Brazilian Journal of Botany

, Volume 40, Issue 2, pp 379–388 | Cite as

Comparison of copper and zinc effects on growth, micro- and macronutrients status and essential oil constituents in pennyroyal (Mentha pulegium L.)

  • Hemayat Asgari Lajayer
  • Gholamreza Savaghebi
  • Javad Hadian
  • Mehrnaz Hatami
  • Maryam Pezhmanmehr
Original Article

Abstract

Biosynthesis and metabolism of phytochemicals in aromatic and medicinal plants are vigorously affected by environmental agents. This study was undertaken to investigate the variations in the growth, nutrient status and the essential oils content and composition of pennyroyal (Mentha pulegium L.) plant exposed to different concentrations of copper (Cu; 0, 5, 25 mg kg−1 soil) and zinc (Zn; 0, 10, 50 mg kg−1 soil). The results of the plant treatment responses revealed that the highest plant height, shoot dry weight, essential oil content and yield were achieved in plants treated with Cu and Zn at 5 and 10 mg kg−1. Low Zn concentration showed a synergistic effect on the uptake of Cu, Fe, Mn and K, whereas it revealed **contrary effect on phosphorus (P) status. However, Cu in 5 mg kg−1 poses positive effect on Fe, Mn and P content in root and shoot tissues. Moreover, the addition of Cu and Zn especially at 5 and 10 mg kg−1 was the optimal level in increasing the status of K, Mn, Fe, Cu and Zn in shoot, infusion and decoction. The constituents and quality of M. pulegium essential oils in terms of its major volatile components, namely pulegone, cis-isopulegone, α-pinene, sabinene, 1,8-cineol and thymol, improved significantly with Cu and Zn treatment as compared to control. From this standpoint, M. pulegium can be grown as alternative crop for mildly Cu- and Zn-polluted agricultural soils without contamination of the its industrial products.

Keywords

Cis-isopulegone Essential oil Mentha pulegium Pulegone Trace elements Transfer factor 

References

  1. Adams RP (2001) Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy. Allured Publishing Corporation, Carol StreamGoogle Scholar
  2. Adiloglu S (2007) The effect of increasing nitrogen and zinc doses on the iron, copper and manganese contents of maize plant in calcareous and zinc deficient soils. Agrochimica 51:114–120Google Scholar
  3. Aghel N, Yamini YA, Hadjiakhoondi S, Pourmortazavi M (2004) Supercritical carbon dioxide extraction of Mentha pulegium L. essential oil. Talanta 62:407–411CrossRefPubMedGoogle Scholar
  4. Alaoui-Sosse B, Genet P, Vinit-Dunand F, Toussaint ML, Epron D, Badot PM (2004) Effect of copper on growth in cucumber plants (Cucumis sativus) and its relationships with carbohydrate accumulation and changes in ion contents. Plant Sci 166:1213–1218CrossRefGoogle Scholar
  5. Alloway BJ (2004) Zinc in soils and crop nutrition. International Zinc Association Brussels, BrusselsGoogle Scholar
  6. Angelova V, Ivanov K, Ivanova R (2006) Heavy metal content in plants from family Lamiaceae cultivated in an industrially polluted region. J Herbs Spices Med Plants 11:37–46CrossRefGoogle Scholar
  7. Balamurugan S, Thiyagarajan G, Manivasagaperumal R, Vijayarengan P (2011) Effect of copper on growth, dry matter yield and nutrient content of Vigna radiata L. Wilczek. J Phytol 3:53–62Google Scholar
  8. Bernal M, Cases R, Picorel R, Yruela I (2007) Foliar and root Cu supply affect differently Fe-and Zn-uptake and photosynthetic activity in soybean plants. Environ Exp Bot 60:145–150CrossRefGoogle Scholar
  9. Chaney R (1993) Zinc phytotoxicity. Zinc in soils and plants. Springer, Berlin, pp 135–150CrossRefGoogle Scholar
  10. Chapman HD, Pratt PF (1978) Methods of analysis for soils, plants, and water. Univ Calif Publ 4034:70–72Google Scholar
  11. Commission CA (2001) Food additives and contaminants. Joint FAO, WHO food standards programme, ALINORM, 1, Rome, ItalyGoogle Scholar
  12. Cottenie A (1980) Soil and plant testing as a basis of fertilizer recommendations (FAO Soils Bulletin38/2). Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
  13. Demirevska-Kepova K, Simova-Stoilova L, Stoyanova Z, Holzer R, Feller U (2004) Biochemical changes in barley plants after excessive supply of copper and manganese. Environ Exp Bot 52:253–266CrossRefGoogle Scholar
  14. El-Sawi SA, Mohamed M (2002) Cumin herb as a new source of essential oils and its response to foliar spray with some micro-elements. Food Chem 77:75–80CrossRefGoogle Scholar
  15. Elzaawely AA, Xuan TD, Tawata S (2007) Changes in essential oil, kava pyrones and total phenolics of Alpinia zerumbet (Pers.) BL Burtt. & RM Sm. leaves exposed to copper sulphate. Environ Exp Bot 59:347–353CrossRefGoogle Scholar
  16. Figueiredo AC, Barroso JG, Pedro LG, Scheffer JJ (2008) Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour Fragr J 23:213–226CrossRefGoogle Scholar
  17. Hacisalihoglu G, Kochian LV (2003) How do some plants tolerate low levels of soil zinc? Mechanisms of zinc efficiency in crop plants. New Phytol 159:341–350CrossRefGoogle Scholar
  18. Hendawy S, Khalid A (2005) Response of sage (Salvia officinalis L.) plants to zinc application under different salinity levels. J Appl Sci Res 1:147–155Google Scholar
  19. Kabata-Pendias A (2000) Trace elements in soils and plants, 3rd edn. CRC Press, Boca Raton, p 432CrossRefGoogle Scholar
  20. Kamkar A, Javan AJ, Asadi F, Kamalinejad M (2010) The antioxidative effect of Iranian Mentha pulegium extracts and essential oil in sunflower oil. Food Chem Toxicol 48:1796–1800CrossRefPubMedGoogle Scholar
  21. Kovacik J, Klejdus B, Hedbavny J, Stork F, Backor M (2009) Comparison of cadmium and copper effect on phenolic metabolism, mineral nutrients and stress-related parameters in Matricaria chamomilla plants. Plant Soil 320:231–242CrossRefGoogle Scholar
  22. Marschner H (2002) Mineral nutrition of higher plants. Elsevier, AmsterdamGoogle Scholar
  23. Misra A, Srivastava A, Srivastava N, Khan A (2005) Zn-acquisition and its role in growth, photosynthesis, photosynthetic pigments, and biochemical changes in essential monoterpene oil (s) of Pelargonium graveolens. Photosynthetica 43:153–155CrossRefGoogle Scholar
  24. Pandey S, Gupta K, Mukherjee AK (2007) Impact of cadmium and lead on Catharanthus roseus- A phytoremediation study. J Environ Biol 28:655–662PubMedGoogle Scholar
  25. Peralta-Videa J, Gardea-Torresdey J, Gomez E, Tiemann K, Parsons J, Carrillo G (2002) Effect of mixed cadmium, copper, nickel and zinc at different pHs upon alfalfa growth and heavy metal uptake. Environ Pollut 119:291–301CrossRefPubMedGoogle Scholar
  26. Pytlakowska K, Kita A, Janoska P, Połowniak M, Kozik V (2012) Multi-element analysis of mineral and trace elements in medicinal herbs and their infusions. Food Chem 135:494–501CrossRefPubMedGoogle Scholar
  27. Rodrigues L, Povoa O, Teixeira G, Figueiredo AC, Moldao M, Monteiro A (2013) Trichomes micromorphology and essential oil variation at different developmental stages of cultivated and wild growing Mentha pulegium L. populations from Portugal. Ind Crops Prod 43:692–700CrossRefGoogle Scholar
  28. Rubio C, Lucas J, Gutierrez A, Glez-Weller D, Marrero BP, Caballero J, Revert C, Hardisson A (2012) Evaluation of metal concentrations in mentha herbal teas (Menta piperita, Mentha pulegium and Mentha species) by inductively coupled plasma spectrometry. J Pharm Biomed Anal 71:11–17CrossRefPubMedGoogle Scholar
  29. Scora R, Chang A (1997) Essential oil quality and heavy metal concentrations of peppermint grown on a municipal sludge-amended soil. J Environ Qual 26:975–979CrossRefGoogle Scholar
  30. Sharma RK, Agrawal M, Marshall FM (2009) Heavy metals in vegetables collected from production and market sites of a tropical urban area of India. Food Chem Toxicol 47:583–591CrossRefPubMedGoogle Scholar
  31. Street R (2012) Heavy metals in medicinal plant products-An African perspective. S Afr J Bot 82:67–74CrossRefGoogle Scholar
  32. Teixeira B, Marquesa A, Ramosa C, Batista I, Serrano C, Matos O, Neng N, Nogueira JMF, Saraiva JA, Nunes ML (2012) European pennyroyal (Mentha pulegium) from Portugal: chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil. Ind Crops Prod 36:81–87CrossRefGoogle Scholar
  33. Zheljazkov VD, Nielsen NE (1996) Studies on the effect of heavy metals (Cd, Pb, Cu, Mn, Zn and Fe) upon the growth, productivity and quality of lavender (Lavandula angustifolia Mill.) production. J Essent Oil Res 8:259–274CrossRefGoogle Scholar
  34. Zheljazkov VD, Craker LE, Xing B (2006) Effects of Cd, Pb, and Cu on growth and essential oil contents in dill, peppermint, and basil. Environ Exp Bot 58:9–16CrossRefGoogle Scholar
  35. Zheljazkov VD, Craker LE, Xing B, Nielsen NE, Wilcox A (2008) Aromatic plant production on metal contaminated soils. Sci Total Environ 395:51–62CrossRefPubMedGoogle Scholar
  36. Zheljazkov VD, Cerven V, Cantrell CL, Ebelhar WM, Horgan T (2009) Effect of nitrogen, location, and harvesting stage on peppermint productivity, oil content, and oil composition. HortScience 44:1267–1270Google Scholar
  37. Zheljazkov VD, Cantrell CL, Astatkie T, Cannon JB (2011) Lemongrass productivity, oil content, and composition as a function of nitrogen, sulfur, and harvest time. Agron J 103:805–812CrossRefGoogle Scholar

Copyright information

© Botanical Society of Sao Paulo 2016

Authors and Affiliations

  • Hemayat Asgari Lajayer
    • 1
  • Gholamreza Savaghebi
    • 2
  • Javad Hadian
    • 3
  • Mehrnaz Hatami
    • 4
  • Maryam Pezhmanmehr
    • 5
  1. 1.Department of Soil Science, Faculty of AgricultureUniversity of TabrizTabrizIran
  2. 2.Department of Soil Science Engineering, University College of Agriculture and Natural ResourcesUniversity of TehranKarajIran
  3. 3.Medicinal Plants and Drugs Research InstituteShahid Beheshti University, G.C.EvinIran
  4. 4.Department of Medicinal Plants, Faculty of Agriculture and Natural ResourcesArak UniversityArakIran
  5. 5.Department of Horticulture, Science and Research BranchIslamic Azad UniversityTehranIran

Personalised recommendations