Abstract
Physalis angulata L. is an annual herb of Solanaceae. Among several biological activities described for this species, its cytotoxic action is due to the presence of flavonoids. Natural infection of P. angulata by Potato virus Y, strain O (PVYO), has already been described. The main goal of this study is to verify whether PVYO infection influences the secondary metabolism of P. angulata, evaluating total amount of phenolic compounds and the content and profiles of flavonoids. Twenty-day-old plants were distributed into three groups: control (C1), mechanically injured (C2), and artificially inoculated (PVYO). After 21 days, leaves from local and systemic infection were collected and compounds were analyzed by UV–Vis and HPLC. Five flavonoids were partially identified as rutin, kaempferol-4′-O-ramnoglucosyl, kaempferol-3-O-ramnoglucosyl, and two other kaempferol derivatives. Quantitative analysis of total phenolic compounds and total flavonoid in the local infection were similar, with higher percentage in the C1 group, followed by the PVYO group and, consequently, the lowest percentage present in C2 group. There were no qualitative differences in flavonoid profiles between healthy and infected groups. However, lower concentrations of these compounds were detected in the systemic infection of infected plants, suggesting that PVYO influences secondary metabolism by reducing the amounts of flavonoids.
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Acknowledgments
We are grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for fellowship to Alice Nagai (Process: 134587/2010-3) and to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support. DYACS was supported by a CNPq research fellowship.
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Nagai, A., Duarte, L.M.L., Chaves, A.L.R. et al. Potato virus Y infection affects flavonoid profiles of Physalis angulata L.. Braz. J. Bot 38, 729–735 (2015). https://doi.org/10.1007/s40415-015-0181-7
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DOI: https://doi.org/10.1007/s40415-015-0181-7