peroxide (H2O2) on the contents of ascorbate and glutathione in Vigna radiata (Linn.) Wilczek. leaves exposed to salt stress was determined. The findings showed that salt stress increased the activities of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), gamma-glutamylcysteine synthetase (γ-ECS), electrolyte leakage (EL) and the contents of reduced ascorbate (AsA), reduced glutathione (GSH), total ascorbate, total glutathione and malondialdehyde (MDA). Exogenous H2O2 increased the contents of AsA, total ascorbate, GSH and total glutathione through APX, GR, DHAR and γ-ECS in leaves exposed to salt stress. Above increases were also induced by exogenous H2O2 alone, compared with control. Exogenous H2O2 had no effect on the activities of l-galactono-1,4-lactone dehydrogenase (GalLDH) and MDHAR and total ascorbate content. Exogenous H2O2 significantly reduced MDA content and EL induced by salt stress. Above results indicated that exogenous H2O2 could enhance the salt tolerance of V. radiata by regulating the ascorbate and glutathione metabolism.

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Acknowledgements

This study was supported by the Important Scientific Research Project of Henan Institute of Science and Technology (2011010).

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Authors and Affiliations

  1. Henan Institute of Science and Technology, Xinxiang, 453003, China

    Changjuan Shan & Runqiang Liu

  2. Collaborative Innovation Center of Modern Biological Breeding, Xinxiang, 453003, Henan Province, China

    Changjuan Shan

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  1. Changjuan Shan
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  2. Runqiang Liu
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Correspondence to Changjuan Shan.

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Shan, C., Liu, R. Exogenous hydrogen peroxide up-regulates the contents of ascorbate and glutathione in the leaves of Vigna radiata (Linn.) Wilczek. exposed to salt stress. Braz. J. Bot 40, 583–589 (2017). https://doi.org/10.1007/s40415-016-0354-z

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  • DOI: https://doi.org/10.1007/s40415-016-0354-z

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