Abstract
Purpose
The present study was undertaken to evaluate cytomegalovirus (CMV) load in carious lesions in children with severe early childhood caries (S-ECC) and compare it with caries-free (CF), healthy children, by quantitative real-time polymerase chain reaction (PCR).
Materials and methods
Dental plaque samples were collected from 57 subjects including S-ECC (n = 33) and CF (n = 24) patients. The two groups were matched in terms of age, gender, and socioeconomic status. After deoxyribonucleic acid (DNA) extraction, SYBR Green real-time PCR was used to quantify CMV DNA.
Results
The CMV DNA was detected in seven patients in the S-ECC group. The mean of decayed, missing, and filled tooth surfaces (dmfs) was 11.90 ± 5.95 in the S-ECC group. There was no significant difference in gender between the two groups. There was a significant relationship between CMV positivity in children and the S-ECC group (p = 0.043).
Conclusion
Results of this study suggest a relationship between the presence of CMV in the oral cavity and S-ECC. We can design clinical trials to confirm these results and improve children's dental care.
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Availability of data and materials
Dataset and analysis is available through the corresponding author.
Code availability
SPSS software V 20.
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This work was funded by Isfahan University of Medical Sciences.
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Nourbakhsh, N., Sadri, L., Moghim, S. et al. Comparative real-time PCR quantification of cytomegalovirus in severe early childhood caries and caries-free children. Eur Arch Paediatr Dent 23, 797–801 (2022). https://doi.org/10.1007/s40368-022-00711-z
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DOI: https://doi.org/10.1007/s40368-022-00711-z