Abstract
Triacontanol (TRIA) has been shown to regulate growth in some crop species exposed to stressful environments. In the present study, we assessed whether or not and to what extent foliar-applied TRIA can alter metabolism and nutrient uptake, and thus induce salt tolerance in maize (Zea mays L.). For this purpose, 14-day-old plants of two maize cultivars (MMRI-Yellow and Hybrid S-515) were subjected to salt stress (0 mM and 100 mM NaCl). 21-day-old plants were exogenously treated with different concentrations (0, 2, 5 µM) of TRIA. After 51 days of TRIA treatment (72-day-old plants), data of various attributes were collected. Generally, salt stress negatively affected the growth attributes in both maize cultivars. Furthermore, salinity increased relative membrane permeability (RMP), malondialdehyde (MDA), total soluble protein contents, free proline and sodium (Na+) contents, and the activities of peroxidase (POD) and catalase (CAT) in both maize cultivars. Foliar spray of TRIA enhanced growth, soluble proteins, nitrate reductase (NR) activity, total phenolics, and free proline and shoot K+ contents, while it decreased RMP and root Na+ contents in maize. Overall, cv. MMRI-Yellow showed greater tolerance to salinity compared with Hybrid S-515. Among the various TRIA levels, 5 µM was much more effective in increasing growth of maize plants under both normal and saline regimes.
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Aftab T, Khan MMA, Idrees M, Naeem M, Singh M, Ram M (2010) Stimulation of crop productivity, photosynthesis and artemisinin production in Artemisia annua L. by triacontanol and gibberellic acid application. J Plant Interact 4:273–281
Allen SK, Dobrenz AK, Schonhorst MH, Stoner JE (1985) Heritability of NaCl tolerance in germinating alfalfa seeds. Agron J 77:90–96
Arnon DT (1949) Copper enzyme in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15
Aziz R, Shahbaz M, Ashraf M (2013) Influence of foliar application of triacontanol on growth attributes, gas exchange and chlorophyll fluorescence in sunflower (Helianthus annuus L.) under saline stress. Pak J Bot 45:1913–1918
Bates IS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207
Beevers L, Hageman RH (1969) Nitrate reduction and its role in nitrate assimilation in plants. Physiol Plant 74:214–219
Ben Rejeb KB, Abdelly C, Savoure A (2014) How reactive oxygen species and proline face stress together. Plant Physiol Biochem 80:278–284
Borowski E, Blamowski ZK (2009) The effect of triacontanol ‘TRIA’ and Asahi-SL on the development and metabolic activity of sweet basil (Ocimum basilicum L.) plants treated with chilling. Folia Hort 21:39–48
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Ana Biochem 72:248–254
Carleton AE, Foote WH (1965) A comparison of methods for estimating total leaf area of barley plants. Crop Sci 5:602–603
Carmak I, Horst JH (1991) Effects of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol Plant 83:463–468
Chance B, Maehly A (1955) Assay of catalase and peroxidase. Meth Enzymol 2:764–817
Charest C, Phan CT (1990) Cold acclimation of wheat (Triticum aestivum) properties of enzymes involved in proline metabolism. Physiol Plant 80:159–168
Charlton JL, Hunter NR, Green NA, Fritzi W, Addisoni BM, Woodburyz W (1980) The effects of triacontanol and triacontanol derivatives on germination and seedling growth of Leeds durum wheat. Can J Plant Sci 60:795–797
Chen X, Yuan H, Chen R, Zhu L, He G (2003) Biochemical and photochemical changes in response to triacontanol in rice (Oryza sativa L.). Plant Growth Regul 40:249–256
Dheera S, Ekta K, Malik CP (2012) Effect of drought stress and its interaction with two phytohormones on Vigna radiata seed germination and seedling growth. Int J Life Sci 1:201–207
Garg SK (2013) Role and hormonal regulation of nitrate reductase activity in higher plants: a review. Plant Sci Feed 3:13–20
Giannopolitis CN, Ries SK (1977) Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol 59:309–314
Giberti S, Funck D, Forlani G (2014) Δ1-pyrroline-5-carboxylate reductase from Arabidopsis thaliana: stimulation or inhibition by chloride ions and feedback regulation by proline depend on whether NADPH or NADH acts as co-substrate. New Phytol 202:911–919
Grieve CM, Grattan SR (1983) Rapid assay for the determination of water soluble quaternary ammonium compounds. Plant Soil 70:303–307
Grzegorczyk I, Bilichowski I, Mikicuik-Olasik E, Wysokinska H (2005) In vitro cultures of Salvia officinalis L. as a source of antioxidant compounds. Acta Soc Bot Pol 74:17–21
Grzegorczyk I, Bilichowski I, Mikicuik-Olasik E, Wysokinska H (2006) The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L. Acta Soc Bot Pol 75:11–15
Hoagland RE (1980) Effects of triacontanol on seed germination and early growth. Bot Gaz 141:53–55
Iqbal M, Ashraf M (2013) Gibberellic acid mediated induction of salt tolerance in wheat plants: growth, ionic partitioning, photosynthesis, yield and hormonal homeostasis. Environ Exp Bot 86:76–85
Jaworski EG (1971) Nitrate reductase assay in intact plant tissue. Biochem Biophys Res Commun 43:1274–1279
Julkenen-Titto R (1985) Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. J Agric Food Chem 33:213–217
Kaya C, Sonmez O, Aydemir S, Ashraf M, Dikilitas M (2013) Exogenous application of mannitol and thiourea regulates plant growth and oxidative stress responses in salt-stressed maize (Zea mays L.). J Plant Interact 8:234–241
Khan MMA, Bhardwaj G, Naeem M, Moinuddin Mohammad F, Singh M, Nasir S, Idrees M (2009) Response of tomato (Solanum lycopersicum L.) to application of potassium and triacontanol. Acta Hortic (ISHS) 823:199–208
Khan NA, Khan MIR, Asgher M, Fatma M, Masood A, Syeed S (2014) Salinity tolerance in plants: revisiting the role of sulfur metabolites. J Plant Biochem Physiol 2:120. doi:10.4172/2329-9029.1000120
Krishnan RR, Kumari BDR (2008) Effect of n-triacontanol on the growth of salt stressed soyabean plants. J Biosci 19:53–56
Moore S, Stein WH (1957) A modified ninhydrin Reagent for the photometric determination of amino acids and related compounds. J Biol Chem 211:907–913
MSTAT Development Team (1989) MSTAT user’s guide: A microcomputer program for the design management and analysis of agronomic research experiments. Michigan State University, East Lansing
Muthuchelian K, Murugan C, Harigovindan R, Nedunchezhian N, Kulandaivelu G (1996) Ameliorating effect of triacontanol on salt stressed Erythrina variegata seedlings. Changes in growth, biomass, pigments and solute accumulation. Biol Plant 38:133–136
Naeem M, Khan MMA, Moinuddin Idrees M, Aftab T (2010) Changes in photosynthesis, enzyme activities and production of anthraquinone and sennoside content of coffee senna (Senna occidentalis L.) by triacontanol. Int J Plant Dev Biol 4:53–59
Naeem M, Khan MMA, Moinuddin Idrees M, Aftab T (2011) Triacontanol-mediated regulation of growth and other physiological attributes, active constituents and yield of Mentha arvensis L. Plant Growth Regul 65:195–206
Ozden M, Demirel U, Kahraman A (2009) Effects of proline on antioxidant system in leaves of grapevine (Vitis vinifera L.) exposed to oxidative stress by H2O2. Sci Hort-Amsterdam 119:163–168
Perveen S, Shahbaz M, Ashraf M (2010) Regulation in gas exchange and quantum yield of photosystem II (PSII) in salt-stressed and non-stressed wheat plants raised from seed treated with triacontanol. Pak J Bot 42:3073–3081
Perveen S, Shahbaz M, Ashraf M (2011) Modulation in activities of antioxidant enzymes in salt stressed and non-stressed wheat (Triticum aestivum L.) plants raised from seed treated with triacontanol. Pak J Bot 43:2463–2468
Perveen S, Shahbaz M, Ashraf M (2012a) Is pre-sowing seed treatment with triacontanol effective in improving some physiological and biochemical attributes of wheat (Triticum aestivum L.) under salt stress? J Appl Bot Food Qual 85:41–48
Perveen S, Shahbaz M, Ashraf M (2012b) Changes in mineral composition, uptake and use efficiency of salt stressed wheat (Triticum aestivum L.) plants raised from seed treated with triacontanol. Pak J Bot 44:27–35
Perveen S, Shahbaz M, Ashraf M (2013) Influence of foliar-applied triacontanol on growth, gas exchange characteristics, and chlorophyll fluorescence at different growth stages in wheat under saline conditions. Photosynthetica 51:541–551
Perveen S, Shahbaz M, Ashraf M (2014) Triacontanol-induced changes in growth, yield, leaf water relations, antioxidative defense system and some key osmoprotectants in bread wheat (Triticum aestivum L.) under saline stress. Turk J Bot 38:896–913
Posmyk MM, Kontek R, Janas KM (2009) Antioxidant enzymes activity and phenolic compounds content in red cabbage seedlings exposed to copper stress. Ecotox Environ Safe 72:596–602
Ramanarayan K, Bhut A, Shripathi V, Swamy GS, Rao KS (2000) Triacontanol inhibits both enzymatic and nonenzymatic lipid peroxidation. Phytochem 55:59–66
Ries SK, Richman TL, Wert VF (1978) Growth and yield of crops treated with triacontanol. J Am Soc Hort Sci 103:361–364
Ries S, Savithiry S, Wert V, Widders I (1993) Rapid induction of ion pulses in tomato, cucumber, and maize plants following a foliar application of L(+)-Adenosine. Plant Physiol 101:49–55
Shahbaz M, Ashraf M (2013) Improving salinity tolerance in cereals. Cri Rev Plant Sci 32:237–249
Shekoofeh E, Shahla S (2012) Influence of salicylic acid on growth and some biochemical parameters in a C4 plant (Panicum miliaceum L.) under saline conditions. Afr J Biotechnol 11:621–627
Singh M, Khan MMA, Moinuddin, Naeem M (2011) Augmentation of nutraceuticals, productivity and quality of ginger (Zingiber officinale Rosc.) through triacontanol application. Plant Biosyst 146:106–113
Snedecor GW, Cochran GW (1980) Statistical Methods, 7th edn. The Lowa State University Press, Lowa
Soda N, Wallace S, Karan R (2015) Omics study for abiotic stress responses in plants. Adv Plants Agric Res 2(1):00037. doi:10.15406/apar.2015.02.00037
Szabados L, Savoure A (2010) Proline: a multifunctional amino acid. Trends Plant Sci 15:89–97
Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective roles of exogenous polyamines. Plant Sci 151:59–66
Verma A, Malik CP, Gupta VK, Bajaj BK (2011) Effects of in vitro triacontanol on growth, antioxidant enzymes, and photosynthetic characteristics in Arachis hypogaea L. Braz J Plant Physiol 23:271–277
Yang G, Rhodes G, Joly RG (1996) Effects of high temperature on membrane stability and chlorophyll fluorescence in glycinebetaine-deficient and glycinebetaine-containing maize lines. Aust J Plant Physiol 23:437–443
Zadebagheri M, Azarpanah A, Javanmardi S (2014) Proline metabolite transport an efficient approach in corn yield improvement as response to drought conditions. Int J Farm Alli Sci 3:453–461
Zulfiqar S, Shahbaz M (2013) Modulation in gas exchange parameters and photosystem-II activity of canola (Brassica napus L.) by foliar-applied triacontanol under salt stress. Agrochimica 57:193–200
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The current study is part of the project No. PD-IPFP/HRD/HEC/2013/1117, financially supported by Higher Education Commission (HEC) of Pakistan.
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Perveen, S., Iqbal, M., Parveen, A. et al. Exogenous triacontanol-mediated increase in phenolics, proline, activity of nitrate reductase, and shoot k+ confers salt tolerance in maize (Zea mays L.). Braz. J. Bot 40, 1–11 (2017). https://doi.org/10.1007/s40415-016-0310-y
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DOI: https://doi.org/10.1007/s40415-016-0310-y