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Polyamine, amino acid, and carbohydrate profiles during seed storage of threatened woody species of the Brazilian Atlantic Forest may be associated with seed viability maintenance

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Abstract

Due to the economic importance of wood production, species including Cedrela fissilis Vell. and Cariniana legalis (Mart.) Kuntze have been heavily exploited and are currently threatened with extinction. In addition, these species produce seeds with low viability when stored for long periods. In this work, the effect of storage time on seedling emergence and alterations in the levels of biomolecules such as carbohydrates, polyamines (PAs), and amino acids were analyzed in C. fissilis and C. legalis seeds. Seedling emergence and the emergence speed index were significantly reduced during seed storage in both species, with a greater reduction for C. legalis seeds. Sucrose was detected in both species, with the highest levels in C. fissilis seeds, whereas the monosaccharides glucose and fructose were detected only in C. legalis seeds. Higher levels of putrescine (Put) were observed in C. legalis seeds than in C. fissilis seeds. In contrast, C. fissilis seeds showed a significant increase in most of the amino acids analyzed. These results suggest that the presence of glucose and fructose, the higher level of Put, and the lower levels of amino acids observed in C. legalis seeds may be associated with greater reductions in vigor and seedling emergence compared with C. fissilis seeds. This is the first report showing these relationships among carbohydrates, PAs, and amino acids in these species and may be useful for further studies on seed conservation of timber species.

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Acknowledgments

This research was supported by the Carlos Chagas Filho Foundation for Research Support in the State of Rio de Janeiro—FAPERJ (E26/111.389-2012, E26/102.989/2012, and E26/010.001507/2014), and the National Council for Scientific and Technological Development—CNPq (305645/2013-7 and 444453/2014-8). Scholarships were granted by the Coordination for the Improvement of Higher Education Personnel—CAPES to KRS, RSR, and VPMA.

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Authors and Affiliations

  1. Laboratório de Biologia Celular e Tecidual (LBCT), Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense “Darcy Ribeiro” (UENF), Campos Dos Goytacazes, RJ, 28013-602, Brazil

    Kariane Rodrigues Sousa, Victor Paulo Mesquita Aragão & Claudete Santa-Catarina

  2. Laboratório de Biotecnologia (LBT), Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense “Darcy Ribeiro” (UENF), Campos Dos Goytacazes, RJ, 28013-602, Brazil

    Ricardo Souza Reis & Vanildo Silveira

  3. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, Universidade Estadual do Norte Fluminense “Darcy Ribeiro” (UENF), Campos Dos Goytacazes, RJ, 28013-602, Brazil

    Ricardo Souza Reis & Vanildo Silveira

  4. Laboratório de Biologia Celular de Planta (BIOCEL), Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, 05508-090, Brazil

    Amanda Ferreira Macedo & Eny Iochevet Segal Floh

  5. Laboratório de Fitotecnia (LFIT), Setor de Tecnologia de Sementes, Universidade Estadual do Norte Fluminense “Darcy Ribeiro” (UENF), Campos Dos Goytacazes, RJ, 28013-602, Brazil

    Henrique Duarte Vieira

  6. Laboratório de Engenharia Agrícola (LEAG), Centro de Ciências e Tecnologias Agropecuárias (CCTA), Universidade Estadual do Norte Fluminense “Darcy Ribeiro” (UENF), Campos Dos Goytacazes, RJ, 28013-602, Brazil

    Cláudio Luiz Melo de Souza

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  1. Kariane Rodrigues Sousa
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  2. Victor Paulo Mesquita Aragão
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  3. Ricardo Souza Reis
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  4. Amanda Ferreira Macedo
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Correspondence to Claudete Santa-Catarina.

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Sousa, K.R., Aragão, V.P.M., Reis, R.S. et al. Polyamine, amino acid, and carbohydrate profiles during seed storage of threatened woody species of the Brazilian Atlantic Forest may be associated with seed viability maintenance. Braz. J. Bot 39, 985–995 (2016). https://doi.org/10.1007/s40415-016-0293-8

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