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Identification of quantitative trait loci for Na+, K+ and Ca++ accumulation traits in rice grown under saline conditions using F2 mapping population

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Abstract

Salinity affects rice production worldwide. Quantitative trait loci (QTL) for different sodium (Na+), potassium (K+), and calcium (Ca++) accumulation traits were detected using 113 F2 individuals derived from a cross between IR36 (salt-susceptible) and Pokkali (salt-tolerant) rice cultivars. 17 QTLs were detected; three under control conditions, three under saline and 11 under relative value datasets. These QTLs were located on chromosomes 1, 3, 4, 5, 6, 7, 8 and 11, respectively. The R 2 (phenotypic variance explained) value for these QTLs ranged from 7.69 to 72.57 %. The QTLs identified on chromosomes 7 and 8 were novel as there were no previous reports of QTLs for Na+ uptake (SU) and K+ uptake (PU) for these genomic regions. PU and K+ uptake ratio (PUR) had significant positive correlation with grain yield under saline conditions. The QTLs identified for PU and PUR (QTLpur-3, QTLpurr-5-1, QTLpurr-5-2) will be helpful for increasing rice grain yield under salt stress conditions. A major locus controlling Na+ uptake (SU) (QTLsur-7) was identified on chromosome 7, with R 2 value of 72.57 %. This may be a major gene involved in SU. Additive effect of the salt-tolerant rice cultivar, Pokkali alleles increased this trait by 0.16 %. This QTL is important with respect to Na+ exclusion and may be a good candidate for marker-assisted selection.

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Acknowledgments

The first author was a PhD scholar on the prime minister’s scholarship programme financed by Government of Pakistan (Ministry of Education). This work is the output of his PhD research project. We are thankful to Punjab Agricultural Research Board (PARB), Lahore, Pakistan for providing financial support to construct salinity blocks and purchase instruments and chemicals.

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Authors and Affiliations

  1. School of Biological Sciences, University of the Punjab, Lahore, Pakistan

    Md. Shah Kamal Khan & Javed Iqbal

  2. Department of Botany, Faculty of Science and Technology, Government College University, Faisalabad, Pakistan

    Muhammad Saeed

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  1. Md. Shah Kamal Khan
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  2. Muhammad Saeed
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  3. Javed Iqbal
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Correspondence to Muhammad Saeed.

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Khan, M.S.K., Saeed, M. & Iqbal, J. Identification of quantitative trait loci for Na+, K+ and Ca++ accumulation traits in rice grown under saline conditions using F2 mapping population. Braz. J. Bot 38, 555–565 (2015). https://doi.org/10.1007/s40415-015-0160-z

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  • DOI: https://doi.org/10.1007/s40415-015-0160-z

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